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Dynamic tissue analysis using time- and wavelength-resolved fluorescence spectroscopy for atherosclerosis diagnosis.

Sun Y, Sun Y, Stephens D, Xie H, Phipps J, Saroufeem R, Southard J, Elson DS, Marcu L - Opt Express (2011)

Bottom Line: Distinct compositions of excised human atherosclerotic aorta were clearly discriminated over scanning lengths of several centimeters based on fluorescence lifetime and the intensity ratio between 390 and 452 nm.Operation of STWRFS blood flow was further validated in pig femoral arteries in vivo using a single-fiber probe integrated with an ultrasound imaging catheter.Current results demonstrate the potential of STWRFS as a tool for real-time optical characterization of arterial tissue composition and for atherosclerosis research and diagnosis.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Engineering, University of California Davis, Davis, CA 95616, USA.

ABSTRACT
Simultaneous time- and wavelength-resolved fluorescence spectroscopy (STWRFS) was developed and tested for the dynamic characterization of atherosclerotic tissue ex vivo and arterial vessels in vivo. Autofluorescence, induced by a 337 nm, 700 ps pulsed laser, was split to three wavelength sub-bands using dichroic filters, with each sub-band coupled into a different length of optical fiber for temporal separation. STWRFS allows for fast recording/analysis (few microseconds) of time-resolved fluorescence emission in these sub-bands and rapid scanning. Distinct compositions of excised human atherosclerotic aorta were clearly discriminated over scanning lengths of several centimeters based on fluorescence lifetime and the intensity ratio between 390 and 452 nm. Operation of STWRFS blood flow was further validated in pig femoral arteries in vivo using a single-fiber probe integrated with an ultrasound imaging catheter. Current results demonstrate the potential of STWRFS as a tool for real-time optical characterization of arterial tissue composition and for atherosclerosis research and diagnosis.

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Related in: MedlinePlus

Example of dynamic scanning of aorta tissue: (a) The aorta was opened and scanned perpendicular to the axial direction. The different regions of tissue were marked with a color bar seen in (a) and (d). (b) STWRFS profiles recorded from a collagen-rich plaque region and the surrounding normal tissue. (c) Fluctuation of the fluorescence intensity along the scanning line from each filter. (d) Variation of fluorescence lifetime and intensity ratios of F1/F2 showing the difference between normal artery and plaque areas. The data shown in (c) and (d) were recorded from a scan measurement crossing the whole tissue from the left to the right shown in (a) with a blue line.
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g003: Example of dynamic scanning of aorta tissue: (a) The aorta was opened and scanned perpendicular to the axial direction. The different regions of tissue were marked with a color bar seen in (a) and (d). (b) STWRFS profiles recorded from a collagen-rich plaque region and the surrounding normal tissue. (c) Fluctuation of the fluorescence intensity along the scanning line from each filter. (d) Variation of fluorescence lifetime and intensity ratios of F1/F2 showing the difference between normal artery and plaque areas. The data shown in (c) and (d) were recorded from a scan measurement crossing the whole tissue from the left to the right shown in (a) with a blue line.

Mentions: Figure 3Fig. 3


Dynamic tissue analysis using time- and wavelength-resolved fluorescence spectroscopy for atherosclerosis diagnosis.

Sun Y, Sun Y, Stephens D, Xie H, Phipps J, Saroufeem R, Southard J, Elson DS, Marcu L - Opt Express (2011)

Example of dynamic scanning of aorta tissue: (a) The aorta was opened and scanned perpendicular to the axial direction. The different regions of tissue were marked with a color bar seen in (a) and (d). (b) STWRFS profiles recorded from a collagen-rich plaque region and the surrounding normal tissue. (c) Fluctuation of the fluorescence intensity along the scanning line from each filter. (d) Variation of fluorescence lifetime and intensity ratios of F1/F2 showing the difference between normal artery and plaque areas. The data shown in (c) and (d) were recorded from a scan measurement crossing the whole tissue from the left to the right shown in (a) with a blue line.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3368314&req=5

g003: Example of dynamic scanning of aorta tissue: (a) The aorta was opened and scanned perpendicular to the axial direction. The different regions of tissue were marked with a color bar seen in (a) and (d). (b) STWRFS profiles recorded from a collagen-rich plaque region and the surrounding normal tissue. (c) Fluctuation of the fluorescence intensity along the scanning line from each filter. (d) Variation of fluorescence lifetime and intensity ratios of F1/F2 showing the difference between normal artery and plaque areas. The data shown in (c) and (d) were recorded from a scan measurement crossing the whole tissue from the left to the right shown in (a) with a blue line.
Mentions: Figure 3Fig. 3

Bottom Line: Distinct compositions of excised human atherosclerotic aorta were clearly discriminated over scanning lengths of several centimeters based on fluorescence lifetime and the intensity ratio between 390 and 452 nm.Operation of STWRFS blood flow was further validated in pig femoral arteries in vivo using a single-fiber probe integrated with an ultrasound imaging catheter.Current results demonstrate the potential of STWRFS as a tool for real-time optical characterization of arterial tissue composition and for atherosclerosis research and diagnosis.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Engineering, University of California Davis, Davis, CA 95616, USA.

ABSTRACT
Simultaneous time- and wavelength-resolved fluorescence spectroscopy (STWRFS) was developed and tested for the dynamic characterization of atherosclerotic tissue ex vivo and arterial vessels in vivo. Autofluorescence, induced by a 337 nm, 700 ps pulsed laser, was split to three wavelength sub-bands using dichroic filters, with each sub-band coupled into a different length of optical fiber for temporal separation. STWRFS allows for fast recording/analysis (few microseconds) of time-resolved fluorescence emission in these sub-bands and rapid scanning. Distinct compositions of excised human atherosclerotic aorta were clearly discriminated over scanning lengths of several centimeters based on fluorescence lifetime and the intensity ratio between 390 and 452 nm. Operation of STWRFS blood flow was further validated in pig femoral arteries in vivo using a single-fiber probe integrated with an ultrasound imaging catheter. Current results demonstrate the potential of STWRFS as a tool for real-time optical characterization of arterial tissue composition and for atherosclerosis research and diagnosis.

Show MeSH
Related in: MedlinePlus