Limits...
Correlations between Endothelial Functions and ROS Detection in Diabetic Microvascular Wall: Early and Late Ascorbic Acid Supplementation.

Sridulyakul P, Wongeak-In N, Patumraj S - Int J Vasc Med (2012)

Bottom Line: On 12th week after STZ injection, the findings showed that in DM group, Ach (10(-5) M)-induced vasodilatation was decreased, while the number of leukocyte adhesion was increased significantly (P < 0.01).By using dihydrorhodamine 123, our findings also indicated that the existing of ROS productions on diabetic arteriolar and venular walls were different significantly (ROS(arteriole) = 165.89 ± 24.59 and ROS(venule) = 172.26 ± 34.70) (P < 0.05).Moreover by using BH4 inhibitor to induce increase in arteriolar ROS, the results also confirmed that AA could improve endothelial function with closed correlation to its potential to reduce vascular ROS content.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Faculty of Science, Srinakharinwirot University, Bangkok 10110, Thailand.

ABSTRACT
The correlation between endothelial function and reactive oxygen species detecting from diabetic microvascular wall and the antioxidant effect of ascorbic acid (AA) during early and late phases of diabetic induction were determined. Male Spraque-Dawley rats were divided into four groups: control, diabetes rats (DM, using iv.injection of 55 mg/kg BW streptozotocin, (STZ)), and two groups of DM rats treated with AA (1 g/L, (STZ)) starting on day 2 (DM + AAday2) and week 6th (DM + AA6wk). On 12th week after STZ injection, the findings showed that in DM group, Ach (10(-5) M)-induced vasodilatation was decreased, while the number of leukocyte adhesion was increased significantly (P < 0.01). Interestingly, these abnormalities induced by DM could be protected or improved in both AA-treated groups, DM + AAday2 and DM + AA6wk. By using dihydrorhodamine 123, our findings also indicated that the existing of ROS productions on diabetic arteriolar and venular walls were different significantly (ROS(arteriole) = 165.89 ± 24.59 and ROS(venule) = 172.26 ± 34.70) (P < 0.05). Moreover by using BH4 inhibitor to induce increase in arteriolar ROS, the results also confirmed that AA could improve endothelial function with closed correlation to its potential to reduce vascular ROS content.

No MeSH data available.


Related in: MedlinePlus

Percentage of changed DHR intensities and number (Cn) of leukocyte-endothelial cell interaction in each group of control (CON), diabetes (DM), diabetes treated with vitamin C (1 g/L) starting on day 2 (DM + AAday2), and on 6 weeks (DM + AA6wk) after STZ injection was plotted. The correlation and regression line were obtained for each group as demonstrated in (a). In (b), the correlation and regression line were obtained for combined data of every group.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3368388&req=5

fig4: Percentage of changed DHR intensities and number (Cn) of leukocyte-endothelial cell interaction in each group of control (CON), diabetes (DM), diabetes treated with vitamin C (1 g/L) starting on day 2 (DM + AAday2), and on 6 weeks (DM + AA6wk) after STZ injection was plotted. The correlation and regression line were obtained for each group as demonstrated in (a). In (b), the correlation and regression line were obtained for combined data of every group.

Mentions: Figure 4 showed the changes in the ROS-dependent fluorescence intensities detected from the rat mesenteric arterioles and venules of each group at 0 and 1 minute after DHR-123 superfusion. It was observed that DM showed the strongly increased fluorescence intensity as early as 1 min after DHR-123 superfusion. The changes in fluorescence intensities from CON, DM, DM + AAday2, and DM + AA6wk groups after 1-min DHR superfusion were analyzed using digital image analysis as described in methodology above. The averages ROS contents determined from both vascular walls of each group (CON, DM, DM + AAday2, and DM + AA6wk) were summarized in Table 2. These findings indicated that early AA supplementation as well as delayed supplementation of AA could similarly attenuate the increase in ROS generation at both sites of microvascular walls. Interestingly, our findings also indicated that the existing of ROS productions on diabetic arteriolar and venular walls (Table 2) were ROSarteriole = 165.89 ± 24.59 and ROSvenule = 172.26 ± 34.70. (P < 0.05).


Correlations between Endothelial Functions and ROS Detection in Diabetic Microvascular Wall: Early and Late Ascorbic Acid Supplementation.

Sridulyakul P, Wongeak-In N, Patumraj S - Int J Vasc Med (2012)

Percentage of changed DHR intensities and number (Cn) of leukocyte-endothelial cell interaction in each group of control (CON), diabetes (DM), diabetes treated with vitamin C (1 g/L) starting on day 2 (DM + AAday2), and on 6 weeks (DM + AA6wk) after STZ injection was plotted. The correlation and regression line were obtained for each group as demonstrated in (a). In (b), the correlation and regression line were obtained for combined data of every group.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3368388&req=5

fig4: Percentage of changed DHR intensities and number (Cn) of leukocyte-endothelial cell interaction in each group of control (CON), diabetes (DM), diabetes treated with vitamin C (1 g/L) starting on day 2 (DM + AAday2), and on 6 weeks (DM + AA6wk) after STZ injection was plotted. The correlation and regression line were obtained for each group as demonstrated in (a). In (b), the correlation and regression line were obtained for combined data of every group.
Mentions: Figure 4 showed the changes in the ROS-dependent fluorescence intensities detected from the rat mesenteric arterioles and venules of each group at 0 and 1 minute after DHR-123 superfusion. It was observed that DM showed the strongly increased fluorescence intensity as early as 1 min after DHR-123 superfusion. The changes in fluorescence intensities from CON, DM, DM + AAday2, and DM + AA6wk groups after 1-min DHR superfusion were analyzed using digital image analysis as described in methodology above. The averages ROS contents determined from both vascular walls of each group (CON, DM, DM + AAday2, and DM + AA6wk) were summarized in Table 2. These findings indicated that early AA supplementation as well as delayed supplementation of AA could similarly attenuate the increase in ROS generation at both sites of microvascular walls. Interestingly, our findings also indicated that the existing of ROS productions on diabetic arteriolar and venular walls (Table 2) were ROSarteriole = 165.89 ± 24.59 and ROSvenule = 172.26 ± 34.70. (P < 0.05).

Bottom Line: On 12th week after STZ injection, the findings showed that in DM group, Ach (10(-5) M)-induced vasodilatation was decreased, while the number of leukocyte adhesion was increased significantly (P < 0.01).By using dihydrorhodamine 123, our findings also indicated that the existing of ROS productions on diabetic arteriolar and venular walls were different significantly (ROS(arteriole) = 165.89 ± 24.59 and ROS(venule) = 172.26 ± 34.70) (P < 0.05).Moreover by using BH4 inhibitor to induce increase in arteriolar ROS, the results also confirmed that AA could improve endothelial function with closed correlation to its potential to reduce vascular ROS content.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Faculty of Science, Srinakharinwirot University, Bangkok 10110, Thailand.

ABSTRACT
The correlation between endothelial function and reactive oxygen species detecting from diabetic microvascular wall and the antioxidant effect of ascorbic acid (AA) during early and late phases of diabetic induction were determined. Male Spraque-Dawley rats were divided into four groups: control, diabetes rats (DM, using iv.injection of 55 mg/kg BW streptozotocin, (STZ)), and two groups of DM rats treated with AA (1 g/L, (STZ)) starting on day 2 (DM + AAday2) and week 6th (DM + AA6wk). On 12th week after STZ injection, the findings showed that in DM group, Ach (10(-5) M)-induced vasodilatation was decreased, while the number of leukocyte adhesion was increased significantly (P < 0.01). Interestingly, these abnormalities induced by DM could be protected or improved in both AA-treated groups, DM + AAday2 and DM + AA6wk. By using dihydrorhodamine 123, our findings also indicated that the existing of ROS productions on diabetic arteriolar and venular walls were different significantly (ROS(arteriole) = 165.89 ± 24.59 and ROS(venule) = 172.26 ± 34.70) (P < 0.05). Moreover by using BH4 inhibitor to induce increase in arteriolar ROS, the results also confirmed that AA could improve endothelial function with closed correlation to its potential to reduce vascular ROS content.

No MeSH data available.


Related in: MedlinePlus