Early B-cell factors 2 and 3 (EBF2/3) regulate early migration of Cajal-Retzius cells from the cortical hem.
Bottom Line: Here, we show that Ebf transcription factors are expressed in forebrain signalling centres-the septum, cortical hem and the pallial-subpallial boundary-known to generate CR cells.Accordingly, using in vitro preparations, we demonstrated that both Ebf2 and Ebf3, singly or together, control the migration of CR cells arising in the cortical hem.These findings provide evidence that Ebfs directly regulate CR cell development.
Affiliation: Department of Cell and Developmental Biology, University College London, UK.Show MeSH
Mentions: The majority of Mock treated cells preferred PPL/LN to PLL stripes (Fig. 8A; D: LN/PLL 952 ± 79, PLL 657 ± 29; n = 3; p < 0.01). Similarly, cells overexpressing Ebf2/3-flag were mostly found on PPL/LN stripes (Fig. 8B compared to A; D: LN/PLL 1150 ± 82, PLL 305 ± 66; n = 3; p < 0.001), but in this case the amount of cells attached to LN/PLL was significantly higher when compared with the Mock treated samples, suggesting that they may require less time to migrate and attach to LN/PLL stripes (p < 0.001). Cells treated with shEbf2/3 did not show any preference but, instead, they were randomly positioned in the chamber suggesting that they were unable to respond to the chemoattractant or requiring more time to reach the preferred stripe (Fig. 8C compared to A and B; D: LN/PLL 566 ± 88, PLL 735 ± 67; n = 3; p < 0.001 when compared to Mock and Ebf2/3-flag). Interestingly, cells transfected with single plasmids showed less severe phenotype (data not shown), indicating a redundant role for Ebf2 and Ebf3 in CH-derived CR cell migration.
Affiliation: Department of Cell and Developmental Biology, University College London, UK.