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Complete genome sequence of Thioalkalivibrio sp. K90mix.

Muyzer G, Sorokin DY, Mavromatis K, Lapidus A, Foster B, Sun H, Ivanova N, Pati A, D'haeseleer P, Woyke T, Kyrpides NC - Stand Genomic Sci (2011)

Bottom Line: Thioalkalivibrio sp.The strain was isolated from a mixture of sediment samples obtained from different soda lakes located in the Kulunda Steppe (Altai, Russia) based on its extreme potassium carbonate tolerance as an enrichment method.The genome was sequenced within the Joint Genome Institute Community Sequencing Program, because of its relevance to the sustainable removal of sulfide from wastewater and gas streams.

View Article: PubMed Central - PubMed

ABSTRACT
Thioalkalivibrio sp. K90mix is an obligately chemolithoautotrophic, natronophilic sulfur-oxidizing bacterium (SOxB) belonging to the family Ectothiorhodospiraceae within the Gammaproteobacteria. The strain was isolated from a mixture of sediment samples obtained from different soda lakes located in the Kulunda Steppe (Altai, Russia) based on its extreme potassium carbonate tolerance as an enrichment method. Here we report the complete genome sequence of strain K90mix and its annotation. The genome was sequenced within the Joint Genome Institute Community Sequencing Program, because of its relevance to the sustainable removal of sulfide from wastewater and gas streams.

No MeSH data available.


Related in: MedlinePlus

Phylogeny and hydropathy of StbA from different microorganisms. The blue boxes indicate sequences belonging to the cyanobacteria. The dots on the branches are bootstrap values between 50% and 75% (open dots) or between 75% and 100% (closed dots) The scale bar indicated 10% sequence difference. The sequence of Thioalkalivibrio sp. K90mix is indicated in bold. Hydropathy profiles were made for the StbA protein sequences of Synechocystis sp. PCC6803 and Thioalkalivibrio sp. K90mix to indicate transmembrane spanning domains.
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f4: Phylogeny and hydropathy of StbA from different microorganisms. The blue boxes indicate sequences belonging to the cyanobacteria. The dots on the branches are bootstrap values between 50% and 75% (open dots) or between 75% and 100% (closed dots) The scale bar indicated 10% sequence difference. The sequence of Thioalkalivibrio sp. K90mix is indicated in bold. Hydropathy profiles were made for the StbA protein sequences of Synechocystis sp. PCC6803 and Thioalkalivibrio sp. K90mix to indicate transmembrane spanning domains.

Mentions: As mentioned before, [32] autotrophic growth at extremely high pH is a problem, because inorganic carbon is mainly present as carbonate (with bicarbonate as a minor fraction) at pH values above 10. This would demand active transport of bicarbonate into the cell. We found a gene related to stbA encoding a Na+/HCO3- symporter in the marine cyanobacterium Synechocystis sp. strain PCC 6803 [33]. Figure 4 shows a phylogenetic tree of different sequences related to StbA and the hydrophobicity profiles of StbA of Synechocystis sp. PCC 6803 and Thioalkalivibrio sp. K90mix. In addition, we have found genes for the large (TK90_0858) and small subunit (TK90_0859) of RuBisCO form 1Ac, and for the synthesis of α-carboxysomes (TK90_0860 – TK90_0866), including csoSCA encoding a carboxysome shell alpha-type carbonic anhydrase, which was also found in genomic analysis of “Thioalkalivibrio sulfidophilus” HL-EbGr7 [32].


Complete genome sequence of Thioalkalivibrio sp. K90mix.

Muyzer G, Sorokin DY, Mavromatis K, Lapidus A, Foster B, Sun H, Ivanova N, Pati A, D'haeseleer P, Woyke T, Kyrpides NC - Stand Genomic Sci (2011)

Phylogeny and hydropathy of StbA from different microorganisms. The blue boxes indicate sequences belonging to the cyanobacteria. The dots on the branches are bootstrap values between 50% and 75% (open dots) or between 75% and 100% (closed dots) The scale bar indicated 10% sequence difference. The sequence of Thioalkalivibrio sp. K90mix is indicated in bold. Hydropathy profiles were made for the StbA protein sequences of Synechocystis sp. PCC6803 and Thioalkalivibrio sp. K90mix to indicate transmembrane spanning domains.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3368412&req=5

f4: Phylogeny and hydropathy of StbA from different microorganisms. The blue boxes indicate sequences belonging to the cyanobacteria. The dots on the branches are bootstrap values between 50% and 75% (open dots) or between 75% and 100% (closed dots) The scale bar indicated 10% sequence difference. The sequence of Thioalkalivibrio sp. K90mix is indicated in bold. Hydropathy profiles were made for the StbA protein sequences of Synechocystis sp. PCC6803 and Thioalkalivibrio sp. K90mix to indicate transmembrane spanning domains.
Mentions: As mentioned before, [32] autotrophic growth at extremely high pH is a problem, because inorganic carbon is mainly present as carbonate (with bicarbonate as a minor fraction) at pH values above 10. This would demand active transport of bicarbonate into the cell. We found a gene related to stbA encoding a Na+/HCO3- symporter in the marine cyanobacterium Synechocystis sp. strain PCC 6803 [33]. Figure 4 shows a phylogenetic tree of different sequences related to StbA and the hydrophobicity profiles of StbA of Synechocystis sp. PCC 6803 and Thioalkalivibrio sp. K90mix. In addition, we have found genes for the large (TK90_0858) and small subunit (TK90_0859) of RuBisCO form 1Ac, and for the synthesis of α-carboxysomes (TK90_0860 – TK90_0866), including csoSCA encoding a carboxysome shell alpha-type carbonic anhydrase, which was also found in genomic analysis of “Thioalkalivibrio sulfidophilus” HL-EbGr7 [32].

Bottom Line: Thioalkalivibrio sp.The strain was isolated from a mixture of sediment samples obtained from different soda lakes located in the Kulunda Steppe (Altai, Russia) based on its extreme potassium carbonate tolerance as an enrichment method.The genome was sequenced within the Joint Genome Institute Community Sequencing Program, because of its relevance to the sustainable removal of sulfide from wastewater and gas streams.

View Article: PubMed Central - PubMed

ABSTRACT
Thioalkalivibrio sp. K90mix is an obligately chemolithoautotrophic, natronophilic sulfur-oxidizing bacterium (SOxB) belonging to the family Ectothiorhodospiraceae within the Gammaproteobacteria. The strain was isolated from a mixture of sediment samples obtained from different soda lakes located in the Kulunda Steppe (Altai, Russia) based on its extreme potassium carbonate tolerance as an enrichment method. Here we report the complete genome sequence of strain K90mix and its annotation. The genome was sequenced within the Joint Genome Institute Community Sequencing Program, because of its relevance to the sustainable removal of sulfide from wastewater and gas streams.

No MeSH data available.


Related in: MedlinePlus