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Anxiolytic-like effects of compound zhi zhu xiang in rats.

Wang YL, Shi JL, Yong L, Ren Z, Zhai YJ, Guo JY - Evid Based Complement Alternat Med (2012)

Bottom Line: In the VCT, repeated treatment with CZZX (1.5 and 3 g/kg/day, p.o.) significantly increased the number of punished licks.In the open field, locomotion was not reduced, discarding the possible sedative effect of CZZX.These data indicate an anxiolytic-like profile of action for CZZX without sedative side effects, and this activity may be mediated by benzodiazepine binding site modulation at γ-aminobutyric acid-A receptors.

View Article: PubMed Central - PubMed

Affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China.

ABSTRACT
The purpose of this study was to determine whether compound zhi zhu xiang (CZZX) exerts anxiolytic-like effects in rats. The animals were orally administered CZZX (0.75, 1.5, and 3 g/kg daily) for 10 days and tested in the elevated plus maze (EPM), Vogel conflict test (VCT), and open field. Repeated treatment with CZZX (3 g/kg/day, p.o.) significantly increased the percentage of both entries into and time spent on the open arms of the EPM compared with saline controls. In the VCT, repeated treatment with CZZX (1.5 and 3 g/kg/day, p.o.) significantly increased the number of punished licks. The drug did not change the total entries into the open arms of the EPM or interfere with water consumption or nociceptive threshold, discarding potential confounding factors in the two tests. In the open field, locomotion was not reduced, discarding the possible sedative effect of CZZX. In the binding assay, the binding of [(3)H] Ro 15-1788 (flumazenil) to the benzodiazepine binding site in washed crude synaptosomal membranes from rat cerebral cortex was affected by CZZX. These data indicate an anxiolytic-like profile of action for CZZX without sedative side effects, and this activity may be mediated by benzodiazepine binding site modulation at γ-aminobutyric acid-A receptors.

No MeSH data available.


Related in: MedlinePlus

Behavioural performance of rat registered in a 5 min session in the elevated plus maze performed 1 h after the injection of vehicle (VEH, p.o.) and CZZX (0.75, 1.5 and 3 g/kg, p.o.) or 0.5 h after the injection of diazepam (DZP, 1 mg/kg, p.o.). (a) Percentage of the number of entries into the open arm, (b) percentage of time spent into the open arms and (c) total arm entries. Columns represent the means ± SEM, n = 9-10 rats. *P < 0.05, **P < 0.01 compared to the control group.
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fig1: Behavioural performance of rat registered in a 5 min session in the elevated plus maze performed 1 h after the injection of vehicle (VEH, p.o.) and CZZX (0.75, 1.5 and 3 g/kg, p.o.) or 0.5 h after the injection of diazepam (DZP, 1 mg/kg, p.o.). (a) Percentage of the number of entries into the open arm, (b) percentage of time spent into the open arms and (c) total arm entries. Columns represent the means ± SEM, n = 9-10 rats. *P < 0.05, **P < 0.01 compared to the control group.

Mentions: The one-way ANOVA indicated significant differences among groups in the time spent in the open arms of the elevated plus maze (F4,44 = 6.72, P < 0.01; Figure 1(a)) and percentage of open arm entries (F4,44 = 5.08, P < 0.01; Figure 1(b)). CZZX at a dose of 3 g/kg produced anxiolytic-like effects, reflected by an increase in the percentage of open arm entries (P < 0.05). Doses of 1.5 and 3 g/kg also increased the time spent in the open arms of the maze (P < 0.05 and P < 0.01, resp.). No differences were observed in total arm entries (F4,44 = 0.88, P > 0.05; Figure 1(c)).


Anxiolytic-like effects of compound zhi zhu xiang in rats.

Wang YL, Shi JL, Yong L, Ren Z, Zhai YJ, Guo JY - Evid Based Complement Alternat Med (2012)

Behavioural performance of rat registered in a 5 min session in the elevated plus maze performed 1 h after the injection of vehicle (VEH, p.o.) and CZZX (0.75, 1.5 and 3 g/kg, p.o.) or 0.5 h after the injection of diazepam (DZP, 1 mg/kg, p.o.). (a) Percentage of the number of entries into the open arm, (b) percentage of time spent into the open arms and (c) total arm entries. Columns represent the means ± SEM, n = 9-10 rats. *P < 0.05, **P < 0.01 compared to the control group.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3368380&req=5

fig1: Behavioural performance of rat registered in a 5 min session in the elevated plus maze performed 1 h after the injection of vehicle (VEH, p.o.) and CZZX (0.75, 1.5 and 3 g/kg, p.o.) or 0.5 h after the injection of diazepam (DZP, 1 mg/kg, p.o.). (a) Percentage of the number of entries into the open arm, (b) percentage of time spent into the open arms and (c) total arm entries. Columns represent the means ± SEM, n = 9-10 rats. *P < 0.05, **P < 0.01 compared to the control group.
Mentions: The one-way ANOVA indicated significant differences among groups in the time spent in the open arms of the elevated plus maze (F4,44 = 6.72, P < 0.01; Figure 1(a)) and percentage of open arm entries (F4,44 = 5.08, P < 0.01; Figure 1(b)). CZZX at a dose of 3 g/kg produced anxiolytic-like effects, reflected by an increase in the percentage of open arm entries (P < 0.05). Doses of 1.5 and 3 g/kg also increased the time spent in the open arms of the maze (P < 0.05 and P < 0.01, resp.). No differences were observed in total arm entries (F4,44 = 0.88, P > 0.05; Figure 1(c)).

Bottom Line: In the VCT, repeated treatment with CZZX (1.5 and 3 g/kg/day, p.o.) significantly increased the number of punished licks.In the open field, locomotion was not reduced, discarding the possible sedative effect of CZZX.These data indicate an anxiolytic-like profile of action for CZZX without sedative side effects, and this activity may be mediated by benzodiazepine binding site modulation at γ-aminobutyric acid-A receptors.

View Article: PubMed Central - PubMed

Affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China.

ABSTRACT
The purpose of this study was to determine whether compound zhi zhu xiang (CZZX) exerts anxiolytic-like effects in rats. The animals were orally administered CZZX (0.75, 1.5, and 3 g/kg daily) for 10 days and tested in the elevated plus maze (EPM), Vogel conflict test (VCT), and open field. Repeated treatment with CZZX (3 g/kg/day, p.o.) significantly increased the percentage of both entries into and time spent on the open arms of the EPM compared with saline controls. In the VCT, repeated treatment with CZZX (1.5 and 3 g/kg/day, p.o.) significantly increased the number of punished licks. The drug did not change the total entries into the open arms of the EPM or interfere with water consumption or nociceptive threshold, discarding potential confounding factors in the two tests. In the open field, locomotion was not reduced, discarding the possible sedative effect of CZZX. In the binding assay, the binding of [(3)H] Ro 15-1788 (flumazenil) to the benzodiazepine binding site in washed crude synaptosomal membranes from rat cerebral cortex was affected by CZZX. These data indicate an anxiolytic-like profile of action for CZZX without sedative side effects, and this activity may be mediated by benzodiazepine binding site modulation at γ-aminobutyric acid-A receptors.

No MeSH data available.


Related in: MedlinePlus