Imaging heart development using high-resolution episcopic microscopy.
Bottom Line: Development of the heart in vertebrate embryos is a complex process in which the organ is continually remodelled as chambers are formed, valves sculpted and connections established to the developing vascular system.A recurrent challenge in this work is how to integrate studies as diverse as those of cardiac gene function and regulation with an appreciation of the localised interactions between cardiac tissues not to mention the manner in which both may be affected by cardiac function itself.Meeting this challenge requires an accurate way to analyse the changes in 3D morphology of the developing heart, which can be swift or protracted and both dramatic or subtle in consequence.
Affiliation: MRC National Institute for Medical Research, London, UK. email@example.comShow MeSH
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Mentions: Similarly, HREM analysis has facilitated quantitative assessment of stenosis or dilation of the great intrathoracic arteries. Coarctation of the aorta or stenosis of the pharyngeal arch arteries and their derivatives often are associated with complex, intra-cardiac and extra-cardiac defects [e.g.] [29,30–33] which can result in prenatal or perinatal lethality. Accurate detection of stenosis in embryonic and foetal blood vessels requires histological sections cut precisely perpendicular to the longitudinal axis of the artery being measured. Technically challenging with adult mice, this conventional approach is impossible with mouse embryos. Its digital equivalent is however straightforward with image volume data — and only HREM data currently provides spatial resolution adequate to yield meaningful measurements [34,35] (Figure 2).
Affiliation: MRC National Institute for Medical Research, London, UK. firstname.lastname@example.org