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Evaluation of Hepatoprotective Effect of Leaves of Cassia sophera Linn.

Mondal A, Karan SK, Singha T, Rajalingam D, Maity TK - Evid Based Complement Alternat Med (2012)

Bottom Line: The substantially elevated serum enzymatic levels of AST, ALT, ALP, and total bilirubin were restored towards the normalization significantly by the extracts.The decreased serum total protein level was significantly normalized.Silymarin was used as standard reference and exhibited significant hepatoprotective activity against carbon tetrachloride-induced hepatotoxicity in rats.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Technology, Jadavpur University, Jadavpur, West Bengal, Kolkata 700 032, India.

ABSTRACT
In the present study, the hepatoprotective activity of ethanolic extracts of Cassia sophera Linn. leaves was evaluated against carbon-tetrachloride- (CCl(4)-) induced hepatic damage in rats. The extracts at doses of 200 and 400 mg/kg were administered orally once daily. The hepatoprotection was assessed in terms of reduction in histological damage, changes in serum enzymes, serum glutamate oxaloacetate transaminase (AST), serum glutamate pyruvate transaminase (ALT), serum alkaline phosphatase (ALP), total bilirubin, and total protein levels. The substantially elevated serum enzymatic levels of AST, ALT, ALP, and total bilirubin were restored towards the normalization significantly by the extracts. The decreased serum total protein level was significantly normalized. Silymarin was used as standard reference and exhibited significant hepatoprotective activity against carbon tetrachloride-induced hepatotoxicity in rats. The biochemical observations were supplemented with histopathological examination of rat liver sections. The results of this study strongly indicate that Cassia sophera leaves have potent hepatoprotective action against carbon tetrachloride-induced hepatic damage in rats. This study suggests that possible activity may be due to the presence of flavonoids in the extracts.

No MeSH data available.


Related in: MedlinePlus

Photomicroscopy of liver sections from CCl4-intoxicated rats (10x). Histological observations of the liver tissue of the normal animals showed hepatic cells with well-preserved cytoplasm, nucleus, nucleolus, and central vein (a). Treatment with CCl4 caused fatty degeneration with severe necrosis of the parenchyma cells in the central lobular region of the liver. Furthermore, hepatocytic necrosis was predominant surrounding the central vein, which formed a streak-like appearance (b), (c), (d), and (e) showed animals treated with EECS (200 and 400 mg/kg, p.o.) and silymarin (25 mg/kg) and restored the altered histopathological changes, respectively.
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fig2: Photomicroscopy of liver sections from CCl4-intoxicated rats (10x). Histological observations of the liver tissue of the normal animals showed hepatic cells with well-preserved cytoplasm, nucleus, nucleolus, and central vein (a). Treatment with CCl4 caused fatty degeneration with severe necrosis of the parenchyma cells in the central lobular region of the liver. Furthermore, hepatocytic necrosis was predominant surrounding the central vein, which formed a streak-like appearance (b), (c), (d), and (e) showed animals treated with EECS (200 and 400 mg/kg, p.o.) and silymarin (25 mg/kg) and restored the altered histopathological changes, respectively.

Mentions: Histological observations of the liver tissue of the normal animals showed hepatic cells with well-preserved cytoplasm, nucleus, nucleolus, and central vein (Figure 2(a); 10x). Treatment with CCl4 caused fatty degeneration with severe necrosis of the parenchyma cells in the central lobular region of the liver. Furthermore, hepatocytic necrosis was predominant surrounding the central vein, which formed a streak-like appearance (Figure 2(b); 10x). Figures 2(c), 2(d) and 2(e) (10x) show animals treated with EECS (200 and 400 mg/kg, p.o.) and Silymarin (25 mg/kg) and restored the altered histopathological changes, respectively.


Evaluation of Hepatoprotective Effect of Leaves of Cassia sophera Linn.

Mondal A, Karan SK, Singha T, Rajalingam D, Maity TK - Evid Based Complement Alternat Med (2012)

Photomicroscopy of liver sections from CCl4-intoxicated rats (10x). Histological observations of the liver tissue of the normal animals showed hepatic cells with well-preserved cytoplasm, nucleus, nucleolus, and central vein (a). Treatment with CCl4 caused fatty degeneration with severe necrosis of the parenchyma cells in the central lobular region of the liver. Furthermore, hepatocytic necrosis was predominant surrounding the central vein, which formed a streak-like appearance (b), (c), (d), and (e) showed animals treated with EECS (200 and 400 mg/kg, p.o.) and silymarin (25 mg/kg) and restored the altered histopathological changes, respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3368335&req=5

fig2: Photomicroscopy of liver sections from CCl4-intoxicated rats (10x). Histological observations of the liver tissue of the normal animals showed hepatic cells with well-preserved cytoplasm, nucleus, nucleolus, and central vein (a). Treatment with CCl4 caused fatty degeneration with severe necrosis of the parenchyma cells in the central lobular region of the liver. Furthermore, hepatocytic necrosis was predominant surrounding the central vein, which formed a streak-like appearance (b), (c), (d), and (e) showed animals treated with EECS (200 and 400 mg/kg, p.o.) and silymarin (25 mg/kg) and restored the altered histopathological changes, respectively.
Mentions: Histological observations of the liver tissue of the normal animals showed hepatic cells with well-preserved cytoplasm, nucleus, nucleolus, and central vein (Figure 2(a); 10x). Treatment with CCl4 caused fatty degeneration with severe necrosis of the parenchyma cells in the central lobular region of the liver. Furthermore, hepatocytic necrosis was predominant surrounding the central vein, which formed a streak-like appearance (Figure 2(b); 10x). Figures 2(c), 2(d) and 2(e) (10x) show animals treated with EECS (200 and 400 mg/kg, p.o.) and Silymarin (25 mg/kg) and restored the altered histopathological changes, respectively.

Bottom Line: The substantially elevated serum enzymatic levels of AST, ALT, ALP, and total bilirubin were restored towards the normalization significantly by the extracts.The decreased serum total protein level was significantly normalized.Silymarin was used as standard reference and exhibited significant hepatoprotective activity against carbon tetrachloride-induced hepatotoxicity in rats.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Technology, Jadavpur University, Jadavpur, West Bengal, Kolkata 700 032, India.

ABSTRACT
In the present study, the hepatoprotective activity of ethanolic extracts of Cassia sophera Linn. leaves was evaluated against carbon-tetrachloride- (CCl(4)-) induced hepatic damage in rats. The extracts at doses of 200 and 400 mg/kg were administered orally once daily. The hepatoprotection was assessed in terms of reduction in histological damage, changes in serum enzymes, serum glutamate oxaloacetate transaminase (AST), serum glutamate pyruvate transaminase (ALT), serum alkaline phosphatase (ALP), total bilirubin, and total protein levels. The substantially elevated serum enzymatic levels of AST, ALT, ALP, and total bilirubin were restored towards the normalization significantly by the extracts. The decreased serum total protein level was significantly normalized. Silymarin was used as standard reference and exhibited significant hepatoprotective activity against carbon tetrachloride-induced hepatotoxicity in rats. The biochemical observations were supplemented with histopathological examination of rat liver sections. The results of this study strongly indicate that Cassia sophera leaves have potent hepatoprotective action against carbon tetrachloride-induced hepatic damage in rats. This study suggests that possible activity may be due to the presence of flavonoids in the extracts.

No MeSH data available.


Related in: MedlinePlus