High quality draft genome sequence of Segniliparus rugosus CDC 945(T)= (ATCC BAA-974(T)).
Bottom Line: A unique and interesting feature of this family is the presence of extremely long carbon-chain length mycolic acids bound in the cell wall.This report represents the second species in the genus to have its genome sequenced.The 3,567,567 bp long genome with 3,516 protein-coding and 49 RNA genes is part of the NIH Roadmap for Medical Research, Human Microbiome Project.
Segniliparus rugosus represents one of two species in the genus Segniliparus, the sole genus in the family Segniliparaceae. A unique and interesting feature of this family is the presence of extremely long carbon-chain length mycolic acids bound in the cell wall. S. rugosus is also a medically important species because it is an opportunistic pathogen associated with mammalian lung disease. This report represents the second species in the genus to have its genome sequenced. The 3,567,567 bp long genome with 3,516 protein-coding and 49 RNA genes is part of the NIH Roadmap for Medical Research, Human Microbiome Project.
No MeSH data available.
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Mentions: The cells of CDC 945T are irregular rods ranging in length and width from 0.55-0.90 µm by 1.9-4.5 µm (Table 1 and Figure 1). Colonies are wrinkled, rough and form in less than 7 days on 7H10 and 7H11 agar at an optimal temperature of 33oC . CDC 945T is aerobic, non-motile, asporogenous, and stains bright red with acid alcohol stain . It is mesophilic and demonstrates a temperature range for growth between 22 and 42 oC . Colonies grown for < 4 weeks are non-pigmented, nonphotochromogenic and do not produce a diagnostic odor . They do not produce aerial mycelium, spores or demonstrate true branching. Young colonies are creamy and smear easily when disturbed. Cell growth at ~4 weeks on Löwenstein-Jensen (LJ) medium produces a diffusible pink color in the agar at the leading edges of mature growth. Aged colonies on LJ develop a light buff pigment and demonstrate ‘greening’ from uptake of malachite green . CDC 945T is weakly positive for arylsulfatase at 7 days but is strongly positive in 14 days. No growth on MacConkey agar not containing crystal violet. CDC 945T grows in the presence of 5% sodium chloride at 7 days, in lysozyme at 21 days. Positive for iron uptake, nitrate reduction, tellurite reduction and tween opacity. Negative for tween hydrolysis .
No MeSH data available.