The host-seeking inhibitory peptide, Aea-HP-1, is made in the male accessory gland and transferred to the female during copulation.
Bottom Line: The structure of the peptide with its blocked N- and C-termini confers resistance to metabolic inactivation by MAG peptidases; however the peptide persists for less than 2h in the female reproductive tract after copulation.Aea-HP-1 is not a ligand for the mosquito sex peptide/myoinhibitory peptide receptor.A. aegypti often mate close to the host and therefore it is possible that male-derived Aea-HP-1 induces short-term changes to female host-seeking behavior to reduce potentially lethal encounters with hosts soon after insemination.
Affiliation: Faculty of Biological Sciences, University of Leeds, Leeds, UK. email@example.comShow MeSH
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Mentions: Aea-HP-1 cross-reacts with antibodies raised to the invertebrate peptide, FMRFamide, presumably because of the common RFamide epitope [4,30,39]. We therefore used a commercially available FMRFamide antibody in an ELISA to monitor HPLC fractions for Aea-HP-1 and any other FMRFamide-like peptides that might be present in the MAGs/SVs extract (Fig. 3). A single peak of ELISA-positive material was eluted from the HPLC column in three consecutive fractions that were also positive for mass ion m/z of 1227.7. All other UV-absorbing fractions gave negative ELISA results, suggesting that Aea-HP-1 was the major peptide component of the extract displaying cross-reactivity to the FMRFamide antibody.
Affiliation: Faculty of Biological Sciences, University of Leeds, Leeds, UK. firstname.lastname@example.org