Limits...
Treatment with Y-27632, a ROCK Inhibitor, Increases the Proinvasive Nature of SW620 Cells on 3D Collagen Type 1 Matrix.

Vishnubhotla R, Bharadwaj S, Sun S, Metlushko V, Glover SC - Int J Cell Biol (2012)

Bottom Line: Increasing collagen I scaffold density resulted in a 2.5-fold increase in cell proliferation while treatment with Y-27632 attenuated this effect although 1.5 fold increase in cell invasion was observed in ROCK inhibited samples.Intriguingly, ROCK inhibition also resulted in a 3.5-fold increase in cell invasion within 3D collagen scaffolds for cells seeded at lower densities.This data suggests that although ROCK inhibitors have been used clinically to treat several medical conditions, its effect largely depends on the surrounding microenvironment.

View Article: PubMed Central - PubMed

Affiliation: Department of Bioengineering, University of Illinois at Chicago, Chicago, IL 60612, USA.

ABSTRACT
The concept of using tissue density as a mechanism to diagnose a tumor has been around for centuries. However, this concept has not been sufficiently explored in a laboratory setting. Therefore, in this paper, we observed the effects of cell density and extracellular matrix (ECM) density on colon cancer invasion and proliferation using SW620 cells. We also attempted to inhibit ROCK-I to determine its effect on cell invasion and proliferation using standard molecular biology techniques and advanced imaging. Increasing cell seeding density resulted in a 2-fold increase in cell invasion as well as cell proliferation independent of treatment with Y-27632. Increasing collagen I scaffold density resulted in a 2.5-fold increase in cell proliferation while treatment with Y-27632 attenuated this effect although 1.5 fold increase in cell invasion was observed in ROCK inhibited samples. Intriguingly, ROCK inhibition also resulted in a 3.5-fold increase in cell invasion within 3D collagen scaffolds for cells seeded at lower densities. We show in this paper that ROCK-I inhibition leads to increased invasion within 3D collagen I microenvironments. This data suggests that although ROCK inhibitors have been used clinically to treat several medical conditions, its effect largely depends on the surrounding microenvironment.

No MeSH data available.


Related in: MedlinePlus

SW620 cells were seeded at densities of 50 × 103 and 250 × 103 cells/cm2 onto 1.5 mg/mL collagen I gels. Scaffolds were treated with Y-27632 to study the impact of ROCK inhibition on cell invasion in a low- and high-density environment. Treatment with Y-27632 resulted in a 3.5-fold increase in cell invasion for cells seeded at 50 × 103 cells/cm2.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3368365&req=5

fig4: SW620 cells were seeded at densities of 50 × 103 and 250 × 103 cells/cm2 onto 1.5 mg/mL collagen I gels. Scaffolds were treated with Y-27632 to study the impact of ROCK inhibition on cell invasion in a low- and high-density environment. Treatment with Y-27632 resulted in a 3.5-fold increase in cell invasion for cells seeded at 50 × 103 cells/cm2.

Mentions: Samples were tracked using a cell tracker dye. Seeding density did affect invasion depth of SW620 cells in collagen I scaffolds. Cells seeded at 250 × 103 cells/cm2 invaded twice the distance than those seeded at 50 × 103 cells/cm2 for untreated. However, this effect was not observed for SW620 cells treated with Y-27632. For cells seeded at the lower seeding density, treatment with Y-27632 led to a 3.5-fold increase in invasion depth. However, no significant increase was observed in cells seeded at 250 × 103 cells/cm2. Standard Student t-test was performed and a P value of 0.05 was considered significant (∗). The data is shown in Figure 4.


Treatment with Y-27632, a ROCK Inhibitor, Increases the Proinvasive Nature of SW620 Cells on 3D Collagen Type 1 Matrix.

Vishnubhotla R, Bharadwaj S, Sun S, Metlushko V, Glover SC - Int J Cell Biol (2012)

SW620 cells were seeded at densities of 50 × 103 and 250 × 103 cells/cm2 onto 1.5 mg/mL collagen I gels. Scaffolds were treated with Y-27632 to study the impact of ROCK inhibition on cell invasion in a low- and high-density environment. Treatment with Y-27632 resulted in a 3.5-fold increase in cell invasion for cells seeded at 50 × 103 cells/cm2.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3368365&req=5

fig4: SW620 cells were seeded at densities of 50 × 103 and 250 × 103 cells/cm2 onto 1.5 mg/mL collagen I gels. Scaffolds were treated with Y-27632 to study the impact of ROCK inhibition on cell invasion in a low- and high-density environment. Treatment with Y-27632 resulted in a 3.5-fold increase in cell invasion for cells seeded at 50 × 103 cells/cm2.
Mentions: Samples were tracked using a cell tracker dye. Seeding density did affect invasion depth of SW620 cells in collagen I scaffolds. Cells seeded at 250 × 103 cells/cm2 invaded twice the distance than those seeded at 50 × 103 cells/cm2 for untreated. However, this effect was not observed for SW620 cells treated with Y-27632. For cells seeded at the lower seeding density, treatment with Y-27632 led to a 3.5-fold increase in invasion depth. However, no significant increase was observed in cells seeded at 250 × 103 cells/cm2. Standard Student t-test was performed and a P value of 0.05 was considered significant (∗). The data is shown in Figure 4.

Bottom Line: Increasing collagen I scaffold density resulted in a 2.5-fold increase in cell proliferation while treatment with Y-27632 attenuated this effect although 1.5 fold increase in cell invasion was observed in ROCK inhibited samples.Intriguingly, ROCK inhibition also resulted in a 3.5-fold increase in cell invasion within 3D collagen scaffolds for cells seeded at lower densities.This data suggests that although ROCK inhibitors have been used clinically to treat several medical conditions, its effect largely depends on the surrounding microenvironment.

View Article: PubMed Central - PubMed

Affiliation: Department of Bioengineering, University of Illinois at Chicago, Chicago, IL 60612, USA.

ABSTRACT
The concept of using tissue density as a mechanism to diagnose a tumor has been around for centuries. However, this concept has not been sufficiently explored in a laboratory setting. Therefore, in this paper, we observed the effects of cell density and extracellular matrix (ECM) density on colon cancer invasion and proliferation using SW620 cells. We also attempted to inhibit ROCK-I to determine its effect on cell invasion and proliferation using standard molecular biology techniques and advanced imaging. Increasing cell seeding density resulted in a 2-fold increase in cell invasion as well as cell proliferation independent of treatment with Y-27632. Increasing collagen I scaffold density resulted in a 2.5-fold increase in cell proliferation while treatment with Y-27632 attenuated this effect although 1.5 fold increase in cell invasion was observed in ROCK inhibited samples. Intriguingly, ROCK inhibition also resulted in a 3.5-fold increase in cell invasion within 3D collagen scaffolds for cells seeded at lower densities. We show in this paper that ROCK-I inhibition leads to increased invasion within 3D collagen I microenvironments. This data suggests that although ROCK inhibitors have been used clinically to treat several medical conditions, its effect largely depends on the surrounding microenvironment.

No MeSH data available.


Related in: MedlinePlus