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Single-shot full-field reflection phase microscopy.

Yaqoob Z, Yamauchi T, Choi W, Fu D, Dasari RR, Feld MS - Opt Express (2011)

Bottom Line: The reflection-based DHM provides highly sensitive and a single-shot imaging of cellular dynamics while the use of low coherence source provides a depth-selective measurement.The setup uniquely uses a diffraction grating in the reference arm to generate an interference image of uniform contrast over the entire field-of-view albeit low-coherence light source.We have measured the path-length sensitivity of our instrument to be approximately 21 picometers/Hz that makes it suitable for nanometer-scale full-field measurement of membrane dynamics in live cells.

View Article: PubMed Central - PubMed

Affiliation: G. R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA. zyaqoob@mit.edu

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(a) Schematic of full-field single-shot reflection phase microscope. SMF: single mode fiber, Li: ith spherical lens, BSi: ith beam splitter, G: diffraction grating, Si: ith spatial filter. (b) Typical interferogram with a flat surface as the sample after subtracting the no fringe image representing the DC signal.
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g001: (a) Schematic of full-field single-shot reflection phase microscope. SMF: single mode fiber, Li: ith spherical lens, BSi: ith beam splitter, G: diffraction grating, Si: ith spatial filter. (b) Typical interferogram with a flat surface as the sample after subtracting the no fringe image representing the DC signal.

Mentions: Figure 1Fig. 1


Single-shot full-field reflection phase microscopy.

Yaqoob Z, Yamauchi T, Choi W, Fu D, Dasari RR, Feld MS - Opt Express (2011)

(a) Schematic of full-field single-shot reflection phase microscope. SMF: single mode fiber, Li: ith spherical lens, BSi: ith beam splitter, G: diffraction grating, Si: ith spatial filter. (b) Typical interferogram with a flat surface as the sample after subtracting the no fringe image representing the DC signal.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3368324&req=5

g001: (a) Schematic of full-field single-shot reflection phase microscope. SMF: single mode fiber, Li: ith spherical lens, BSi: ith beam splitter, G: diffraction grating, Si: ith spatial filter. (b) Typical interferogram with a flat surface as the sample after subtracting the no fringe image representing the DC signal.
Mentions: Figure 1Fig. 1

Bottom Line: The reflection-based DHM provides highly sensitive and a single-shot imaging of cellular dynamics while the use of low coherence source provides a depth-selective measurement.The setup uniquely uses a diffraction grating in the reference arm to generate an interference image of uniform contrast over the entire field-of-view albeit low-coherence light source.We have measured the path-length sensitivity of our instrument to be approximately 21 picometers/Hz that makes it suitable for nanometer-scale full-field measurement of membrane dynamics in live cells.

View Article: PubMed Central - PubMed

Affiliation: G. R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA. zyaqoob@mit.edu

Show MeSH