The host-seeking inhibitory peptide, Aea-HP-1, is made in the male accessory gland and transferred to the female during copulation.
Bottom Line: The structure of the peptide with its blocked N- and C-termini confers resistance to metabolic inactivation by MAG peptidases; however the peptide persists for less than 2h in the female reproductive tract after copulation.Aea-HP-1 is not a ligand for the mosquito sex peptide/myoinhibitory peptide receptor.A. aegypti often mate close to the host and therefore it is possible that male-derived Aea-HP-1 induces short-term changes to female host-seeking behavior to reduce potentially lethal encounters with hosts soon after insemination.
Affiliation: Faculty of Biological Sciences, University of Leeds, Leeds, UK. email@example.comShow MeSH
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Mentions: The most studied peptide of insect MAGs is the sex peptide (SP) of D. melanogaster. This 36 amino acid peptide has not been found outside of a sub-group of closely related Drosophilidae. It has multiple signaling roles in the post-mated female, the best known of which is a decrease in sexual receptivity to courting males. Recently, it has been shown that SP and insect myoinhibitory peptides (MIPs) are ligands for the same G-protein coupled receptor despite lack of structural similarity; MIPs, like Aea-HP-1, are relatively short peptides (generally 9–12 amino acids) with an amidated C-terminus. This promiscuity of the SP/MIP receptor led us to test whether Aea-HP-1 might be an additional agonist for this receptor. We therefore carried out experiments to see if Aea-HP-1 could elicit a post-mating response in virgin female D. melanogaster (Fig. 6) . We also tested directly whether Aea-HP-1 was an agonist of the SP/MIP receptor of either D. melanogaster or A. aegypti using an established cell-based assay for receptor activation (Fig. 7) . Aea-HP-1 did not elicit rejection of male advances when injected into the hemocoel of virgin D. melanogaster females and did not activate the SP/MIP receptors up to 10 μM.
Affiliation: Faculty of Biological Sciences, University of Leeds, Leeds, UK. firstname.lastname@example.org