Limits...
Correlations between Endothelial Functions and ROS Detection in Diabetic Microvascular Wall: Early and Late Ascorbic Acid Supplementation.

Sridulyakul P, Wongeak-In N, Patumraj S - Int J Vasc Med (2012)

Bottom Line: On 12th week after STZ injection, the findings showed that in DM group, Ach (10(-5) M)-induced vasodilatation was decreased, while the number of leukocyte adhesion was increased significantly (P < 0.01).By using dihydrorhodamine 123, our findings also indicated that the existing of ROS productions on diabetic arteriolar and venular walls were different significantly (ROS(arteriole) = 165.89 ± 24.59 and ROS(venule) = 172.26 ± 34.70) (P < 0.05).Moreover by using BH4 inhibitor to induce increase in arteriolar ROS, the results also confirmed that AA could improve endothelial function with closed correlation to its potential to reduce vascular ROS content.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Faculty of Science, Srinakharinwirot University, Bangkok 10110, Thailand.

ABSTRACT
The correlation between endothelial function and reactive oxygen species detecting from diabetic microvascular wall and the antioxidant effect of ascorbic acid (AA) during early and late phases of diabetic induction were determined. Male Spraque-Dawley rats were divided into four groups: control, diabetes rats (DM, using iv.injection of 55 mg/kg BW streptozotocin, (STZ)), and two groups of DM rats treated with AA (1 g/L, (STZ)) starting on day 2 (DM + AAday2) and week 6th (DM + AA6wk). On 12th week after STZ injection, the findings showed that in DM group, Ach (10(-5) M)-induced vasodilatation was decreased, while the number of leukocyte adhesion was increased significantly (P < 0.01). Interestingly, these abnormalities induced by DM could be protected or improved in both AA-treated groups, DM + AAday2 and DM + AA6wk. By using dihydrorhodamine 123, our findings also indicated that the existing of ROS productions on diabetic arteriolar and venular walls were different significantly (ROS(arteriole) = 165.89 ± 24.59 and ROS(venule) = 172.26 ± 34.70) (P < 0.05). Moreover by using BH4 inhibitor to induce increase in arteriolar ROS, the results also confirmed that AA could improve endothelial function with closed correlation to its potential to reduce vascular ROS content.

No MeSH data available.


Related in: MedlinePlus

Number (Cn) of leukocyte adhesion in control (CON), diabetes (DM), diabetes treated with vitamin C (1g/L) starting on day 2 (DM + AAday2) and on 6 weeks (DM + AA6wk) after STZ injection. Values are mean ± SEM, CON (n = 8), DM (n = 6), DM + AAday2 (n = 7), and DM + AA6wk (n = 7). *P < 0.001 versus CON, #P < 0.01 versus DM.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3368388&req=5

fig3: Number (Cn) of leukocyte adhesion in control (CON), diabetes (DM), diabetes treated with vitamin C (1g/L) starting on day 2 (DM + AAday2) and on 6 weeks (DM + AA6wk) after STZ injection. Values are mean ± SEM, CON (n = 8), DM (n = 6), DM + AAday2 (n = 7), and DM + AA6wk (n = 7). *P < 0.001 versus CON, #P < 0.01 versus DM.

Mentions: Figure 3 showed the number of leukocyte adhesion in CON, DM, DM + AAday2 and DM + AA6wk groups. Their adherent-leukocyte densities were changed from 9.17 ± 1.45 cells/100 μm in DM group to 4.71 ± 0.43 cells/100 μm in DM + AAday2 group and to 2.15 ± 0.31 cells/100 μm in DM + AA6wk group, respectively. Interestingly, the number of leukocyte adhesion was decreased significantly by both intervals of AA supplementation.


Correlations between Endothelial Functions and ROS Detection in Diabetic Microvascular Wall: Early and Late Ascorbic Acid Supplementation.

Sridulyakul P, Wongeak-In N, Patumraj S - Int J Vasc Med (2012)

Number (Cn) of leukocyte adhesion in control (CON), diabetes (DM), diabetes treated with vitamin C (1g/L) starting on day 2 (DM + AAday2) and on 6 weeks (DM + AA6wk) after STZ injection. Values are mean ± SEM, CON (n = 8), DM (n = 6), DM + AAday2 (n = 7), and DM + AA6wk (n = 7). *P < 0.001 versus CON, #P < 0.01 versus DM.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3368388&req=5

fig3: Number (Cn) of leukocyte adhesion in control (CON), diabetes (DM), diabetes treated with vitamin C (1g/L) starting on day 2 (DM + AAday2) and on 6 weeks (DM + AA6wk) after STZ injection. Values are mean ± SEM, CON (n = 8), DM (n = 6), DM + AAday2 (n = 7), and DM + AA6wk (n = 7). *P < 0.001 versus CON, #P < 0.01 versus DM.
Mentions: Figure 3 showed the number of leukocyte adhesion in CON, DM, DM + AAday2 and DM + AA6wk groups. Their adherent-leukocyte densities were changed from 9.17 ± 1.45 cells/100 μm in DM group to 4.71 ± 0.43 cells/100 μm in DM + AAday2 group and to 2.15 ± 0.31 cells/100 μm in DM + AA6wk group, respectively. Interestingly, the number of leukocyte adhesion was decreased significantly by both intervals of AA supplementation.

Bottom Line: On 12th week after STZ injection, the findings showed that in DM group, Ach (10(-5) M)-induced vasodilatation was decreased, while the number of leukocyte adhesion was increased significantly (P < 0.01).By using dihydrorhodamine 123, our findings also indicated that the existing of ROS productions on diabetic arteriolar and venular walls were different significantly (ROS(arteriole) = 165.89 ± 24.59 and ROS(venule) = 172.26 ± 34.70) (P < 0.05).Moreover by using BH4 inhibitor to induce increase in arteriolar ROS, the results also confirmed that AA could improve endothelial function with closed correlation to its potential to reduce vascular ROS content.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Faculty of Science, Srinakharinwirot University, Bangkok 10110, Thailand.

ABSTRACT
The correlation between endothelial function and reactive oxygen species detecting from diabetic microvascular wall and the antioxidant effect of ascorbic acid (AA) during early and late phases of diabetic induction were determined. Male Spraque-Dawley rats were divided into four groups: control, diabetes rats (DM, using iv.injection of 55 mg/kg BW streptozotocin, (STZ)), and two groups of DM rats treated with AA (1 g/L, (STZ)) starting on day 2 (DM + AAday2) and week 6th (DM + AA6wk). On 12th week after STZ injection, the findings showed that in DM group, Ach (10(-5) M)-induced vasodilatation was decreased, while the number of leukocyte adhesion was increased significantly (P < 0.01). Interestingly, these abnormalities induced by DM could be protected or improved in both AA-treated groups, DM + AAday2 and DM + AA6wk. By using dihydrorhodamine 123, our findings also indicated that the existing of ROS productions on diabetic arteriolar and venular walls were different significantly (ROS(arteriole) = 165.89 ± 24.59 and ROS(venule) = 172.26 ± 34.70) (P < 0.05). Moreover by using BH4 inhibitor to induce increase in arteriolar ROS, the results also confirmed that AA could improve endothelial function with closed correlation to its potential to reduce vascular ROS content.

No MeSH data available.


Related in: MedlinePlus