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Metabonomic Evaluation of ZHENG Differentiation and Treatment by Fuzhenghuayu Tablet in Hepatitis-B-Caused Cirrhosis.

Sun S, Dai J, Wang W, Cao H, Fang J, Hu YY, Su S, Zhang Y - Evid Based Complement Alternat Med (2012)

Bottom Line: Urine samples of 12 healthy volunteers (control group, CG) and 31 HBC patients (HBCG) were analyzed by gas chromatography mass spectrometry (GC/MS) and multivariate statistical analysis.The significantly changed metabolites between CG and HBCG were selected by PLS-DA loading plot analysis.The efficiency of FZHY tablet on subjects with spleen deficiency with dampness encumbrance syndrome (SDDES) and liver-kidney yin deficiency syndrome (LKYDS) was better than that of other syndromes.

View Article: PubMed Central - PubMed

Affiliation: Research Center for Traditional Chinese Medicine and Systems Biology, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Pudong, Shanghai 201203, China.

ABSTRACT
In Traditional Chinese Medicine (TCM), treatment based on ZHENG (also called TCM syndrome and pattern) differentiation has been applied for about 3 thousand years, while there are some difficulties to communicate with western medicine. In the present work, metabonomic methods were utilized to differentiate ZHENG types and evaluate the therapeutic efficiency of Fuzhenghuayu (FZHY) tablet in hepatitis-B-caused cirrhosis (HBC). Urine samples of 12 healthy volunteers (control group, CG) and 31 HBC patients (HBCG) were analyzed by gas chromatography mass spectrometry (GC/MS) and multivariate statistical analysis. The significantly changed metabolites between CG and HBCG were selected by PLS-DA loading plot analysis. Moreover, 4 ZHENGs were differentiated mutually, suggesting that there was urine metabolic material basis in ZHENG differentiation. The efficiency of FZHY tablet on subjects with spleen deficiency with dampness encumbrance syndrome (SDDES) and liver-kidney yin deficiency syndrome (LKYDS) was better than that of other syndromes. The efficiency of FZHY treatment based on ZHENG differentiation indicated that accurately ZHENG differentiating could guide the appropriate TCM treatment in HBC.

No MeSH data available.


Related in: MedlinePlus

Five maps revealed the therapeutic effect of FZHY tablet for hepatitis-B-caused cirrhosis (HBC) and four TCM ZHENG types, respectively: spleen deficiency with dampness encumbrance syndrome (SDDES), liver-gallbladder dampness-heat syndrome (LGDHS), liver-kidney yin deficiency syndrome (LKYDS), and blood stasis syndrome (BSS), by the changing trend of significantly differential metabolites. CG is short for control group; preoral in each group stands for information before intervention of FZHY tablet; 12th week means effects after 12-week intervention by FZHY tablet; 24th week means effects after 24-week intervention. The x-axis represented the changed metabolites, and the y-axis was average rank in Mann-Whitney U-test, representing the contents of metabolites.
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Related In: Results  -  Collection


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fig4: Five maps revealed the therapeutic effect of FZHY tablet for hepatitis-B-caused cirrhosis (HBC) and four TCM ZHENG types, respectively: spleen deficiency with dampness encumbrance syndrome (SDDES), liver-gallbladder dampness-heat syndrome (LGDHS), liver-kidney yin deficiency syndrome (LKYDS), and blood stasis syndrome (BSS), by the changing trend of significantly differential metabolites. CG is short for control group; preoral in each group stands for information before intervention of FZHY tablet; 12th week means effects after 12-week intervention by FZHY tablet; 24th week means effects after 24-week intervention. The x-axis represented the changed metabolites, and the y-axis was average rank in Mann-Whitney U-test, representing the contents of metabolites.

Mentions: The significantly changed metabolites of each group HBC patients from the healthy subjects have been selected. And the potential biomarkers of HBCG were previously listed in Table 1. The four TCM ZHEGNs' potential biomarkers are not summarized in tables but can be found on x-axis of Figure 4. The reversions of these metabolites were based on to evaluate the therapeutic effect of FZHY tablet. Consequently, we found that there were no significantly reversed potential biomarkers for all the subjects of HBC at both 12th week and 24th week. While for LSYDS at 12th week, most influential metabolites reversed, and at 24th week, the reversion of potential biomarkers showed good efficiency of FZHY for SDDES, as manifested in Figure 4 (LKYDS and SDDES). As we can see, most metabolites for LGDHS and BBS got further away from the healthy group than pre-oral of FZHY.


Metabonomic Evaluation of ZHENG Differentiation and Treatment by Fuzhenghuayu Tablet in Hepatitis-B-Caused Cirrhosis.

Sun S, Dai J, Wang W, Cao H, Fang J, Hu YY, Su S, Zhang Y - Evid Based Complement Alternat Med (2012)

Five maps revealed the therapeutic effect of FZHY tablet for hepatitis-B-caused cirrhosis (HBC) and four TCM ZHENG types, respectively: spleen deficiency with dampness encumbrance syndrome (SDDES), liver-gallbladder dampness-heat syndrome (LGDHS), liver-kidney yin deficiency syndrome (LKYDS), and blood stasis syndrome (BSS), by the changing trend of significantly differential metabolites. CG is short for control group; preoral in each group stands for information before intervention of FZHY tablet; 12th week means effects after 12-week intervention by FZHY tablet; 24th week means effects after 24-week intervention. The x-axis represented the changed metabolites, and the y-axis was average rank in Mann-Whitney U-test, representing the contents of metabolites.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3368351&req=5

fig4: Five maps revealed the therapeutic effect of FZHY tablet for hepatitis-B-caused cirrhosis (HBC) and four TCM ZHENG types, respectively: spleen deficiency with dampness encumbrance syndrome (SDDES), liver-gallbladder dampness-heat syndrome (LGDHS), liver-kidney yin deficiency syndrome (LKYDS), and blood stasis syndrome (BSS), by the changing trend of significantly differential metabolites. CG is short for control group; preoral in each group stands for information before intervention of FZHY tablet; 12th week means effects after 12-week intervention by FZHY tablet; 24th week means effects after 24-week intervention. The x-axis represented the changed metabolites, and the y-axis was average rank in Mann-Whitney U-test, representing the contents of metabolites.
Mentions: The significantly changed metabolites of each group HBC patients from the healthy subjects have been selected. And the potential biomarkers of HBCG were previously listed in Table 1. The four TCM ZHEGNs' potential biomarkers are not summarized in tables but can be found on x-axis of Figure 4. The reversions of these metabolites were based on to evaluate the therapeutic effect of FZHY tablet. Consequently, we found that there were no significantly reversed potential biomarkers for all the subjects of HBC at both 12th week and 24th week. While for LSYDS at 12th week, most influential metabolites reversed, and at 24th week, the reversion of potential biomarkers showed good efficiency of FZHY for SDDES, as manifested in Figure 4 (LKYDS and SDDES). As we can see, most metabolites for LGDHS and BBS got further away from the healthy group than pre-oral of FZHY.

Bottom Line: Urine samples of 12 healthy volunteers (control group, CG) and 31 HBC patients (HBCG) were analyzed by gas chromatography mass spectrometry (GC/MS) and multivariate statistical analysis.The significantly changed metabolites between CG and HBCG were selected by PLS-DA loading plot analysis.The efficiency of FZHY tablet on subjects with spleen deficiency with dampness encumbrance syndrome (SDDES) and liver-kidney yin deficiency syndrome (LKYDS) was better than that of other syndromes.

View Article: PubMed Central - PubMed

Affiliation: Research Center for Traditional Chinese Medicine and Systems Biology, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Pudong, Shanghai 201203, China.

ABSTRACT
In Traditional Chinese Medicine (TCM), treatment based on ZHENG (also called TCM syndrome and pattern) differentiation has been applied for about 3 thousand years, while there are some difficulties to communicate with western medicine. In the present work, metabonomic methods were utilized to differentiate ZHENG types and evaluate the therapeutic efficiency of Fuzhenghuayu (FZHY) tablet in hepatitis-B-caused cirrhosis (HBC). Urine samples of 12 healthy volunteers (control group, CG) and 31 HBC patients (HBCG) were analyzed by gas chromatography mass spectrometry (GC/MS) and multivariate statistical analysis. The significantly changed metabolites between CG and HBCG were selected by PLS-DA loading plot analysis. Moreover, 4 ZHENGs were differentiated mutually, suggesting that there was urine metabolic material basis in ZHENG differentiation. The efficiency of FZHY tablet on subjects with spleen deficiency with dampness encumbrance syndrome (SDDES) and liver-kidney yin deficiency syndrome (LKYDS) was better than that of other syndromes. The efficiency of FZHY treatment based on ZHENG differentiation indicated that accurately ZHENG differentiating could guide the appropriate TCM treatment in HBC.

No MeSH data available.


Related in: MedlinePlus