Limits...
Single-shot full-field reflection phase microscopy.

Yaqoob Z, Yamauchi T, Choi W, Fu D, Dasari RR, Feld MS - Opt Express (2011)

Bottom Line: The reflection-based DHM provides highly sensitive and a single-shot imaging of cellular dynamics while the use of low coherence source provides a depth-selective measurement.The setup uniquely uses a diffraction grating in the reference arm to generate an interference image of uniform contrast over the entire field-of-view albeit low-coherence light source.We have measured the path-length sensitivity of our instrument to be approximately 21 picometers/Hz that makes it suitable for nanometer-scale full-field measurement of membrane dynamics in live cells.

View Article: PubMed Central - PubMed

Affiliation: G. R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA. zyaqoob@mit.edu

Show MeSH
Surface profile of a 40 microns diameter polystyrene microsphere measured using our single-shot full-field reflection phase microscope. (a) Interferogram after subtracting the no fringe image representing the DC signal, (b) amplitude component of the 2-dimensional Fourier transform of (a), (c) spatially filtered image of (b), (d) phase component of the inverse Fourier transform of (c), and (e) unwrapped phase image derived from (d).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3368324&req=5

g002: Surface profile of a 40 microns diameter polystyrene microsphere measured using our single-shot full-field reflection phase microscope. (a) Interferogram after subtracting the no fringe image representing the DC signal, (b) amplitude component of the 2-dimensional Fourier transform of (a), (c) spatially filtered image of (b), (d) phase component of the inverse Fourier transform of (c), and (e) unwrapped phase image derived from (d).

Mentions: Figure 2 (a)Fig. 2


Single-shot full-field reflection phase microscopy.

Yaqoob Z, Yamauchi T, Choi W, Fu D, Dasari RR, Feld MS - Opt Express (2011)

Surface profile of a 40 microns diameter polystyrene microsphere measured using our single-shot full-field reflection phase microscope. (a) Interferogram after subtracting the no fringe image representing the DC signal, (b) amplitude component of the 2-dimensional Fourier transform of (a), (c) spatially filtered image of (b), (d) phase component of the inverse Fourier transform of (c), and (e) unwrapped phase image derived from (d).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3368324&req=5

g002: Surface profile of a 40 microns diameter polystyrene microsphere measured using our single-shot full-field reflection phase microscope. (a) Interferogram after subtracting the no fringe image representing the DC signal, (b) amplitude component of the 2-dimensional Fourier transform of (a), (c) spatially filtered image of (b), (d) phase component of the inverse Fourier transform of (c), and (e) unwrapped phase image derived from (d).
Mentions: Figure 2 (a)Fig. 2

Bottom Line: The reflection-based DHM provides highly sensitive and a single-shot imaging of cellular dynamics while the use of low coherence source provides a depth-selective measurement.The setup uniquely uses a diffraction grating in the reference arm to generate an interference image of uniform contrast over the entire field-of-view albeit low-coherence light source.We have measured the path-length sensitivity of our instrument to be approximately 21 picometers/Hz that makes it suitable for nanometer-scale full-field measurement of membrane dynamics in live cells.

View Article: PubMed Central - PubMed

Affiliation: G. R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA. zyaqoob@mit.edu

Show MeSH