Single-shot full-field reflection phase microscopy.
Bottom Line: The reflection-based DHM provides highly sensitive and a single-shot imaging of cellular dynamics while the use of low coherence source provides a depth-selective measurement.The setup uniquely uses a diffraction grating in the reference arm to generate an interference image of uniform contrast over the entire field-of-view albeit low-coherence light source.We have measured the path-length sensitivity of our instrument to be approximately 21 picometers/Hz that makes it suitable for nanometer-scale full-field measurement of membrane dynamics in live cells.
Affiliation: G. R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA. email@example.comShow MeSH
Related in: MedlinePlus
Mentions: Intrinsic membrane fluctuations in living cells are typically on the order of a nanometer or less; the measurement of these small membrane fluctuations requires the development of quantitative phase microscopes with high signal-to-noise ratio (SNR). In this section, we show the measurement sensitivity of our full-field RPM in terms of the least detectable axial motion; the configuration to measure the measurement sensitivity is shown in Fig. 3(a)Fig. 3
Affiliation: G. R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA. firstname.lastname@example.org