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Complete genome sequence of the aerobic, heterotroph Marinithermus hydrothermalis type strain (T1(T)) from a deep-sea hydrothermal vent chimney.

Copeland A, Gu W, Yasawong M, Lapidus A, Lucas S, Deshpande S, Pagani I, Tapia R, Cheng JF, Goodwin LA, Pitluck S, Liolios K, Ivanova N, Mavromatis K, Mikhailova N, Pati A, Chen A, Palaniappan K, Land M, Pan C, Brambilla EM, Rohde M, Tindall BJ, Sikorski J, Göker M, Detter JC, Bristow J, Eisen JA, Markowitz V, Hugenholtz P, Kyrpides NC, Klenk HP, Woyke T - Stand Genomic Sci (2012)

Bottom Line: M. hydrothermalis T1(T) is of interest because it may provide a new insight into the ecological significance of the aerobic, thermophilic decomposers in the circulation of organic compounds in deep-sea hydrothermal vent ecosystems.This is the first completed genome sequence of a member of the genus Marinithermus and the seventh sequence from the family Thermaceae.Here we describe the features of this organism, together with the complete genome sequence and annotation.

View Article: PubMed Central - PubMed

ABSTRACT
Marinithermus hydrothermalis Sako et al. 2003 is the type species of the monotypic genus Marinithermus. M. hydrothermalis T1(T) was the first isolate within the phylum "Thermus-Deinococcus" to exhibit optimal growth under a salinity equivalent to that of sea water and to have an absolute requirement for NaCl for growth. M. hydrothermalis T1(T) is of interest because it may provide a new insight into the ecological significance of the aerobic, thermophilic decomposers in the circulation of organic compounds in deep-sea hydrothermal vent ecosystems. This is the first completed genome sequence of a member of the genus Marinithermus and the seventh sequence from the family Thermaceae. Here we describe the features of this organism, together with the complete genome sequence and annotation. The 2,269,167 bp long genome with its 2,251 protein-coding and 59 RNA genes is a part of the Genomic Encyclopedia of Bacteria and Archaea project.

No MeSH data available.


Scanning electron micrograph of M. hydrothermalis T1T
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f2: Scanning electron micrograph of M. hydrothermalis T1T

Mentions: The cells of strain T1T are Gram-negative, non-motile, straight rods measuring 7.5 - 9.4 µm by 0.9 - 1.0 µm during the exponential growth phase [1] (Figure 2 and Table 1). In the stationary growth phase the cells tend to form filaments [1]. Rotund bodies were not observed from the cells [1]. Cells of strain T1T have an envelope which consists of a cytoplasmic membrane with a simple outline and a cell wall with an inner, electron-dense thin layer, which presumably represents the peptidoglycan [1]. Colonies are whitish and have 2.5 - 3.0 mm of diameter [1]. The organism is an obligate heterotroph and grows only under strictly aerobic culture conditions [1]. Growth was not observed in anaerobic or autotrophic culture conditions [1]. However, it should be noted that according to Mori and colleagues [32] this was tested only in the presence of sulfide. Steinsbu and colleagues [3] argue that it is therefore possible that M. hydrothermalis has the capability of anaerobic growth under unreduced conditions, as has been observed for Rhabdothermus arcticus, Vulcanithermus mediatlanticus, O. profundus and O. desulfurans [3,32-34]. Unlike members of the genus Thermus, reactions were negative for catalase- and cytochrome oxidase and hydrolysis of gelatin, starch or casein was negative [1]. Growth occurs over the temperature range of 50.0 - 72.5°C (optimum 67.5°C), pH range 6.25 - 7.75 (optimum pH 7.0), and at NaCl concentrations in the range 0.5 - 4.5% (optimum 3%) [1]. The generation time under the above listed optimal condition and in medium MJYPV is about 30 minutes [1]. M. hydrothermalis T1T differs from the members of the genera Oceanithermus by having a higher optimal temperature for growth and a higher oxygen tolerance [3]. Strain T1T is able to utilize complex organic substrates such as Casamino acids, tryptone and yeast extract as sole energy and carbon sources [1].


Complete genome sequence of the aerobic, heterotroph Marinithermus hydrothermalis type strain (T1(T)) from a deep-sea hydrothermal vent chimney.

Copeland A, Gu W, Yasawong M, Lapidus A, Lucas S, Deshpande S, Pagani I, Tapia R, Cheng JF, Goodwin LA, Pitluck S, Liolios K, Ivanova N, Mavromatis K, Mikhailova N, Pati A, Chen A, Palaniappan K, Land M, Pan C, Brambilla EM, Rohde M, Tindall BJ, Sikorski J, Göker M, Detter JC, Bristow J, Eisen JA, Markowitz V, Hugenholtz P, Kyrpides NC, Klenk HP, Woyke T - Stand Genomic Sci (2012)

Scanning electron micrograph of M. hydrothermalis T1T
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3368408&req=5

f2: Scanning electron micrograph of M. hydrothermalis T1T
Mentions: The cells of strain T1T are Gram-negative, non-motile, straight rods measuring 7.5 - 9.4 µm by 0.9 - 1.0 µm during the exponential growth phase [1] (Figure 2 and Table 1). In the stationary growth phase the cells tend to form filaments [1]. Rotund bodies were not observed from the cells [1]. Cells of strain T1T have an envelope which consists of a cytoplasmic membrane with a simple outline and a cell wall with an inner, electron-dense thin layer, which presumably represents the peptidoglycan [1]. Colonies are whitish and have 2.5 - 3.0 mm of diameter [1]. The organism is an obligate heterotroph and grows only under strictly aerobic culture conditions [1]. Growth was not observed in anaerobic or autotrophic culture conditions [1]. However, it should be noted that according to Mori and colleagues [32] this was tested only in the presence of sulfide. Steinsbu and colleagues [3] argue that it is therefore possible that M. hydrothermalis has the capability of anaerobic growth under unreduced conditions, as has been observed for Rhabdothermus arcticus, Vulcanithermus mediatlanticus, O. profundus and O. desulfurans [3,32-34]. Unlike members of the genus Thermus, reactions were negative for catalase- and cytochrome oxidase and hydrolysis of gelatin, starch or casein was negative [1]. Growth occurs over the temperature range of 50.0 - 72.5°C (optimum 67.5°C), pH range 6.25 - 7.75 (optimum pH 7.0), and at NaCl concentrations in the range 0.5 - 4.5% (optimum 3%) [1]. The generation time under the above listed optimal condition and in medium MJYPV is about 30 minutes [1]. M. hydrothermalis T1T differs from the members of the genera Oceanithermus by having a higher optimal temperature for growth and a higher oxygen tolerance [3]. Strain T1T is able to utilize complex organic substrates such as Casamino acids, tryptone and yeast extract as sole energy and carbon sources [1].

Bottom Line: M. hydrothermalis T1(T) is of interest because it may provide a new insight into the ecological significance of the aerobic, thermophilic decomposers in the circulation of organic compounds in deep-sea hydrothermal vent ecosystems.This is the first completed genome sequence of a member of the genus Marinithermus and the seventh sequence from the family Thermaceae.Here we describe the features of this organism, together with the complete genome sequence and annotation.

View Article: PubMed Central - PubMed

ABSTRACT
Marinithermus hydrothermalis Sako et al. 2003 is the type species of the monotypic genus Marinithermus. M. hydrothermalis T1(T) was the first isolate within the phylum "Thermus-Deinococcus" to exhibit optimal growth under a salinity equivalent to that of sea water and to have an absolute requirement for NaCl for growth. M. hydrothermalis T1(T) is of interest because it may provide a new insight into the ecological significance of the aerobic, thermophilic decomposers in the circulation of organic compounds in deep-sea hydrothermal vent ecosystems. This is the first completed genome sequence of a member of the genus Marinithermus and the seventh sequence from the family Thermaceae. Here we describe the features of this organism, together with the complete genome sequence and annotation. The 2,269,167 bp long genome with its 2,251 protein-coding and 59 RNA genes is a part of the Genomic Encyclopedia of Bacteria and Archaea project.

No MeSH data available.