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The host-seeking inhibitory peptide, Aea-HP-1, is made in the male accessory gland and transferred to the female during copulation.

Naccarati C, Audsley N, Keen JN, Kim JH, Howell GJ, Kim YJ, Isaac RE - Peptides (2011)

Bottom Line: The structure of the peptide with its blocked N- and C-termini confers resistance to metabolic inactivation by MAG peptidases; however the peptide persists for less than 2h in the female reproductive tract after copulation.Aea-HP-1 is not a ligand for the mosquito sex peptide/myoinhibitory peptide receptor.A. aegypti often mate close to the host and therefore it is possible that male-derived Aea-HP-1 induces short-term changes to female host-seeking behavior to reduce potentially lethal encounters with hosts soon after insemination.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Biological Sciences, University of Leeds, Leeds, UK. bs09cn@leeds.ac.uk

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Co-chromatography of FMRFamide immunoreactivity with synthetic Aea-HP-1. An acid–methanol extract of MAGs was fractionated using RP-HPLC. The elution of Aea-HP-1 was monitored using both MALDI/TOF-MS and ELISA for FMRF-amide-like peptides. Aea-HP-1 was detected by MALDI/TOF-MS (molecular ion, m/z 1227.6) in fractions 22–24 which corresponded to the retention time of synthetic Aea-HP-1. The same fractions were the only ones that gave an above background response in the ELISA. The calibration plot for Aea-HP-1 was linear between 10 and 1000 fmol.
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fig0015: Co-chromatography of FMRFamide immunoreactivity with synthetic Aea-HP-1. An acid–methanol extract of MAGs was fractionated using RP-HPLC. The elution of Aea-HP-1 was monitored using both MALDI/TOF-MS and ELISA for FMRF-amide-like peptides. Aea-HP-1 was detected by MALDI/TOF-MS (molecular ion, m/z 1227.6) in fractions 22–24 which corresponded to the retention time of synthetic Aea-HP-1. The same fractions were the only ones that gave an above background response in the ELISA. The calibration plot for Aea-HP-1 was linear between 10 and 1000 fmol.

Mentions: Aea-HP-1 cross-reacts with antibodies raised to the invertebrate peptide, FMRFamide, presumably because of the common RFamide epitope [4,30,39]. We therefore used a commercially available FMRFamide antibody in an ELISA to monitor HPLC fractions for Aea-HP-1 and any other FMRFamide-like peptides that might be present in the MAGs/SVs extract (Fig. 3). A single peak of ELISA-positive material was eluted from the HPLC column in three consecutive fractions that were also positive for mass ion m/z of 1227.7. All other UV-absorbing fractions gave negative ELISA results, suggesting that Aea-HP-1 was the major peptide component of the extract displaying cross-reactivity to the FMRFamide antibody.


The host-seeking inhibitory peptide, Aea-HP-1, is made in the male accessory gland and transferred to the female during copulation.

Naccarati C, Audsley N, Keen JN, Kim JH, Howell GJ, Kim YJ, Isaac RE - Peptides (2011)

Co-chromatography of FMRFamide immunoreactivity with synthetic Aea-HP-1. An acid–methanol extract of MAGs was fractionated using RP-HPLC. The elution of Aea-HP-1 was monitored using both MALDI/TOF-MS and ELISA for FMRF-amide-like peptides. Aea-HP-1 was detected by MALDI/TOF-MS (molecular ion, m/z 1227.6) in fractions 22–24 which corresponded to the retention time of synthetic Aea-HP-1. The same fractions were the only ones that gave an above background response in the ELISA. The calibration plot for Aea-HP-1 was linear between 10 and 1000 fmol.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3368272&req=5

fig0015: Co-chromatography of FMRFamide immunoreactivity with synthetic Aea-HP-1. An acid–methanol extract of MAGs was fractionated using RP-HPLC. The elution of Aea-HP-1 was monitored using both MALDI/TOF-MS and ELISA for FMRF-amide-like peptides. Aea-HP-1 was detected by MALDI/TOF-MS (molecular ion, m/z 1227.6) in fractions 22–24 which corresponded to the retention time of synthetic Aea-HP-1. The same fractions were the only ones that gave an above background response in the ELISA. The calibration plot for Aea-HP-1 was linear between 10 and 1000 fmol.
Mentions: Aea-HP-1 cross-reacts with antibodies raised to the invertebrate peptide, FMRFamide, presumably because of the common RFamide epitope [4,30,39]. We therefore used a commercially available FMRFamide antibody in an ELISA to monitor HPLC fractions for Aea-HP-1 and any other FMRFamide-like peptides that might be present in the MAGs/SVs extract (Fig. 3). A single peak of ELISA-positive material was eluted from the HPLC column in three consecutive fractions that were also positive for mass ion m/z of 1227.7. All other UV-absorbing fractions gave negative ELISA results, suggesting that Aea-HP-1 was the major peptide component of the extract displaying cross-reactivity to the FMRFamide antibody.

Bottom Line: The structure of the peptide with its blocked N- and C-termini confers resistance to metabolic inactivation by MAG peptidases; however the peptide persists for less than 2h in the female reproductive tract after copulation.Aea-HP-1 is not a ligand for the mosquito sex peptide/myoinhibitory peptide receptor.A. aegypti often mate close to the host and therefore it is possible that male-derived Aea-HP-1 induces short-term changes to female host-seeking behavior to reduce potentially lethal encounters with hosts soon after insemination.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Biological Sciences, University of Leeds, Leeds, UK. bs09cn@leeds.ac.uk

Show MeSH
Related in: MedlinePlus