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Single-shot full-field reflection phase microscopy.

Yaqoob Z, Yamauchi T, Choi W, Fu D, Dasari RR, Feld MS - Opt Express (2011)

Bottom Line: The reflection-based DHM provides highly sensitive and a single-shot imaging of cellular dynamics while the use of low coherence source provides a depth-selective measurement.The setup uniquely uses a diffraction grating in the reference arm to generate an interference image of uniform contrast over the entire field-of-view albeit low-coherence light source.We have measured the path-length sensitivity of our instrument to be approximately 21 picometers/Hz that makes it suitable for nanometer-scale full-field measurement of membrane dynamics in live cells.

View Article: PubMed Central - PubMed

Affiliation: G. R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA. zyaqoob@mit.edu

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(a,b) Location of coherence gate for double-pass transmission and reflection phase imaging, respectively. (c) Double-pass transmission phase image of a HeLa cell, and (d) Single-shot reflection phase image of the region inside square box in (c).
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g004: (a,b) Location of coherence gate for double-pass transmission and reflection phase imaging, respectively. (c) Double-pass transmission phase image of a HeLa cell, and (d) Single-shot reflection phase image of the region inside square box in (c).

Mentions: To demonstrate high-speed quantitative imaging of live cells, we sub-cultured HeLa cells on glass slides 2 days before the measurement and immersed in standard culture medium (Dulbecco's Modified Eagle Medium). As mentioned earlier, our setup is capable of taking transmission phase images as well as reflection phase images. Figures 4(a)Fig. 4


Single-shot full-field reflection phase microscopy.

Yaqoob Z, Yamauchi T, Choi W, Fu D, Dasari RR, Feld MS - Opt Express (2011)

(a,b) Location of coherence gate for double-pass transmission and reflection phase imaging, respectively. (c) Double-pass transmission phase image of a HeLa cell, and (d) Single-shot reflection phase image of the region inside square box in (c).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3368324&req=5

g004: (a,b) Location of coherence gate for double-pass transmission and reflection phase imaging, respectively. (c) Double-pass transmission phase image of a HeLa cell, and (d) Single-shot reflection phase image of the region inside square box in (c).
Mentions: To demonstrate high-speed quantitative imaging of live cells, we sub-cultured HeLa cells on glass slides 2 days before the measurement and immersed in standard culture medium (Dulbecco's Modified Eagle Medium). As mentioned earlier, our setup is capable of taking transmission phase images as well as reflection phase images. Figures 4(a)Fig. 4

Bottom Line: The reflection-based DHM provides highly sensitive and a single-shot imaging of cellular dynamics while the use of low coherence source provides a depth-selective measurement.The setup uniquely uses a diffraction grating in the reference arm to generate an interference image of uniform contrast over the entire field-of-view albeit low-coherence light source.We have measured the path-length sensitivity of our instrument to be approximately 21 picometers/Hz that makes it suitable for nanometer-scale full-field measurement of membrane dynamics in live cells.

View Article: PubMed Central - PubMed

Affiliation: G. R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA. zyaqoob@mit.edu

Show MeSH
Related in: MedlinePlus