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Early B-cell factors 2 and 3 (EBF2/3) regulate early migration of Cajal-Retzius cells from the cortical hem.

Chiara F, Badaloni A, Croci L, Yeh ML, Cariboni A, Hoerder-Suabedissen A, Consalez GG, Eickholt B, Shimogori T, Parnavelas JG, Rakić S - Dev. Biol. (2012)

Bottom Line: Here, we show that Ebf transcription factors are expressed in forebrain signalling centres-the septum, cortical hem and the pallial-subpallial boundary-known to generate CR cells.Accordingly, using in vitro preparations, we demonstrated that both Ebf2 and Ebf3, singly or together, control the migration of CR cells arising in the cortical hem.These findings provide evidence that Ebfs directly regulate CR cell development.

View Article: PubMed Central - PubMed

Affiliation: Department of Cell and Developmental Biology, University College London, UK.

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Ebfs overexpression and downregulation affect the migration of CR cells in vitro. Mock-treated cells prefer LN/PLL stripes compared to PLL only (Mock, A and D). Ebf2/3-flag overexpression increase the ability of CR cells to migrate towards LN stripes (B compared to A; D: Ebf2/3-flag, p < 0.001 LN/PLL compared to PLL; Ebf2/3-flag group compared to Mock group, p < 0.001, solid line). Ebf2/3 downregulation decreases, instead, the ability of CR cells to migrate towards LN (C compared to A and B; D: shEbf2/3-GFP group compared to Mock, p < 0.01, solid line, or compared to Ebf2/3-flag, p < 0.001, dotted line). DIV: days in vitro, LN: laminin, PLL: poly-l-lysin. Scale bar: 25 μm.
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f0040: Ebfs overexpression and downregulation affect the migration of CR cells in vitro. Mock-treated cells prefer LN/PLL stripes compared to PLL only (Mock, A and D). Ebf2/3-flag overexpression increase the ability of CR cells to migrate towards LN stripes (B compared to A; D: Ebf2/3-flag, p < 0.001 LN/PLL compared to PLL; Ebf2/3-flag group compared to Mock group, p < 0.001, solid line). Ebf2/3 downregulation decreases, instead, the ability of CR cells to migrate towards LN (C compared to A and B; D: shEbf2/3-GFP group compared to Mock, p < 0.01, solid line, or compared to Ebf2/3-flag, p < 0.001, dotted line). DIV: days in vitro, LN: laminin, PLL: poly-l-lysin. Scale bar: 25 μm.

Mentions: The majority of Mock treated cells preferred PPL/LN to PLL stripes (Fig. 8A; D: LN/PLL 952 ± 79, PLL 657 ± 29; n = 3; p < 0.01). Similarly, cells overexpressing Ebf2/3-flag were mostly found on PPL/LN stripes (Fig. 8B compared to A; D: LN/PLL 1150 ± 82, PLL 305 ± 66; n = 3; p < 0.001), but in this case the amount of cells attached to LN/PLL was significantly higher when compared with the Mock treated samples, suggesting that they may require less time to migrate and attach to LN/PLL stripes (p < 0.001). Cells treated with shEbf2/3 did not show any preference but, instead, they were randomly positioned in the chamber suggesting that they were unable to respond to the chemoattractant or requiring more time to reach the preferred stripe (Fig. 8C compared to A and B; D: LN/PLL 566 ± 88, PLL 735 ± 67; n = 3; p < 0.001 when compared to Mock and Ebf2/3-flag). Interestingly, cells transfected with single plasmids showed less severe phenotype (data not shown), indicating a redundant role for Ebf2 and Ebf3 in CH-derived CR cell migration.


Early B-cell factors 2 and 3 (EBF2/3) regulate early migration of Cajal-Retzius cells from the cortical hem.

Chiara F, Badaloni A, Croci L, Yeh ML, Cariboni A, Hoerder-Suabedissen A, Consalez GG, Eickholt B, Shimogori T, Parnavelas JG, Rakić S - Dev. Biol. (2012)

Ebfs overexpression and downregulation affect the migration of CR cells in vitro. Mock-treated cells prefer LN/PLL stripes compared to PLL only (Mock, A and D). Ebf2/3-flag overexpression increase the ability of CR cells to migrate towards LN stripes (B compared to A; D: Ebf2/3-flag, p < 0.001 LN/PLL compared to PLL; Ebf2/3-flag group compared to Mock group, p < 0.001, solid line). Ebf2/3 downregulation decreases, instead, the ability of CR cells to migrate towards LN (C compared to A and B; D: shEbf2/3-GFP group compared to Mock, p < 0.01, solid line, or compared to Ebf2/3-flag, p < 0.001, dotted line). DIV: days in vitro, LN: laminin, PLL: poly-l-lysin. Scale bar: 25 μm.
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3368273&req=5

f0040: Ebfs overexpression and downregulation affect the migration of CR cells in vitro. Mock-treated cells prefer LN/PLL stripes compared to PLL only (Mock, A and D). Ebf2/3-flag overexpression increase the ability of CR cells to migrate towards LN stripes (B compared to A; D: Ebf2/3-flag, p < 0.001 LN/PLL compared to PLL; Ebf2/3-flag group compared to Mock group, p < 0.001, solid line). Ebf2/3 downregulation decreases, instead, the ability of CR cells to migrate towards LN (C compared to A and B; D: shEbf2/3-GFP group compared to Mock, p < 0.01, solid line, or compared to Ebf2/3-flag, p < 0.001, dotted line). DIV: days in vitro, LN: laminin, PLL: poly-l-lysin. Scale bar: 25 μm.
Mentions: The majority of Mock treated cells preferred PPL/LN to PLL stripes (Fig. 8A; D: LN/PLL 952 ± 79, PLL 657 ± 29; n = 3; p < 0.01). Similarly, cells overexpressing Ebf2/3-flag were mostly found on PPL/LN stripes (Fig. 8B compared to A; D: LN/PLL 1150 ± 82, PLL 305 ± 66; n = 3; p < 0.001), but in this case the amount of cells attached to LN/PLL was significantly higher when compared with the Mock treated samples, suggesting that they may require less time to migrate and attach to LN/PLL stripes (p < 0.001). Cells treated with shEbf2/3 did not show any preference but, instead, they were randomly positioned in the chamber suggesting that they were unable to respond to the chemoattractant or requiring more time to reach the preferred stripe (Fig. 8C compared to A and B; D: LN/PLL 566 ± 88, PLL 735 ± 67; n = 3; p < 0.001 when compared to Mock and Ebf2/3-flag). Interestingly, cells transfected with single plasmids showed less severe phenotype (data not shown), indicating a redundant role for Ebf2 and Ebf3 in CH-derived CR cell migration.

Bottom Line: Here, we show that Ebf transcription factors are expressed in forebrain signalling centres-the septum, cortical hem and the pallial-subpallial boundary-known to generate CR cells.Accordingly, using in vitro preparations, we demonstrated that both Ebf2 and Ebf3, singly or together, control the migration of CR cells arising in the cortical hem.These findings provide evidence that Ebfs directly regulate CR cell development.

View Article: PubMed Central - PubMed

Affiliation: Department of Cell and Developmental Biology, University College London, UK.

Show MeSH