The host-seeking inhibitory peptide, Aea-HP-1, is made in the male accessory gland and transferred to the female during copulation.
Bottom Line: The structure of the peptide with its blocked N- and C-termini confers resistance to metabolic inactivation by MAG peptidases; however the peptide persists for less than 2h in the female reproductive tract after copulation.Aea-HP-1 is not a ligand for the mosquito sex peptide/myoinhibitory peptide receptor.A. aegypti often mate close to the host and therefore it is possible that male-derived Aea-HP-1 induces short-term changes to female host-seeking behavior to reduce potentially lethal encounters with hosts soon after insemination.
Affiliation: Faculty of Biological Sciences, University of Leeds, Leeds, UK. firstname.lastname@example.orgShow MeSH
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Mentions: Confocal microscopy of whole mounted MAGs/SVs stained using the same antibody revealed differential distribution of the immunoreactivity with staining largely restricted to the MAGs and much of this being concentrated in the lumen of the anterior region of the gland (Fig. 4). During the course of this investigation it was noticed that dissected MAGs with SVs attached often released gland contents from the SVs by spontaneous contractions of the MAG muscle layer. It was therefore possible to collect MAG secretions into PBS using a pipette and place this material into acidified methanol. Both Aea-HP-1 and Aea-HP-3 were detected in these secretions by MALDI/TOF-MS (m/z, 1227.8 and 1211.8, respectively), confirming the presence of these peptides as components of the MAG secretions and therefore seminal fluid.
Affiliation: Faculty of Biological Sciences, University of Leeds, Leeds, UK. email@example.com