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Complete Genome Sequence of a thermotolerant sporogenic lactic acid bacterium, Bacillus coagulans strain 36D1.

Rhee MS, Moritz BE, Xie G, Glavina Del Rio T, Dalin E, Tice H, Bruce D, Goodwin L, Chertkov O, Brettin T, Han C, Detter C, Pitluck S, Land ML, Patel M, Ou M, Harbrucker R, Ingram LO, Shanmugam KT - Stand Genomic Sci (2011)

Bottom Line: Bacillus coagulans is a ubiquitous soil bacterium that grows at 50-55 °C and pH 5.0 and ferments various sugars that constitute plant biomass to L (+)-lactic acid.This bacterium is also considered as a potential probiotic.Complete genome sequence of a representative strain, B. coagulans strain 36D1, is presented and discussed.

View Article: PubMed Central - PubMed

ABSTRACT
Bacillus coagulans is a ubiquitous soil bacterium that grows at 50-55 °C and pH 5.0 and ferments various sugars that constitute plant biomass to L (+)-lactic acid. The ability of this sporogenic lactic acid bacterium to grow at 50-55 °C and pH 5.0 makes this organism an attractive microbial biocatalyst for production of optically pure lactic acid at industrial scale not only from glucose derived from cellulose but also from xylose, a major constituent of hemicellulose. This bacterium is also considered as a potential probiotic. Complete genome sequence of a representative strain, B. coagulans strain 36D1, is presented and discussed.

No MeSH data available.


Related in: MedlinePlus

Thin section electron micrographs of B. coagulans strain 36D1. Left: vegetative cell. Upper right: sporulating cell. Lower right: mature spore.
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f1: Thin section electron micrographs of B. coagulans strain 36D1. Left: vegetative cell. Upper right: sporulating cell. Lower right: mature spore.

Mentions: B. coagulans was first isolated from coagulated milk by Hammer in 1915 [19]. Since then, several members of this group have been isolated from various sources [12,14]. B. coagulans strain 36D1 used in this study was isolated from a mud sample from an effluent stream of Old Faithful Geyser 1 near Calistoga, California, USA as an organism that can grow on xylose at 50°C and pH 5.0 both aerobically and anaerobically [14]. This bacterium is rod-shaped and produces endospore when cultured in nutrient broth (Fig. 1). Endospores are rarely observed when the bacterium was cultured in L-broth. Optimum temperature and pH for growth of strain 36D1 is 55°C and 5.5, respectively [10]. Corn steep liquor at 0.5% (w/v) provided the needed nutritional supplements for growth in mineral salts medium and the growth rate of the bacterium in that medium at 55°C was 1.67 h-1. The main fermentation product of the bacterium is L-lactate. Pentose fermentation increases the level of acetate, ethanol and formate in the medium compared to hexose fermentation [14]. Anaerobic cultures started with sparging of the medium with N2 require CO2 for growth. Other characteristics of the bacterium are listed in Table 1. B. coagulans strain 36D1 is deposited in the American Type Culture Collection (PTA-5827).


Complete Genome Sequence of a thermotolerant sporogenic lactic acid bacterium, Bacillus coagulans strain 36D1.

Rhee MS, Moritz BE, Xie G, Glavina Del Rio T, Dalin E, Tice H, Bruce D, Goodwin L, Chertkov O, Brettin T, Han C, Detter C, Pitluck S, Land ML, Patel M, Ou M, Harbrucker R, Ingram LO, Shanmugam KT - Stand Genomic Sci (2011)

Thin section electron micrographs of B. coagulans strain 36D1. Left: vegetative cell. Upper right: sporulating cell. Lower right: mature spore.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3368420&req=5

f1: Thin section electron micrographs of B. coagulans strain 36D1. Left: vegetative cell. Upper right: sporulating cell. Lower right: mature spore.
Mentions: B. coagulans was first isolated from coagulated milk by Hammer in 1915 [19]. Since then, several members of this group have been isolated from various sources [12,14]. B. coagulans strain 36D1 used in this study was isolated from a mud sample from an effluent stream of Old Faithful Geyser 1 near Calistoga, California, USA as an organism that can grow on xylose at 50°C and pH 5.0 both aerobically and anaerobically [14]. This bacterium is rod-shaped and produces endospore when cultured in nutrient broth (Fig. 1). Endospores are rarely observed when the bacterium was cultured in L-broth. Optimum temperature and pH for growth of strain 36D1 is 55°C and 5.5, respectively [10]. Corn steep liquor at 0.5% (w/v) provided the needed nutritional supplements for growth in mineral salts medium and the growth rate of the bacterium in that medium at 55°C was 1.67 h-1. The main fermentation product of the bacterium is L-lactate. Pentose fermentation increases the level of acetate, ethanol and formate in the medium compared to hexose fermentation [14]. Anaerobic cultures started with sparging of the medium with N2 require CO2 for growth. Other characteristics of the bacterium are listed in Table 1. B. coagulans strain 36D1 is deposited in the American Type Culture Collection (PTA-5827).

Bottom Line: Bacillus coagulans is a ubiquitous soil bacterium that grows at 50-55 °C and pH 5.0 and ferments various sugars that constitute plant biomass to L (+)-lactic acid.This bacterium is also considered as a potential probiotic.Complete genome sequence of a representative strain, B. coagulans strain 36D1, is presented and discussed.

View Article: PubMed Central - PubMed

ABSTRACT
Bacillus coagulans is a ubiquitous soil bacterium that grows at 50-55 °C and pH 5.0 and ferments various sugars that constitute plant biomass to L (+)-lactic acid. The ability of this sporogenic lactic acid bacterium to grow at 50-55 °C and pH 5.0 makes this organism an attractive microbial biocatalyst for production of optically pure lactic acid at industrial scale not only from glucose derived from cellulose but also from xylose, a major constituent of hemicellulose. This bacterium is also considered as a potential probiotic. Complete genome sequence of a representative strain, B. coagulans strain 36D1, is presented and discussed.

No MeSH data available.


Related in: MedlinePlus