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Taxonomy and species-groups of the subgenus Crematogaster ( Orthocrema) in the Malagasy region (Hymenoptera, Formicidae).

Blaimer BB - Zookeys (2012)

Bottom Line: Within the Malagasy Orthocrema, three distinct phylogenetic lineages are suggested by molecular and morphological data.Newly defined monophyletic species-groups are thus the Crematogaster madecassa-group (Crematogaster madecassa, Crematogaster telolafy and Crematogaster razana) and the Crematogaster volamena-group (Crematogaster volamena and Crematogaster mpanjono); Crematogaster rasoherinae represents an isolated lineage in the Malagasy region and its closest relatives remain unclear.Other interesting biological findings are the presence of an intermediate caste between workers and queens in Crematogaster rasoherinae and Crematogaster madecassa, and unusually large workers in Crematogaster volamena resembling a major caste.

View Article: PubMed Central - PubMed

Affiliation: Department of Entomology, University of California-Davis, One Shields Ave, Davis, CA 95616, USA.

ABSTRACT
The species-level taxonomy of the subgenus Crematogaster (Orthocrema) in the Malagasy region is evaluated with both morphological data from worker and queen ants, and genetic data from three nuclear markers (long wavelength rhodopsin, arginine kinase and carbamoylphosphate synthase). These two types of data support the existence of six Orthocrema species: Crematogaster madecassa Emery, Crematogaster rasoherinae Forel, Crematogaster telolafysp. n., Crematogaster razanasp. n., Crematogaster volamenasp. n. and Crematogaster mpanjonosp. n.. Two new synonyms of Crematogaster rasoherinae Forel are recognized, Crematogaster rasoherinae brunneola Emery, syn.n. and Crematogaster voeltzkowi Forel, syn. n., as these were not supported as distinct taxa by the data. A neotype is designated for Crematogaster rasoherinae; lectotypes are designated for Crematogaster madecassa, Crematogaster rasoherinae brunneola and Crematogaster voeltzkowi. Species descriptions, images, distribution maps and identification keys based on worker and queen ants are given for all six species. A diagnosis of the subgenus Orthocrema in the Malagasy region is presented for both workers and queens. Within the Malagasy Orthocrema, three distinct phylogenetic lineages are suggested by molecular and morphological data. Newly defined monophyletic species-groups are thus the Crematogaster madecassa-group (Crematogaster madecassa, Crematogaster telolafy and Crematogaster razana) and the Crematogaster volamena-group (Crematogaster volamena and Crematogaster mpanjono); Crematogaster rasoherinae represents an isolated lineage in the Malagasy region and its closest relatives remain unclear. Other interesting biological findings are the presence of an intermediate caste between workers and queens in Crematogaster rasoherinae and Crematogaster madecassa, and unusually large workers in Crematogaster volamena resembling a major caste.

No MeSH data available.


Related in: MedlinePlus

Species phylogeny of Malagasy Crematogaster (Orthocrema). A Results of Bayesian inference summarized as consensus tree in MrBayes. Support values on branches represent posterior probabilities; scalebar shows nucleotide changes per base pair. Newly defined species-groups, and the specimen representing former Crematogaster voeltzkowi are indicated. Outgroup species are marked by blue font B ML-consensus tree with bootstrap support values obtained from analysis with 100 bootstrap replicates in GARLI 2.0.
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Figure 1: Species phylogeny of Malagasy Crematogaster (Orthocrema). A Results of Bayesian inference summarized as consensus tree in MrBayes. Support values on branches represent posterior probabilities; scalebar shows nucleotide changes per base pair. Newly defined species-groups, and the specimen representing former Crematogaster voeltzkowi are indicated. Outgroup species are marked by blue font B ML-consensus tree with bootstrap support values obtained from analysis with 100 bootstrap replicates in GARLI 2.0.

Mentions: From these 17 specimens, DNA was extracted from either entire worker adults or pupae using a DNeasy Tissue Kit (Qiagen Inc., Valencia, California, U.S.A.), following the manufacturer’s protocol but eluting the extract in sterilized water rather than the supplied buffer and at half the suggested volume. I used either a non-destructive method (cuticle of ant pierced prior to extraction, mostly used for adults), enabling me to retain and re-mount voucher specimens after extractions, or a destructive technique (entire ant pulverized, mostly used for pupae) in cases where multiple individuals from the same colony series were available. Three nuclear protein-coding genes were amplified:long wavelength rhodopsin (LW Rh, 856bp exon /255bp intron), arginine kinase (ArgK, 388bp exon/177bp intron) and carbamoylphosphate synthase (CAD, 529bp exon/252bp intron). The sequence lengths given here refer to the aligned sequence data included in phylogenetic inference and add up to a total of 2457bp. The three amplified genes are widely used for phylogenetic inference in ants and primers are available (Ward and Downie 2005; Brady et al. 2006; Moreau et al. 2006; Ward et al. 2010; Blaimer in prep.), and their usefulness in phylogenetic inference between closely related species has been demonstrated (Lucky 2011; Blaimer 2012). Amplifications were performed using standard PCR methods outlined in Ward and Downie (2005) and sequencing reactions were analyzed on an ABI 3730 Capillary Electrophoresis Genetic Analyzer with ABI BigDye Terminator v3.1 Cycle Sequencing chemistry (Applied Biosystems Inc., Foster City, CA). All sequences have been deposited in GenBank, with accession numbers listed in Table 1; the data matrix and tree used to create Fig. 1 have further been deposited in TreeBase (ID 12240; available at: http://purl.org/phylo/treebase/phylows/study/TB2:S12240).


Taxonomy and species-groups of the subgenus Crematogaster ( Orthocrema) in the Malagasy region (Hymenoptera, Formicidae).

Blaimer BB - Zookeys (2012)

Species phylogeny of Malagasy Crematogaster (Orthocrema). A Results of Bayesian inference summarized as consensus tree in MrBayes. Support values on branches represent posterior probabilities; scalebar shows nucleotide changes per base pair. Newly defined species-groups, and the specimen representing former Crematogaster voeltzkowi are indicated. Outgroup species are marked by blue font B ML-consensus tree with bootstrap support values obtained from analysis with 100 bootstrap replicates in GARLI 2.0.
© Copyright Policy - creative-commons-attribution
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3368281&req=5

Figure 1: Species phylogeny of Malagasy Crematogaster (Orthocrema). A Results of Bayesian inference summarized as consensus tree in MrBayes. Support values on branches represent posterior probabilities; scalebar shows nucleotide changes per base pair. Newly defined species-groups, and the specimen representing former Crematogaster voeltzkowi are indicated. Outgroup species are marked by blue font B ML-consensus tree with bootstrap support values obtained from analysis with 100 bootstrap replicates in GARLI 2.0.
Mentions: From these 17 specimens, DNA was extracted from either entire worker adults or pupae using a DNeasy Tissue Kit (Qiagen Inc., Valencia, California, U.S.A.), following the manufacturer’s protocol but eluting the extract in sterilized water rather than the supplied buffer and at half the suggested volume. I used either a non-destructive method (cuticle of ant pierced prior to extraction, mostly used for adults), enabling me to retain and re-mount voucher specimens after extractions, or a destructive technique (entire ant pulverized, mostly used for pupae) in cases where multiple individuals from the same colony series were available. Three nuclear protein-coding genes were amplified:long wavelength rhodopsin (LW Rh, 856bp exon /255bp intron), arginine kinase (ArgK, 388bp exon/177bp intron) and carbamoylphosphate synthase (CAD, 529bp exon/252bp intron). The sequence lengths given here refer to the aligned sequence data included in phylogenetic inference and add up to a total of 2457bp. The three amplified genes are widely used for phylogenetic inference in ants and primers are available (Ward and Downie 2005; Brady et al. 2006; Moreau et al. 2006; Ward et al. 2010; Blaimer in prep.), and their usefulness in phylogenetic inference between closely related species has been demonstrated (Lucky 2011; Blaimer 2012). Amplifications were performed using standard PCR methods outlined in Ward and Downie (2005) and sequencing reactions were analyzed on an ABI 3730 Capillary Electrophoresis Genetic Analyzer with ABI BigDye Terminator v3.1 Cycle Sequencing chemistry (Applied Biosystems Inc., Foster City, CA). All sequences have been deposited in GenBank, with accession numbers listed in Table 1; the data matrix and tree used to create Fig. 1 have further been deposited in TreeBase (ID 12240; available at: http://purl.org/phylo/treebase/phylows/study/TB2:S12240).

Bottom Line: Within the Malagasy Orthocrema, three distinct phylogenetic lineages are suggested by molecular and morphological data.Newly defined monophyletic species-groups are thus the Crematogaster madecassa-group (Crematogaster madecassa, Crematogaster telolafy and Crematogaster razana) and the Crematogaster volamena-group (Crematogaster volamena and Crematogaster mpanjono); Crematogaster rasoherinae represents an isolated lineage in the Malagasy region and its closest relatives remain unclear.Other interesting biological findings are the presence of an intermediate caste between workers and queens in Crematogaster rasoherinae and Crematogaster madecassa, and unusually large workers in Crematogaster volamena resembling a major caste.

View Article: PubMed Central - PubMed

Affiliation: Department of Entomology, University of California-Davis, One Shields Ave, Davis, CA 95616, USA.

ABSTRACT
The species-level taxonomy of the subgenus Crematogaster (Orthocrema) in the Malagasy region is evaluated with both morphological data from worker and queen ants, and genetic data from three nuclear markers (long wavelength rhodopsin, arginine kinase and carbamoylphosphate synthase). These two types of data support the existence of six Orthocrema species: Crematogaster madecassa Emery, Crematogaster rasoherinae Forel, Crematogaster telolafysp. n., Crematogaster razanasp. n., Crematogaster volamenasp. n. and Crematogaster mpanjonosp. n.. Two new synonyms of Crematogaster rasoherinae Forel are recognized, Crematogaster rasoherinae brunneola Emery, syn.n. and Crematogaster voeltzkowi Forel, syn. n., as these were not supported as distinct taxa by the data. A neotype is designated for Crematogaster rasoherinae; lectotypes are designated for Crematogaster madecassa, Crematogaster rasoherinae brunneola and Crematogaster voeltzkowi. Species descriptions, images, distribution maps and identification keys based on worker and queen ants are given for all six species. A diagnosis of the subgenus Orthocrema in the Malagasy region is presented for both workers and queens. Within the Malagasy Orthocrema, three distinct phylogenetic lineages are suggested by molecular and morphological data. Newly defined monophyletic species-groups are thus the Crematogaster madecassa-group (Crematogaster madecassa, Crematogaster telolafy and Crematogaster razana) and the Crematogaster volamena-group (Crematogaster volamena and Crematogaster mpanjono); Crematogaster rasoherinae represents an isolated lineage in the Malagasy region and its closest relatives remain unclear. Other interesting biological findings are the presence of an intermediate caste between workers and queens in Crematogaster rasoherinae and Crematogaster madecassa, and unusually large workers in Crematogaster volamena resembling a major caste.

No MeSH data available.


Related in: MedlinePlus