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First report of the exotic blue land planarian, Caenoplana coerulea (Platyhelminthes, Geoplanidae), on Menorca (Balearic Islands, Spain).

Breugelmans K, Cardona JQ, Artois T, Jordaens K, Backeljau T - Zookeys (2012)

Bottom Line: Their external morphology suggested that both specimens belonged to the invasive blue planarian Caenoplana coerulea, a species which is native to eastern Australia.Sequence data of a fragment of the mitochondrial cytochrome c oxidase subunit I (COI) and of the entire 18S ribosomal RNA confirm its identification.This is one of the first records of the species in Europe where it has only been found in one locality in the United Kingdom, France and NE Spain.

View Article: PubMed Central - PubMed

Affiliation: Royal Belgian Institute of Natural Sciences, Vautierstraat 29, B-1000 Brussels, Belgium.

ABSTRACT
In April 2009 two specimens of a terrestrial flatworm were collected from under a rock in an orchard at Ciutadella de Menorca on the easternmost Balearic island of Menorca (Spain). Their external morphology suggested that both specimens belonged to the invasive blue planarian Caenoplana coerulea, a species which is native to eastern Australia. Sequence data of a fragment of the mitochondrial cytochrome c oxidase subunit I (COI) and of the entire 18S ribosomal RNA confirm its identification. This is one of the first records of the species in Europe where it has only been found in one locality in the United Kingdom, France and NE Spain.

No MeSH data available.


Neighbor-Joining and ML tree of the 1765 bp dataset of the nuclear 18S rDNA gene. The clones (cl) of the Menorcan specimens ‘1957’ and ‘1958’ are indicated with an asterisk. Bootstrap values ≥ 70% for the NJ and ML trees are given as NJ/ML and are shown at the nodes. – indicates that the node was not supported by the analysis. Note that the clades of the type I and type II 18S rRNA variants are not supported.
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Figure 4: Neighbor-Joining and ML tree of the 1765 bp dataset of the nuclear 18S rDNA gene. The clones (cl) of the Menorcan specimens ‘1957’ and ‘1958’ are indicated with an asterisk. Bootstrap values ≥ 70% for the NJ and ML trees are given as NJ/ML and are shown at the nodes. – indicates that the node was not supported by the analysis. Note that the clades of the type I and type II 18S rRNA variants are not supported.

Mentions: The dorsal dark blue ground-colour with a thin median dorsal stripe, the intense blue colour of the ventral side, and eyes that are arranged in a single row around the anterior tip and which do not extend dorsally, suggest that the two specimens belong to the species of blue land planarian, Caenoplana coerulea Moseley, 1877 (Geoplanidae). This is corroborated by our phylogenetic analysis of the COI and 18S rDNA genes. Both individuals had the same COI haplotype; as in other triclads, there were two different intra-individual types of 18S rDNA (Carranza et al. 1996, 1999). We found five type I and eight type II 18S rDNA variants. Figures 3–4 show the phylogenetic trees inferred from the COI and 18S rDNA data, respectively. The COI haplotype of the Menorcan specimens clustered with strong support with a haplotype of Caenoplana coerulea from the UK (GenBank accession number DQ666030), from which it differed by only one, ambiguous position (i.e. a G for DQ666030, while ‘N’ for the Menorcan haplotype). The mean P-distance between the COI haplotype from Menorca and the other Caenoplana coerulea haplotypes was 0.10 ± 0.02, whereas the P-distance with other Geoplanid species was higher (0.16 ± 0.03) and comparable to what we found among Geoplanidae taxa (0.17 ± 0.03). The 18S rDNA type I sequences from the Menorcan specimens formed a strongly supported clade with Caenoplana coeruleaAF033040 (from the UK) (mean P-distance: 0.008 ± 0.002), whereas those of 18S rDNA type II formed a strongly supported clade with Caenoplana sp.1 AF048765 (unknown origin) and Caenoplana sp. ‘Armidale’ AJ270156 (from Australia) (mean P-distance: 0.003 ± 0.001). The mean P-distance between the Menorcan type I and type II sequences and sequences from the other geoplanid species was substantially higher, viz. 0.019 ± 0.003 and 0.058 ± 0.005, respectively.


First report of the exotic blue land planarian, Caenoplana coerulea (Platyhelminthes, Geoplanidae), on Menorca (Balearic Islands, Spain).

Breugelmans K, Cardona JQ, Artois T, Jordaens K, Backeljau T - Zookeys (2012)

Neighbor-Joining and ML tree of the 1765 bp dataset of the nuclear 18S rDNA gene. The clones (cl) of the Menorcan specimens ‘1957’ and ‘1958’ are indicated with an asterisk. Bootstrap values ≥ 70% for the NJ and ML trees are given as NJ/ML and are shown at the nodes. – indicates that the node was not supported by the analysis. Note that the clades of the type I and type II 18S rRNA variants are not supported.
© Copyright Policy - creative-commons-attribution
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3368283&req=5

Figure 4: Neighbor-Joining and ML tree of the 1765 bp dataset of the nuclear 18S rDNA gene. The clones (cl) of the Menorcan specimens ‘1957’ and ‘1958’ are indicated with an asterisk. Bootstrap values ≥ 70% for the NJ and ML trees are given as NJ/ML and are shown at the nodes. – indicates that the node was not supported by the analysis. Note that the clades of the type I and type II 18S rRNA variants are not supported.
Mentions: The dorsal dark blue ground-colour with a thin median dorsal stripe, the intense blue colour of the ventral side, and eyes that are arranged in a single row around the anterior tip and which do not extend dorsally, suggest that the two specimens belong to the species of blue land planarian, Caenoplana coerulea Moseley, 1877 (Geoplanidae). This is corroborated by our phylogenetic analysis of the COI and 18S rDNA genes. Both individuals had the same COI haplotype; as in other triclads, there were two different intra-individual types of 18S rDNA (Carranza et al. 1996, 1999). We found five type I and eight type II 18S rDNA variants. Figures 3–4 show the phylogenetic trees inferred from the COI and 18S rDNA data, respectively. The COI haplotype of the Menorcan specimens clustered with strong support with a haplotype of Caenoplana coerulea from the UK (GenBank accession number DQ666030), from which it differed by only one, ambiguous position (i.e. a G for DQ666030, while ‘N’ for the Menorcan haplotype). The mean P-distance between the COI haplotype from Menorca and the other Caenoplana coerulea haplotypes was 0.10 ± 0.02, whereas the P-distance with other Geoplanid species was higher (0.16 ± 0.03) and comparable to what we found among Geoplanidae taxa (0.17 ± 0.03). The 18S rDNA type I sequences from the Menorcan specimens formed a strongly supported clade with Caenoplana coeruleaAF033040 (from the UK) (mean P-distance: 0.008 ± 0.002), whereas those of 18S rDNA type II formed a strongly supported clade with Caenoplana sp.1 AF048765 (unknown origin) and Caenoplana sp. ‘Armidale’ AJ270156 (from Australia) (mean P-distance: 0.003 ± 0.001). The mean P-distance between the Menorcan type I and type II sequences and sequences from the other geoplanid species was substantially higher, viz. 0.019 ± 0.003 and 0.058 ± 0.005, respectively.

Bottom Line: Their external morphology suggested that both specimens belonged to the invasive blue planarian Caenoplana coerulea, a species which is native to eastern Australia.Sequence data of a fragment of the mitochondrial cytochrome c oxidase subunit I (COI) and of the entire 18S ribosomal RNA confirm its identification.This is one of the first records of the species in Europe where it has only been found in one locality in the United Kingdom, France and NE Spain.

View Article: PubMed Central - PubMed

Affiliation: Royal Belgian Institute of Natural Sciences, Vautierstraat 29, B-1000 Brussels, Belgium.

ABSTRACT
In April 2009 two specimens of a terrestrial flatworm were collected from under a rock in an orchard at Ciutadella de Menorca on the easternmost Balearic island of Menorca (Spain). Their external morphology suggested that both specimens belonged to the invasive blue planarian Caenoplana coerulea, a species which is native to eastern Australia. Sequence data of a fragment of the mitochondrial cytochrome c oxidase subunit I (COI) and of the entire 18S ribosomal RNA confirm its identification. This is one of the first records of the species in Europe where it has only been found in one locality in the United Kingdom, France and NE Spain.

No MeSH data available.