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Early B-cell factors 2 and 3 (EBF2/3) regulate early migration of Cajal-Retzius cells from the cortical hem.

Chiara F, Badaloni A, Croci L, Yeh ML, Cariboni A, Hoerder-Suabedissen A, Consalez GG, Eickholt B, Shimogori T, Parnavelas JG, Rakić S - Dev. Biol. (2012)

Bottom Line: Here, we show that Ebf transcription factors are expressed in forebrain signalling centres-the septum, cortical hem and the pallial-subpallial boundary-known to generate CR cells.Accordingly, using in vitro preparations, we demonstrated that both Ebf2 and Ebf3, singly or together, control the migration of CR cells arising in the cortical hem.These findings provide evidence that Ebfs directly regulate CR cell development.

View Article: PubMed Central - PubMed

Affiliation: Department of Cell and Developmental Biology, University College London, UK.

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Fate mapping of Ebf2+ cells in Ebf2GFPiCre/R26R-YFP. At E13.5 and E16.5, the transgene is expressed in the MZ, SP, CH, H, PCx, Sp and Et, but not in Th and ChP (A–F). Observation at higher magnification reveals that Ebf2 is also expressed in SP cells and neurons in the CP (G, H). At P7, YFP cells are in layer I, layer V and at the bottom of layer VI (SP cells, I). CH: cortical hem, ChP: choroid plexus, CP: cortical plate, Et: epithalamus, H: hippocampus, MZ: marginal zone, SP: subplate, Sp: septum, PCx: piriform cortex, Th: thalamus. Scale bars: (A–F, I) 100 μm, (G) 25 μm, (H) 50 μm.
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f0010: Fate mapping of Ebf2+ cells in Ebf2GFPiCre/R26R-YFP. At E13.5 and E16.5, the transgene is expressed in the MZ, SP, CH, H, PCx, Sp and Et, but not in Th and ChP (A–F). Observation at higher magnification reveals that Ebf2 is also expressed in SP cells and neurons in the CP (G, H). At P7, YFP cells are in layer I, layer V and at the bottom of layer VI (SP cells, I). CH: cortical hem, ChP: choroid plexus, CP: cortical plate, Et: epithalamus, H: hippocampus, MZ: marginal zone, SP: subplate, Sp: septum, PCx: piriform cortex, Th: thalamus. Scale bars: (A–F, I) 100 μm, (G) 25 μm, (H) 50 μm.

Mentions: We then used an Ebf2GFPiCre transgenic line to perform fate-mapping studies on Ebf2+cells. This line was obtained through the integration of the fusion protein GFPiCre (Badaloni A., unpublished) into the first coding exon of the Ebf2 locus in the BAC RP24-283N8 (Fig. S1A). The expression of the transgene faithfully mimicked the expression of the endogenous gene (Figs. S1B–E). In order to permanently label the lineage of Ebf2+ cells, the transgenic line was crossed with the reporter line R26RLacZ (Figs. S1F–H). YFPEbf2 immunohistochemistry, at E13.5 and E16.5, revealed that Ebf2 was expressed along the rostro-caudal axis in forebrain structures such as neocortex, CH, septum, hippocampus and PCx (Figs. 2A–F). Moreover, YFPEbf2-expressing cells in the neocortex were detected in the MZ, cortical plate (CP) and SP (Figs. 2G,H). In the diencephalon, the transgene was expressed in the epithalamus (Figs. 2C,F). No expression was detected in the ChP and thalamic nuclei or axons arising from this area (Figs. 2B,C,E,F). At P7, YFPEbf2 cells in the cerebral cortex were positioned in layers I (former PPL/MZ), IV and V (Figs. 2I, 3M,O), and in the SP (Figs. 2I,3N,N′).


Early B-cell factors 2 and 3 (EBF2/3) regulate early migration of Cajal-Retzius cells from the cortical hem.

Chiara F, Badaloni A, Croci L, Yeh ML, Cariboni A, Hoerder-Suabedissen A, Consalez GG, Eickholt B, Shimogori T, Parnavelas JG, Rakić S - Dev. Biol. (2012)

Fate mapping of Ebf2+ cells in Ebf2GFPiCre/R26R-YFP. At E13.5 and E16.5, the transgene is expressed in the MZ, SP, CH, H, PCx, Sp and Et, but not in Th and ChP (A–F). Observation at higher magnification reveals that Ebf2 is also expressed in SP cells and neurons in the CP (G, H). At P7, YFP cells are in layer I, layer V and at the bottom of layer VI (SP cells, I). CH: cortical hem, ChP: choroid plexus, CP: cortical plate, Et: epithalamus, H: hippocampus, MZ: marginal zone, SP: subplate, Sp: septum, PCx: piriform cortex, Th: thalamus. Scale bars: (A–F, I) 100 μm, (G) 25 μm, (H) 50 μm.
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f0010: Fate mapping of Ebf2+ cells in Ebf2GFPiCre/R26R-YFP. At E13.5 and E16.5, the transgene is expressed in the MZ, SP, CH, H, PCx, Sp and Et, but not in Th and ChP (A–F). Observation at higher magnification reveals that Ebf2 is also expressed in SP cells and neurons in the CP (G, H). At P7, YFP cells are in layer I, layer V and at the bottom of layer VI (SP cells, I). CH: cortical hem, ChP: choroid plexus, CP: cortical plate, Et: epithalamus, H: hippocampus, MZ: marginal zone, SP: subplate, Sp: septum, PCx: piriform cortex, Th: thalamus. Scale bars: (A–F, I) 100 μm, (G) 25 μm, (H) 50 μm.
Mentions: We then used an Ebf2GFPiCre transgenic line to perform fate-mapping studies on Ebf2+cells. This line was obtained through the integration of the fusion protein GFPiCre (Badaloni A., unpublished) into the first coding exon of the Ebf2 locus in the BAC RP24-283N8 (Fig. S1A). The expression of the transgene faithfully mimicked the expression of the endogenous gene (Figs. S1B–E). In order to permanently label the lineage of Ebf2+ cells, the transgenic line was crossed with the reporter line R26RLacZ (Figs. S1F–H). YFPEbf2 immunohistochemistry, at E13.5 and E16.5, revealed that Ebf2 was expressed along the rostro-caudal axis in forebrain structures such as neocortex, CH, septum, hippocampus and PCx (Figs. 2A–F). Moreover, YFPEbf2-expressing cells in the neocortex were detected in the MZ, cortical plate (CP) and SP (Figs. 2G,H). In the diencephalon, the transgene was expressed in the epithalamus (Figs. 2C,F). No expression was detected in the ChP and thalamic nuclei or axons arising from this area (Figs. 2B,C,E,F). At P7, YFPEbf2 cells in the cerebral cortex were positioned in layers I (former PPL/MZ), IV and V (Figs. 2I, 3M,O), and in the SP (Figs. 2I,3N,N′).

Bottom Line: Here, we show that Ebf transcription factors are expressed in forebrain signalling centres-the septum, cortical hem and the pallial-subpallial boundary-known to generate CR cells.Accordingly, using in vitro preparations, we demonstrated that both Ebf2 and Ebf3, singly or together, control the migration of CR cells arising in the cortical hem.These findings provide evidence that Ebfs directly regulate CR cell development.

View Article: PubMed Central - PubMed

Affiliation: Department of Cell and Developmental Biology, University College London, UK.

Show MeSH