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Metabonomic Evaluation of ZHENG Differentiation and Treatment by Fuzhenghuayu Tablet in Hepatitis-B-Caused Cirrhosis.

Sun S, Dai J, Wang W, Cao H, Fang J, Hu YY, Su S, Zhang Y - Evid Based Complement Alternat Med (2012)

Bottom Line: Urine samples of 12 healthy volunteers (control group, CG) and 31 HBC patients (HBCG) were analyzed by gas chromatography mass spectrometry (GC/MS) and multivariate statistical analysis.The significantly changed metabolites between CG and HBCG were selected by PLS-DA loading plot analysis.The efficiency of FZHY tablet on subjects with spleen deficiency with dampness encumbrance syndrome (SDDES) and liver-kidney yin deficiency syndrome (LKYDS) was better than that of other syndromes.

View Article: PubMed Central - PubMed

Affiliation: Research Center for Traditional Chinese Medicine and Systems Biology, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Pudong, Shanghai 201203, China.

ABSTRACT
In Traditional Chinese Medicine (TCM), treatment based on ZHENG (also called TCM syndrome and pattern) differentiation has been applied for about 3 thousand years, while there are some difficulties to communicate with western medicine. In the present work, metabonomic methods were utilized to differentiate ZHENG types and evaluate the therapeutic efficiency of Fuzhenghuayu (FZHY) tablet in hepatitis-B-caused cirrhosis (HBC). Urine samples of 12 healthy volunteers (control group, CG) and 31 HBC patients (HBCG) were analyzed by gas chromatography mass spectrometry (GC/MS) and multivariate statistical analysis. The significantly changed metabolites between CG and HBCG were selected by PLS-DA loading plot analysis. Moreover, 4 ZHENGs were differentiated mutually, suggesting that there was urine metabolic material basis in ZHENG differentiation. The efficiency of FZHY tablet on subjects with spleen deficiency with dampness encumbrance syndrome (SDDES) and liver-kidney yin deficiency syndrome (LKYDS) was better than that of other syndromes. The efficiency of FZHY treatment based on ZHENG differentiation indicated that accurately ZHENG differentiating could guide the appropriate TCM treatment in HBC.

No MeSH data available.


Related in: MedlinePlus

(a) PLS-DA score plot between CG and HBCG. Black dots and red triangles refer to healthy subjects and hepatitis-B-caused Cirrhosis subjects, respectively. (b) PLS-DA loading plot from HBCG and CG.
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fig1: (a) PLS-DA score plot between CG and HBCG. Black dots and red triangles refer to healthy subjects and hepatitis-B-caused Cirrhosis subjects, respectively. (b) PLS-DA loading plot from HBCG and CG.

Mentions: One μL aliquots of supernatants of all the urine samples, after a two-step derivatization, extraction and dryness, were injected into GC/MS for analysis with the method described previously. PLS-DA analysis was employed to discriminate HBCG and CG, and the score plot with R2Y = 0.888 and Q2Y = 0.792 is shown in Figure 1(a). In this map, HBCG could be absolutely separated from healthy group. The results might demonstrate that the urine metabolic profiles had changed significantly.


Metabonomic Evaluation of ZHENG Differentiation and Treatment by Fuzhenghuayu Tablet in Hepatitis-B-Caused Cirrhosis.

Sun S, Dai J, Wang W, Cao H, Fang J, Hu YY, Su S, Zhang Y - Evid Based Complement Alternat Med (2012)

(a) PLS-DA score plot between CG and HBCG. Black dots and red triangles refer to healthy subjects and hepatitis-B-caused Cirrhosis subjects, respectively. (b) PLS-DA loading plot from HBCG and CG.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3368351&req=5

fig1: (a) PLS-DA score plot between CG and HBCG. Black dots and red triangles refer to healthy subjects and hepatitis-B-caused Cirrhosis subjects, respectively. (b) PLS-DA loading plot from HBCG and CG.
Mentions: One μL aliquots of supernatants of all the urine samples, after a two-step derivatization, extraction and dryness, were injected into GC/MS for analysis with the method described previously. PLS-DA analysis was employed to discriminate HBCG and CG, and the score plot with R2Y = 0.888 and Q2Y = 0.792 is shown in Figure 1(a). In this map, HBCG could be absolutely separated from healthy group. The results might demonstrate that the urine metabolic profiles had changed significantly.

Bottom Line: Urine samples of 12 healthy volunteers (control group, CG) and 31 HBC patients (HBCG) were analyzed by gas chromatography mass spectrometry (GC/MS) and multivariate statistical analysis.The significantly changed metabolites between CG and HBCG were selected by PLS-DA loading plot analysis.The efficiency of FZHY tablet on subjects with spleen deficiency with dampness encumbrance syndrome (SDDES) and liver-kidney yin deficiency syndrome (LKYDS) was better than that of other syndromes.

View Article: PubMed Central - PubMed

Affiliation: Research Center for Traditional Chinese Medicine and Systems Biology, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Pudong, Shanghai 201203, China.

ABSTRACT
In Traditional Chinese Medicine (TCM), treatment based on ZHENG (also called TCM syndrome and pattern) differentiation has been applied for about 3 thousand years, while there are some difficulties to communicate with western medicine. In the present work, metabonomic methods were utilized to differentiate ZHENG types and evaluate the therapeutic efficiency of Fuzhenghuayu (FZHY) tablet in hepatitis-B-caused cirrhosis (HBC). Urine samples of 12 healthy volunteers (control group, CG) and 31 HBC patients (HBCG) were analyzed by gas chromatography mass spectrometry (GC/MS) and multivariate statistical analysis. The significantly changed metabolites between CG and HBCG were selected by PLS-DA loading plot analysis. Moreover, 4 ZHENGs were differentiated mutually, suggesting that there was urine metabolic material basis in ZHENG differentiation. The efficiency of FZHY tablet on subjects with spleen deficiency with dampness encumbrance syndrome (SDDES) and liver-kidney yin deficiency syndrome (LKYDS) was better than that of other syndromes. The efficiency of FZHY treatment based on ZHENG differentiation indicated that accurately ZHENG differentiating could guide the appropriate TCM treatment in HBC.

No MeSH data available.


Related in: MedlinePlus