Limits...
Treatment with Y-27632, a ROCK Inhibitor, Increases the Proinvasive Nature of SW620 Cells on 3D Collagen Type 1 Matrix.

Vishnubhotla R, Bharadwaj S, Sun S, Metlushko V, Glover SC - Int J Cell Biol (2012)

Bottom Line: Increasing collagen I scaffold density resulted in a 2.5-fold increase in cell proliferation while treatment with Y-27632 attenuated this effect although 1.5 fold increase in cell invasion was observed in ROCK inhibited samples.Intriguingly, ROCK inhibition also resulted in a 3.5-fold increase in cell invasion within 3D collagen scaffolds for cells seeded at lower densities.This data suggests that although ROCK inhibitors have been used clinically to treat several medical conditions, its effect largely depends on the surrounding microenvironment.

View Article: PubMed Central - PubMed

Affiliation: Department of Bioengineering, University of Illinois at Chicago, Chicago, IL 60612, USA.

ABSTRACT
The concept of using tissue density as a mechanism to diagnose a tumor has been around for centuries. However, this concept has not been sufficiently explored in a laboratory setting. Therefore, in this paper, we observed the effects of cell density and extracellular matrix (ECM) density on colon cancer invasion and proliferation using SW620 cells. We also attempted to inhibit ROCK-I to determine its effect on cell invasion and proliferation using standard molecular biology techniques and advanced imaging. Increasing cell seeding density resulted in a 2-fold increase in cell invasion as well as cell proliferation independent of treatment with Y-27632. Increasing collagen I scaffold density resulted in a 2.5-fold increase in cell proliferation while treatment with Y-27632 attenuated this effect although 1.5 fold increase in cell invasion was observed in ROCK inhibited samples. Intriguingly, ROCK inhibition also resulted in a 3.5-fold increase in cell invasion within 3D collagen scaffolds for cells seeded at lower densities. We show in this paper that ROCK-I inhibition leads to increased invasion within 3D collagen I microenvironments. This data suggests that although ROCK inhibitors have been used clinically to treat several medical conditions, its effect largely depends on the surrounding microenvironment.

No MeSH data available.


Related in: MedlinePlus

ROCK activities of SW620 cells seeded at 50 × 103 and 250 × 103 cells/cm2 and allowed to invade into 1.5 mg/mL collagen scaffolds. Cells seeded at the higher density had over double the ROCK activity compared to those seeded at the lower density. The number of cells was kept constant between the two conditions while the seeding area was altered.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3368365&req=5

fig2: ROCK activities of SW620 cells seeded at 50 × 103 and 250 × 103 cells/cm2 and allowed to invade into 1.5 mg/mL collagen scaffolds. Cells seeded at the higher density had over double the ROCK activity compared to those seeded at the lower density. The number of cells was kept constant between the two conditions while the seeding area was altered.

Mentions: ROCK activity was significantly increased with an increase in cell density. A 2.5-fold increase in ROCK activity was observed in scaffolds with cells seeded at 250 × 103 cells/cm2 compared to scaffold with cells seeded at 50 × 103 cells/cm2. It is important to note that cell density was altered by changing the seeding area and keeping the number of cells seeded constant. This was done to determine the effect that cell density alone, and not the number of cells, has on ROCK activity. Standard Student t-test was performed and a P value of 0.05 was considered significant (∗). The data is shown in Figure 2.


Treatment with Y-27632, a ROCK Inhibitor, Increases the Proinvasive Nature of SW620 Cells on 3D Collagen Type 1 Matrix.

Vishnubhotla R, Bharadwaj S, Sun S, Metlushko V, Glover SC - Int J Cell Biol (2012)

ROCK activities of SW620 cells seeded at 50 × 103 and 250 × 103 cells/cm2 and allowed to invade into 1.5 mg/mL collagen scaffolds. Cells seeded at the higher density had over double the ROCK activity compared to those seeded at the lower density. The number of cells was kept constant between the two conditions while the seeding area was altered.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3368365&req=5

fig2: ROCK activities of SW620 cells seeded at 50 × 103 and 250 × 103 cells/cm2 and allowed to invade into 1.5 mg/mL collagen scaffolds. Cells seeded at the higher density had over double the ROCK activity compared to those seeded at the lower density. The number of cells was kept constant between the two conditions while the seeding area was altered.
Mentions: ROCK activity was significantly increased with an increase in cell density. A 2.5-fold increase in ROCK activity was observed in scaffolds with cells seeded at 250 × 103 cells/cm2 compared to scaffold with cells seeded at 50 × 103 cells/cm2. It is important to note that cell density was altered by changing the seeding area and keeping the number of cells seeded constant. This was done to determine the effect that cell density alone, and not the number of cells, has on ROCK activity. Standard Student t-test was performed and a P value of 0.05 was considered significant (∗). The data is shown in Figure 2.

Bottom Line: Increasing collagen I scaffold density resulted in a 2.5-fold increase in cell proliferation while treatment with Y-27632 attenuated this effect although 1.5 fold increase in cell invasion was observed in ROCK inhibited samples.Intriguingly, ROCK inhibition also resulted in a 3.5-fold increase in cell invasion within 3D collagen scaffolds for cells seeded at lower densities.This data suggests that although ROCK inhibitors have been used clinically to treat several medical conditions, its effect largely depends on the surrounding microenvironment.

View Article: PubMed Central - PubMed

Affiliation: Department of Bioengineering, University of Illinois at Chicago, Chicago, IL 60612, USA.

ABSTRACT
The concept of using tissue density as a mechanism to diagnose a tumor has been around for centuries. However, this concept has not been sufficiently explored in a laboratory setting. Therefore, in this paper, we observed the effects of cell density and extracellular matrix (ECM) density on colon cancer invasion and proliferation using SW620 cells. We also attempted to inhibit ROCK-I to determine its effect on cell invasion and proliferation using standard molecular biology techniques and advanced imaging. Increasing cell seeding density resulted in a 2-fold increase in cell invasion as well as cell proliferation independent of treatment with Y-27632. Increasing collagen I scaffold density resulted in a 2.5-fold increase in cell proliferation while treatment with Y-27632 attenuated this effect although 1.5 fold increase in cell invasion was observed in ROCK inhibited samples. Intriguingly, ROCK inhibition also resulted in a 3.5-fold increase in cell invasion within 3D collagen scaffolds for cells seeded at lower densities. We show in this paper that ROCK-I inhibition leads to increased invasion within 3D collagen I microenvironments. This data suggests that although ROCK inhibitors have been used clinically to treat several medical conditions, its effect largely depends on the surrounding microenvironment.

No MeSH data available.


Related in: MedlinePlus