Comparison of STWRFS results with pathology analysis an

Dynamic tissue analysis using time- and wavelength-resolved fluorescence spectroscopy for atherosclerosis diagnosis. Sun Y, Sun Y, Stephens D, Xie H, Phipps J, Saroufeem R, Southard J, Elson DS, Marcu L - Opt Express (2011) Bottom Line: Distinct compositions of excised human atherosclerotic aorta were clearly discriminated over scanning lengths of several centimeters based on fluorescence lifetime and the intensity ratio between 390 and 452 nm.Operation of STWRFS blood flow was further validated in pig femoral arteries in vivo using a single-fiber probe integrated with an ultrasound imaging catheter.Current results demonstrate the potential of STWRFS as a tool for real-time optical characterization of arterial tissue composition and for atherosclerosis research and diagnosis. View Article: PubMed Central - PubMed Affiliation: Department of Biomedical Engineering, University of California Davis, Davis, CA 95616, USA. ABSTRACT Simultaneous time- and wavelength-resolved fluorescence spectroscopy (STWRFS) was developed and tested for the dynamic characterization of atherosclerotic tissue ex vivo and arterial vessels in vivo. Autofluorescence, induced by a 337 nm, 700 ps pulsed laser, was split to three wavelength sub-bands using dichroic filters, with each sub-band coupled into a different length of optical fiber for temporal separation. STWRFS allows for fast recording/analysis (few microseconds) of time-resolved fluorescence emission in these sub-bands and rapid scanning. Distinct compositions of excised human atherosclerotic aorta were clearly discriminated over scanning lengths of several centimeters based on fluorescence lifetime and the intensity ratio between 390 and 452 nm. Operation of STWRFS blood flow was further validated in pig femoral arteries in vivo using a single-fiber probe integrated with an ultrasound imaging catheter. Current results demonstrate the potential of STWRFS as a tool for real-time optical characterization of arterial tissue composition and for atherosclerosis research and diagnosis. Show MeSH Major Atherosclerosis/diagnosis* Signal Processing, Computer-Assisted/instrumentation* Spectrometry, Fluorescence/instrumentation* Minor Animals Equipment Design Equipment Failure Analysis Reproducibility of Results Sensitivity and Specificity Swine Related in: MedlinePlus	© Copyright Policy - open-access Related In: Results - Collection License Show All Figures getmorefigures.php?uid=PMC3368314&req=5 g004: Comparison of STWRFS results with pathology analysis and ultrasound imaging: (a) Close-up photograph of the tissue scanned through three regions: surrounding normal, early-stage plaque, and fibrotic plaque. The arrow indicates the scanning direction. (b) Fluorescence lifetime and intensity ratio show differentiation of three regions (scan time ~10 sec). (c) Ultrasound image of the cross section of the artery showing structural feature of the plaque. (d) Histopathology results (thricrome staining) used to confirmed the classification of normal (A-area), early-stage lipidic plaque (B-area), and fibrotic plaque (C-area) consistent with our interpretation of the STWRFS measurement. IT – intima layer; MD – media layer; LP – lipid rich area within thickened intima; FP – collagen rich area within intima; NC – necrotic area within intima. Mentions: A second scanning measurement of a different human aorta sample is summarized in Fig. 4Fig. 4

Dynamic tissue analysis using time- and wavelength-resolved fluorescence spectroscopy for atherosclerosis diagnosis.

Sun Y, Sun Y, Stephens D, Xie H, Phipps J, Saroufeem R, Southard J, Elson DS, Marcu L - Opt Express (2011)

Bottom Line: Distinct compositions of excised human atherosclerotic aorta were clearly discriminated over scanning lengths of several centimeters based on fluorescence lifetime and the intensity ratio between 390 and 452 nm.Operation of STWRFS blood flow was further validated in pig femoral arteries in vivo using a single-fiber probe integrated with an ultrasound imaging catheter.Current results demonstrate the potential of STWRFS as a tool for real-time optical characterization of arterial tissue composition and for atherosclerosis research and diagnosis.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Engineering, University of California Davis, Davis, CA 95616, USA.

ABSTRACT

Simultaneous time- and wavelength-resolved fluorescence spectroscopy (STWRFS) was developed and tested for the dynamic characterization of atherosclerotic tissue ex vivo and arterial vessels in vivo. Autofluorescence, induced by a 337 nm, 700 ps pulsed laser, was split to three wavelength sub-bands using dichroic filters, with each sub-band coupled into a different length of optical fiber for temporal separation. STWRFS allows for fast recording/analysis (few microseconds) of time-resolved fluorescence emission in these sub-bands and rapid scanning. Distinct compositions of excised human atherosclerotic aorta were clearly discriminated over scanning lengths of several centimeters based on fluorescence lifetime and the intensity ratio between 390 and 452 nm. Operation of STWRFS blood flow was further validated in pig femoral arteries in vivo using a single-fiber probe integrated with an ultrasound imaging catheter. Current results demonstrate the potential of STWRFS as a tool for real-time optical characterization of arterial tissue composition and for atherosclerosis research and diagnosis.

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Related in: MedlinePlus

Comparison of STWRFS results with pathology analysis and ultrasound imaging: (a) Close-up photograph of the tissue scanned through three regions: surrounding normal, early-stage plaque, and fibrotic plaque. The arrow indicates the scanning direction. (b) Fluorescence lifetime and intensity ratio show differentiation of three regions (scan time ~10 sec). (c) Ultrasound image of the cross section of the artery showing structural feature of the plaque. (d) Histopathology results (thricrome staining) used to confirmed the classification of normal (A-area), early-stage lipidic plaque (B-area), and fibrotic plaque (C-area) consistent with our interpretation of the STWRFS measurement. IT – intima layer; MD – media layer; LP – lipid rich area within thickened intima; FP – collagen rich area within intima; NC – necrotic area within intima.

Related In: Results - Collection

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g004: Comparison of STWRFS results with pathology analysis and ultrasound imaging: (a) Close-up photograph of the tissue scanned through three regions: surrounding normal, early-stage plaque, and fibrotic plaque. The arrow indicates the scanning direction. (b) Fluorescence lifetime and intensity ratio show differentiation of three regions (scan time ~10 sec). (c) Ultrasound image of the cross section of the artery showing structural feature of the plaque. (d) Histopathology results (thricrome staining) used to confirmed the classification of normal (A-area), early-stage lipidic plaque (B-area), and fibrotic plaque (C-area) consistent with our interpretation of the STWRFS measurement. IT – intima layer; MD – media layer; LP – lipid rich area within thickened intima; FP – collagen rich area within intima; NC – necrotic area within intima.

Mentions: A second scanning measurement of a different human aorta sample is summarized in Fig. 4Fig. 4