Sprouty genes are essential for the normal development of epibranchial ganglia in the mouse embryo.
Bottom Line: Fibroblast growth factor (FGF) signalling has important roles in the development of the embryonic pharyngeal (branchial) arches, but its effects on innervation of the arches and associated structures have not been studied extensively.However, epithelial-specific gene deletion only results in defects in the facial nerve and not the glossopharyngeal and vagus nerves, suggesting that the facial nerve is most sensitive to perturbations in RTK signalling.Reducing the Fgf8 gene dosage only partially rescued defects in the glossopharyngeal nerve and was not sufficient to rescue facial nerve defects, suggesting that FGF8 is functionally redundant with other RTK ligands during facial nerve development.
Affiliation: Department of Craniofacial Development, King's College London, Floor 27, Guy's Tower, London, SE1 9RT, UK.Show MeSH
Related in: MedlinePlus
Mentions: 2D lateral pharyngeal arch area was measured using pictures taken on a Nikon SMZ1500 microscope at a resolution of 2560 × 1920 pixels. Measurements were normalised to the total embryo length to account for variations in embryonic size as indicated in Suppl. Fig. 2. Adobe Photoshop (Adobe Systems Incorporated) was used to measure 2D pharyngeal arch area in pixels. Statistical analysis included the D'Agostino and Pearson omnibus normality test, the unpaired two-tailed t-test and the two-tailed Mann–Whitney test (Prism 5, Graphpad software Inc).
Affiliation: Department of Craniofacial Development, King's College London, Floor 27, Guy's Tower, London, SE1 9RT, UK.