Single-shot full-field reflection phase microscopy.
Bottom Line: The reflection-based DHM provides highly sensitive and a single-shot imaging of cellular dynamics while the use of low coherence source provides a depth-selective measurement.The setup uniquely uses a diffraction grating in the reference arm to generate an interference image of uniform contrast over the entire field-of-view albeit low-coherence light source.We have measured the path-length sensitivity of our instrument to be approximately 21 picometers/Hz that makes it suitable for nanometer-scale full-field measurement of membrane dynamics in live cells.
Affiliation: G. R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA. firstname.lastname@example.orgShow MeSH
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Mentions: To demonstrate our system’s capabilities, we have studied the membrane fluctuations in HeLa cells under different cell conditions. More specifically, we prepared (i) a sample of living normal HeLa cells, (ii) a fixed HeLa cell sample after treatment with 2% paraformaldehyde and (iii) a sample of HeLa cells treated with 8 nM Cytochalasin-D which inhibits actin polymerization . The frame rate of the image acquisition was set to 1 kHz and the data was recorded for duration of 1 sec for each cell. As shown in Fig. 5(a)Fig. 5
Affiliation: G. R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA. email@example.com