Single-shot full-field reflection phase microscopy.
Bottom Line: The reflection-based DHM provides highly sensitive and a single-shot imaging of cellular dynamics while the use of low coherence source provides a depth-selective measurement.The setup uniquely uses a diffraction grating in the reference arm to generate an interference image of uniform contrast over the entire field-of-view albeit low-coherence light source.We have measured the path-length sensitivity of our instrument to be approximately 21 picometers/Hz that makes it suitable for nanometer-scale full-field measurement of membrane dynamics in live cells.
Affiliation: G. R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA. firstname.lastname@example.orgShow MeSH
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Mentions: To demonstrate high-speed quantitative imaging of live cells, we sub-cultured HeLa cells on glass slides 2 days before the measurement and immersed in standard culture medium (Dulbecco's Modified Eagle Medium). As mentioned earlier, our setup is capable of taking transmission phase images as well as reflection phase images. Figures 4(a)Fig. 4
Affiliation: G. R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA. email@example.com