Sprouty genes are essential for the normal development of epibranchial ganglia in the mouse embryo.
Bottom Line: Fibroblast growth factor (FGF) signalling has important roles in the development of the embryonic pharyngeal (branchial) arches, but its effects on innervation of the arches and associated structures have not been studied extensively.However, epithelial-specific gene deletion only results in defects in the facial nerve and not the glossopharyngeal and vagus nerves, suggesting that the facial nerve is most sensitive to perturbations in RTK signalling.Reducing the Fgf8 gene dosage only partially rescued defects in the glossopharyngeal nerve and was not sufficient to rescue facial nerve defects, suggesting that FGF8 is functionally redundant with other RTK ligands during facial nerve development.
Affiliation: Department of Craniofacial Development, King's College London, Floor 27, Guy's Tower, London, SE1 9RT, UK.Show MeSH
Related in: MedlinePlus
Mentions: Whole E10.5 embryos were stained with an antibody to neurofilament to reveal the developing cranial nerves (Figs. 2A–E′). Cranial nerves in Spry1−/− (n = 9/10) and Spry2−/− (n = 8/8) embryos exhibited normal morphologies when compared to wildtype controls (n = 10) at E10.5 (compare Figs. 2B and C with A, Table 1). Most Spry+/−;Spry2+/− embryos (n = 28/34) also exhibited no cranial nerve defects, confirming that the loss of two Sprouty alleles is insufficient to cause significant cranial nerve defects (Fig. 2D). These observations suggest that Spry1 and Spry2 may functionally compensate for the loss of each other during branchial nerve development.
Affiliation: Department of Craniofacial Development, King's College London, Floor 27, Guy's Tower, London, SE1 9RT, UK.