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Correlations between Endothelial Functions and ROS Detection in Diabetic Microvascular Wall: Early and Late Ascorbic Acid Supplementation.

Sridulyakul P, Wongeak-In N, Patumraj S - Int J Vasc Med (2012)

Bottom Line: On 12th week after STZ injection, the findings showed that in DM group, Ach (10(-5) M)-induced vasodilatation was decreased, while the number of leukocyte adhesion was increased significantly (P < 0.01).By using dihydrorhodamine 123, our findings also indicated that the existing of ROS productions on diabetic arteriolar and venular walls were different significantly (ROS(arteriole) = 165.89 ± 24.59 and ROS(venule) = 172.26 ± 34.70) (P < 0.05).Moreover by using BH4 inhibitor to induce increase in arteriolar ROS, the results also confirmed that AA could improve endothelial function with closed correlation to its potential to reduce vascular ROS content.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Faculty of Science, Srinakharinwirot University, Bangkok 10110, Thailand.

ABSTRACT
The correlation between endothelial function and reactive oxygen species detecting from diabetic microvascular wall and the antioxidant effect of ascorbic acid (AA) during early and late phases of diabetic induction were determined. Male Spraque-Dawley rats were divided into four groups: control, diabetes rats (DM, using iv.injection of 55 mg/kg BW streptozotocin, (STZ)), and two groups of DM rats treated with AA (1 g/L, (STZ)) starting on day 2 (DM + AAday2) and week 6th (DM + AA6wk). On 12th week after STZ injection, the findings showed that in DM group, Ach (10(-5) M)-induced vasodilatation was decreased, while the number of leukocyte adhesion was increased significantly (P < 0.01). Interestingly, these abnormalities induced by DM could be protected or improved in both AA-treated groups, DM + AAday2 and DM + AA6wk. By using dihydrorhodamine 123, our findings also indicated that the existing of ROS productions on diabetic arteriolar and venular walls were different significantly (ROS(arteriole) = 165.89 ± 24.59 and ROS(venule) = 172.26 ± 34.70) (P < 0.05). Moreover by using BH4 inhibitor to induce increase in arteriolar ROS, the results also confirmed that AA could improve endothelial function with closed correlation to its potential to reduce vascular ROS content.

No MeSH data available.


Related in: MedlinePlus

The correlation and regression line between percentage changes of ROS (intensities/100-μm arteriolar length) and arteriolar diameters in response to Ach from mesenteric arterioles in the presence or absence of BH4 synthetic inhibitor were obtained when combined the data of every group.
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fig5: The correlation and regression line between percentage changes of ROS (intensities/100-μm arteriolar length) and arteriolar diameters in response to Ach from mesenteric arterioles in the presence or absence of BH4 synthetic inhibitor were obtained when combined the data of every group.

Mentions: The results showed in Table 3 indicated that the ROS-dependent fluorescence intensity was significantly increased when BH4 synthesis was blocked by DAHP in relation to CON + Veh group (DAHP-treated CON = 169.61 ± 6.46% and CON + Veh = 54.48 ± 14.90%, resp.). Whereas the enhanced ROS content was significantly decreased in AA and 6R-BH4 treated groups (CON + DAHP + AA + BH4 = 33.89 ± 7.62%, resp.). Moreover, AA in combination with BH4-treated group showed synergistic increase in Ach-induced vasodilation when compared to CON + DAHP. By using Pearson correlation and linear regression analysis, the relationship between ROS-dependent fluorescence intensity and Ach-response can be described by the linear equation: y = −0.097x + 23.45; R2 = 0.61, as shown in Figure 5.


Correlations between Endothelial Functions and ROS Detection in Diabetic Microvascular Wall: Early and Late Ascorbic Acid Supplementation.

Sridulyakul P, Wongeak-In N, Patumraj S - Int J Vasc Med (2012)

The correlation and regression line between percentage changes of ROS (intensities/100-μm arteriolar length) and arteriolar diameters in response to Ach from mesenteric arterioles in the presence or absence of BH4 synthetic inhibitor were obtained when combined the data of every group.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3368388&req=5

fig5: The correlation and regression line between percentage changes of ROS (intensities/100-μm arteriolar length) and arteriolar diameters in response to Ach from mesenteric arterioles in the presence or absence of BH4 synthetic inhibitor were obtained when combined the data of every group.
Mentions: The results showed in Table 3 indicated that the ROS-dependent fluorescence intensity was significantly increased when BH4 synthesis was blocked by DAHP in relation to CON + Veh group (DAHP-treated CON = 169.61 ± 6.46% and CON + Veh = 54.48 ± 14.90%, resp.). Whereas the enhanced ROS content was significantly decreased in AA and 6R-BH4 treated groups (CON + DAHP + AA + BH4 = 33.89 ± 7.62%, resp.). Moreover, AA in combination with BH4-treated group showed synergistic increase in Ach-induced vasodilation when compared to CON + DAHP. By using Pearson correlation and linear regression analysis, the relationship between ROS-dependent fluorescence intensity and Ach-response can be described by the linear equation: y = −0.097x + 23.45; R2 = 0.61, as shown in Figure 5.

Bottom Line: On 12th week after STZ injection, the findings showed that in DM group, Ach (10(-5) M)-induced vasodilatation was decreased, while the number of leukocyte adhesion was increased significantly (P < 0.01).By using dihydrorhodamine 123, our findings also indicated that the existing of ROS productions on diabetic arteriolar and venular walls were different significantly (ROS(arteriole) = 165.89 ± 24.59 and ROS(venule) = 172.26 ± 34.70) (P < 0.05).Moreover by using BH4 inhibitor to induce increase in arteriolar ROS, the results also confirmed that AA could improve endothelial function with closed correlation to its potential to reduce vascular ROS content.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Faculty of Science, Srinakharinwirot University, Bangkok 10110, Thailand.

ABSTRACT
The correlation between endothelial function and reactive oxygen species detecting from diabetic microvascular wall and the antioxidant effect of ascorbic acid (AA) during early and late phases of diabetic induction were determined. Male Spraque-Dawley rats were divided into four groups: control, diabetes rats (DM, using iv.injection of 55 mg/kg BW streptozotocin, (STZ)), and two groups of DM rats treated with AA (1 g/L, (STZ)) starting on day 2 (DM + AAday2) and week 6th (DM + AA6wk). On 12th week after STZ injection, the findings showed that in DM group, Ach (10(-5) M)-induced vasodilatation was decreased, while the number of leukocyte adhesion was increased significantly (P < 0.01). Interestingly, these abnormalities induced by DM could be protected or improved in both AA-treated groups, DM + AAday2 and DM + AA6wk. By using dihydrorhodamine 123, our findings also indicated that the existing of ROS productions on diabetic arteriolar and venular walls were different significantly (ROS(arteriole) = 165.89 ± 24.59 and ROS(venule) = 172.26 ± 34.70) (P < 0.05). Moreover by using BH4 inhibitor to induce increase in arteriolar ROS, the results also confirmed that AA could improve endothelial function with closed correlation to its potential to reduce vascular ROS content.

No MeSH data available.


Related in: MedlinePlus