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Early B-cell factors 2 and 3 (EBF2/3) regulate early migration of Cajal-Retzius cells from the cortical hem.

Chiara F, Badaloni A, Croci L, Yeh ML, Cariboni A, Hoerder-Suabedissen A, Consalez GG, Eickholt B, Shimogori T, Parnavelas JG, Rakić S - Dev. Biol. (2012)

Bottom Line: Here, we show that Ebf transcription factors are expressed in forebrain signalling centres-the septum, cortical hem and the pallial-subpallial boundary-known to generate CR cells.Accordingly, using in vitro preparations, we demonstrated that both Ebf2 and Ebf3, singly or together, control the migration of CR cells arising in the cortical hem.These findings provide evidence that Ebfs directly regulate CR cell development.

View Article: PubMed Central - PubMed

Affiliation: Department of Cell and Developmental Biology, University College London, UK.

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Ebfs expression in the developing forebrain. In situ hybridization shows that, at E12.5, Ebf1, Ebf2 and Ebf3 are expressed in similar territories (A–C). Arrows point to CH (A–C) and arrowhead to lateral PPL (C) indicating cells migrating from the CH and PSPB, respectively. Ebf3 is detected in the PSPB (C′), whereas Ebf1 in the striatum, but not in the PSPB (A′; circles delineate the St and PSPB). Ebf2 and Ebf3 are expressed in the septum (D–F). At E15.5, Ebf1 and Ebf2 are downregulated in the MZ and CH (G,H), whereas Ebf3 is still detected in the CH, MZ and hippocampus (I; arrow and arrowhead, respectively). CH: cortical hem, PCx: piriform cortex, PPL: preplate, PSPB: pallial subpallial boundary, Sp: septum, St: striatum. Scale bars: (A–C; A′–C′) 100 μm, (G–I) 200 μm.
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f0025: Ebfs expression in the developing forebrain. In situ hybridization shows that, at E12.5, Ebf1, Ebf2 and Ebf3 are expressed in similar territories (A–C). Arrows point to CH (A–C) and arrowhead to lateral PPL (C) indicating cells migrating from the CH and PSPB, respectively. Ebf3 is detected in the PSPB (C′), whereas Ebf1 in the striatum, but not in the PSPB (A′; circles delineate the St and PSPB). Ebf2 and Ebf3 are expressed in the septum (D–F). At E15.5, Ebf1 and Ebf2 are downregulated in the MZ and CH (G,H), whereas Ebf3 is still detected in the CH, MZ and hippocampus (I; arrow and arrowhead, respectively). CH: cortical hem, PCx: piriform cortex, PPL: preplate, PSPB: pallial subpallial boundary, Sp: septum, St: striatum. Scale bars: (A–C; A′–C′) 100 μm, (G–I) 200 μm.

Mentions: Previous studies have implied that Ebfs are expressed in overlapping territories of the developing forebrain and may have redundant roles (Garel et al., 1997). To identify the pattern of expression of Ebf1 and Ebf3, forebrains of mice of different ages were analysed with in situ hybridization. Ebf1 and Ebf3 were detected from E10.5 to E15.5, when they were downregulated in a similar fashion to Ebf2 (Figs. 1J–L). At E12.5, Ebf3 expression was similar to that of Ebf2 in the CH and PPL (Figs. 5B,C), however Ebf3 was broadly expressed in the CH, whereas Ebf2 was restricted to a subdomain (Fig. 5C compared to B). Ebf1 was strongly expressed in the PPL, but was almost absent in the CH (Fig. 5A). Additionally, Ebf3 was detected in the PSPB (Fig. 5C′). In the septum, Ebf3 was expressed broadly along the midline, whereas Ebf2 was confined dorsally (Figs. 5F,E), while no Ebf1 expression was found (Fig. 5D). At E15.5, Ebf1 was downregulated in the pallium (Fig. 5G), similar to Ebf2 (Fig. 5H), but not in the striatum (Garel et al., 1997). Ebf3 was still detected in the CH and MZ (Fig. 5I) and persisted in these areas after birth (data not shown). Ebfs are transiently expressed in the same forebrain structures, specifically in the CH and septum, and PPL/MZ, the sites of origin and migration of CR cells, respectively. Thus, these genes could act together to regulate CR cell development.


Early B-cell factors 2 and 3 (EBF2/3) regulate early migration of Cajal-Retzius cells from the cortical hem.

Chiara F, Badaloni A, Croci L, Yeh ML, Cariboni A, Hoerder-Suabedissen A, Consalez GG, Eickholt B, Shimogori T, Parnavelas JG, Rakić S - Dev. Biol. (2012)

Ebfs expression in the developing forebrain. In situ hybridization shows that, at E12.5, Ebf1, Ebf2 and Ebf3 are expressed in similar territories (A–C). Arrows point to CH (A–C) and arrowhead to lateral PPL (C) indicating cells migrating from the CH and PSPB, respectively. Ebf3 is detected in the PSPB (C′), whereas Ebf1 in the striatum, but not in the PSPB (A′; circles delineate the St and PSPB). Ebf2 and Ebf3 are expressed in the septum (D–F). At E15.5, Ebf1 and Ebf2 are downregulated in the MZ and CH (G,H), whereas Ebf3 is still detected in the CH, MZ and hippocampus (I; arrow and arrowhead, respectively). CH: cortical hem, PCx: piriform cortex, PPL: preplate, PSPB: pallial subpallial boundary, Sp: septum, St: striatum. Scale bars: (A–C; A′–C′) 100 μm, (G–I) 200 μm.
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Related In: Results  -  Collection

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f0025: Ebfs expression in the developing forebrain. In situ hybridization shows that, at E12.5, Ebf1, Ebf2 and Ebf3 are expressed in similar territories (A–C). Arrows point to CH (A–C) and arrowhead to lateral PPL (C) indicating cells migrating from the CH and PSPB, respectively. Ebf3 is detected in the PSPB (C′), whereas Ebf1 in the striatum, but not in the PSPB (A′; circles delineate the St and PSPB). Ebf2 and Ebf3 are expressed in the septum (D–F). At E15.5, Ebf1 and Ebf2 are downregulated in the MZ and CH (G,H), whereas Ebf3 is still detected in the CH, MZ and hippocampus (I; arrow and arrowhead, respectively). CH: cortical hem, PCx: piriform cortex, PPL: preplate, PSPB: pallial subpallial boundary, Sp: septum, St: striatum. Scale bars: (A–C; A′–C′) 100 μm, (G–I) 200 μm.
Mentions: Previous studies have implied that Ebfs are expressed in overlapping territories of the developing forebrain and may have redundant roles (Garel et al., 1997). To identify the pattern of expression of Ebf1 and Ebf3, forebrains of mice of different ages were analysed with in situ hybridization. Ebf1 and Ebf3 were detected from E10.5 to E15.5, when they were downregulated in a similar fashion to Ebf2 (Figs. 1J–L). At E12.5, Ebf3 expression was similar to that of Ebf2 in the CH and PPL (Figs. 5B,C), however Ebf3 was broadly expressed in the CH, whereas Ebf2 was restricted to a subdomain (Fig. 5C compared to B). Ebf1 was strongly expressed in the PPL, but was almost absent in the CH (Fig. 5A). Additionally, Ebf3 was detected in the PSPB (Fig. 5C′). In the septum, Ebf3 was expressed broadly along the midline, whereas Ebf2 was confined dorsally (Figs. 5F,E), while no Ebf1 expression was found (Fig. 5D). At E15.5, Ebf1 was downregulated in the pallium (Fig. 5G), similar to Ebf2 (Fig. 5H), but not in the striatum (Garel et al., 1997). Ebf3 was still detected in the CH and MZ (Fig. 5I) and persisted in these areas after birth (data not shown). Ebfs are transiently expressed in the same forebrain structures, specifically in the CH and septum, and PPL/MZ, the sites of origin and migration of CR cells, respectively. Thus, these genes could act together to regulate CR cell development.

Bottom Line: Here, we show that Ebf transcription factors are expressed in forebrain signalling centres-the septum, cortical hem and the pallial-subpallial boundary-known to generate CR cells.Accordingly, using in vitro preparations, we demonstrated that both Ebf2 and Ebf3, singly or together, control the migration of CR cells arising in the cortical hem.These findings provide evidence that Ebfs directly regulate CR cell development.

View Article: PubMed Central - PubMed

Affiliation: Department of Cell and Developmental Biology, University College London, UK.

Show MeSH