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Sprouty genes are essential for the normal development of epibranchial ganglia in the mouse embryo.

Simrick S, Lickert H, Basson MA - Dev. Biol. (2011)

Bottom Line: Fibroblast growth factor (FGF) signalling has important roles in the development of the embryonic pharyngeal (branchial) arches, but its effects on innervation of the arches and associated structures have not been studied extensively.However, epithelial-specific gene deletion only results in defects in the facial nerve and not the glossopharyngeal and vagus nerves, suggesting that the facial nerve is most sensitive to perturbations in RTK signalling.Reducing the Fgf8 gene dosage only partially rescued defects in the glossopharyngeal nerve and was not sufficient to rescue facial nerve defects, suggesting that FGF8 is functionally redundant with other RTK ligands during facial nerve development.

View Article: PubMed Central - PubMed

Affiliation: Department of Craniofacial Development, King's College London, Floor 27, Guy's Tower, London, SE1 9RT, UK.

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Related in: MedlinePlus

Dynamic Spry1 and Spry2 expression in the developing pharyngeal region. Sprouty gene expression analysed by RNA in situ hybridisation. Spry1 and Spry2 expression in whole mount E8.5 (8–9ss) (A,B), E9 (16–20ss) (C,D) and E9.5 (25–26ss) (F,G) embryos, anterior to the top and ventral to the right. Embryos in A and B were sectioned as indicated by the red line and sections are shown in A′ and B′, respectively. Note Spry1 and Spry2 expression in the ectoderm (ecto), mesoderm (meso) and endoderm (endo) at E8.5. Ngn2 expression is shown to indicate the position of the epibranchial placodes at E9 (E) and E9.5 (H) for comparison. The first (PA1), second (PA2) and third (PA3) pharyngeal arches; otic vesicle (OV); geniculate (G), petrosal (P) and nodose (N) placodes are labelled.
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f0005: Dynamic Spry1 and Spry2 expression in the developing pharyngeal region. Sprouty gene expression analysed by RNA in situ hybridisation. Spry1 and Spry2 expression in whole mount E8.5 (8–9ss) (A,B), E9 (16–20ss) (C,D) and E9.5 (25–26ss) (F,G) embryos, anterior to the top and ventral to the right. Embryos in A and B were sectioned as indicated by the red line and sections are shown in A′ and B′, respectively. Note Spry1 and Spry2 expression in the ectoderm (ecto), mesoderm (meso) and endoderm (endo) at E8.5. Ngn2 expression is shown to indicate the position of the epibranchial placodes at E9 (E) and E9.5 (H) for comparison. The first (PA1), second (PA2) and third (PA3) pharyngeal arches; otic vesicle (OV); geniculate (G), petrosal (P) and nodose (N) placodes are labelled.

Mentions: To determine whether Sprouty genes may have a function in the development of the epibranchial ganglia in the mouse, we analysed the expression of Spry1 and Spry2 between embryonic day (E)8.5 and E9.5 of development. At E8.5, Spry1 and Spry2 are both expressed in the pharyngeal ectoderm, endoderm and mesoderm (Figs. 1A–B′). This pattern of expression is consistent with the expression of several Fgf genes in these tissues at this time of development and the role of FGF signalling in the induction of otic fate (Ladher et al., 2010).


Sprouty genes are essential for the normal development of epibranchial ganglia in the mouse embryo.

Simrick S, Lickert H, Basson MA - Dev. Biol. (2011)

Dynamic Spry1 and Spry2 expression in the developing pharyngeal region. Sprouty gene expression analysed by RNA in situ hybridisation. Spry1 and Spry2 expression in whole mount E8.5 (8–9ss) (A,B), E9 (16–20ss) (C,D) and E9.5 (25–26ss) (F,G) embryos, anterior to the top and ventral to the right. Embryos in A and B were sectioned as indicated by the red line and sections are shown in A′ and B′, respectively. Note Spry1 and Spry2 expression in the ectoderm (ecto), mesoderm (meso) and endoderm (endo) at E8.5. Ngn2 expression is shown to indicate the position of the epibranchial placodes at E9 (E) and E9.5 (H) for comparison. The first (PA1), second (PA2) and third (PA3) pharyngeal arches; otic vesicle (OV); geniculate (G), petrosal (P) and nodose (N) placodes are labelled.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC3368431&req=5

f0005: Dynamic Spry1 and Spry2 expression in the developing pharyngeal region. Sprouty gene expression analysed by RNA in situ hybridisation. Spry1 and Spry2 expression in whole mount E8.5 (8–9ss) (A,B), E9 (16–20ss) (C,D) and E9.5 (25–26ss) (F,G) embryos, anterior to the top and ventral to the right. Embryos in A and B were sectioned as indicated by the red line and sections are shown in A′ and B′, respectively. Note Spry1 and Spry2 expression in the ectoderm (ecto), mesoderm (meso) and endoderm (endo) at E8.5. Ngn2 expression is shown to indicate the position of the epibranchial placodes at E9 (E) and E9.5 (H) for comparison. The first (PA1), second (PA2) and third (PA3) pharyngeal arches; otic vesicle (OV); geniculate (G), petrosal (P) and nodose (N) placodes are labelled.
Mentions: To determine whether Sprouty genes may have a function in the development of the epibranchial ganglia in the mouse, we analysed the expression of Spry1 and Spry2 between embryonic day (E)8.5 and E9.5 of development. At E8.5, Spry1 and Spry2 are both expressed in the pharyngeal ectoderm, endoderm and mesoderm (Figs. 1A–B′). This pattern of expression is consistent with the expression of several Fgf genes in these tissues at this time of development and the role of FGF signalling in the induction of otic fate (Ladher et al., 2010).

Bottom Line: Fibroblast growth factor (FGF) signalling has important roles in the development of the embryonic pharyngeal (branchial) arches, but its effects on innervation of the arches and associated structures have not been studied extensively.However, epithelial-specific gene deletion only results in defects in the facial nerve and not the glossopharyngeal and vagus nerves, suggesting that the facial nerve is most sensitive to perturbations in RTK signalling.Reducing the Fgf8 gene dosage only partially rescued defects in the glossopharyngeal nerve and was not sufficient to rescue facial nerve defects, suggesting that FGF8 is functionally redundant with other RTK ligands during facial nerve development.

View Article: PubMed Central - PubMed

Affiliation: Department of Craniofacial Development, King's College London, Floor 27, Guy's Tower, London, SE1 9RT, UK.

Show MeSH
Related in: MedlinePlus