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Single-shot full-field reflection phase microscopy.

Yaqoob Z, Yamauchi T, Choi W, Fu D, Dasari RR, Feld MS - Opt Express (2011)

Bottom Line: The reflection-based DHM provides highly sensitive and a single-shot imaging of cellular dynamics while the use of low coherence source provides a depth-selective measurement.The setup uniquely uses a diffraction grating in the reference arm to generate an interference image of uniform contrast over the entire field-of-view albeit low-coherence light source.We have measured the path-length sensitivity of our instrument to be approximately 21 picometers/Hz that makes it suitable for nanometer-scale full-field measurement of membrane dynamics in live cells.

View Article: PubMed Central - PubMed

Affiliation: G. R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA. zyaqoob@mit.edu

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(a) Configuration to determine the sensitivity of FF-RPM. and (b) Measured phase fluctuation (radian) as a function of applied voltage. Mi: ith mirror, PZT: Lead Zirconate Titanate.
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g003: (a) Configuration to determine the sensitivity of FF-RPM. and (b) Measured phase fluctuation (radian) as a function of applied voltage. Mi: ith mirror, PZT: Lead Zirconate Titanate.

Mentions: Intrinsic membrane fluctuations in living cells are typically on the order of a nanometer or less; the measurement of these small membrane fluctuations requires the development of quantitative phase microscopes with high signal-to-noise ratio (SNR). In this section, we show the measurement sensitivity of our full-field RPM in terms of the least detectable axial motion; the configuration to measure the measurement sensitivity is shown in Fig. 3(a)Fig. 3


Single-shot full-field reflection phase microscopy.

Yaqoob Z, Yamauchi T, Choi W, Fu D, Dasari RR, Feld MS - Opt Express (2011)

(a) Configuration to determine the sensitivity of FF-RPM. and (b) Measured phase fluctuation (radian) as a function of applied voltage. Mi: ith mirror, PZT: Lead Zirconate Titanate.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3368324&req=5

g003: (a) Configuration to determine the sensitivity of FF-RPM. and (b) Measured phase fluctuation (radian) as a function of applied voltage. Mi: ith mirror, PZT: Lead Zirconate Titanate.
Mentions: Intrinsic membrane fluctuations in living cells are typically on the order of a nanometer or less; the measurement of these small membrane fluctuations requires the development of quantitative phase microscopes with high signal-to-noise ratio (SNR). In this section, we show the measurement sensitivity of our full-field RPM in terms of the least detectable axial motion; the configuration to measure the measurement sensitivity is shown in Fig. 3(a)Fig. 3

Bottom Line: The reflection-based DHM provides highly sensitive and a single-shot imaging of cellular dynamics while the use of low coherence source provides a depth-selective measurement.The setup uniquely uses a diffraction grating in the reference arm to generate an interference image of uniform contrast over the entire field-of-view albeit low-coherence light source.We have measured the path-length sensitivity of our instrument to be approximately 21 picometers/Hz that makes it suitable for nanometer-scale full-field measurement of membrane dynamics in live cells.

View Article: PubMed Central - PubMed

Affiliation: G. R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA. zyaqoob@mit.edu

Show MeSH
Related in: MedlinePlus