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Specific expression of Kcna10, Pxn and Odf2 in the organ of Corti.

Carlisle FA, Steel KP, Lewis MA - Gene Expr. Patterns (2012)

Bottom Line: The development of the organ of Corti and the highly specialized cells required for hearing involves a multitude of genes, many of which remain unknown.Kcna10, a tetrameric Shaker-like potassium channel, is expressed strongly in the hair cells themselves.The roles of these genes are yet to be elucidated, but their specific expression patterns imply potential functional significance in the inner ear.

View Article: PubMed Central - PubMed

Affiliation: Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK.

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Related in: MedlinePlus

Immunohistochemistry for Kcna10 expression in the mouse inner ear. Brown indicates positive staining. (A) Cochlear duct at E14.5, showing clear staining lining the interior and exterior of the cochlear duct. (B) Cochlear duct at E18.5, showing moderate staining of the hair cells and faint staining in Kölliker’s organ and stria vascularis. (C) Cochlear duct at P5, showing strong staining of the hair cells, moderate staining in the stria vascularis, spiral prominence and root cells and faint staining in Kölliker’s organ, interdental cells and Reissner’s membrane. (D) Detail of stria vascularis at P5, showing moderate staining. (E) Detail of hair cells at P5, showing strong staining in all hair cells. (F) Crista at P5, showing staining of the hair cells. (G) Organ of Corti at 9 weeks old, showing staining around the cochlear duct, most notably in stria vascularis and hair cells, and in the spiral ganglion. (H) and (I) Crista (H) and macula (I) at 9 weeks old showing strong staining in hair cells, supporting cells and fainter staining in neurons (asterisks). For images A–C, bar indicates 50 μm. For images D–G, bar indicates 20 μm. For images H and I, bar indicates 10 μm. Abbreviations: ko, Kölliker’s organ; sp, spiral prominence; rc, root cells; ic, interdental cells; rm, Reissner’s membrane; sg, spiral ganglion. Neurons are indicated by asterisks, hair cells by arrowheads. Arrows in panel A indicate staining, arrows in B and G indicate the stria vascularis.
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f0015: Immunohistochemistry for Kcna10 expression in the mouse inner ear. Brown indicates positive staining. (A) Cochlear duct at E14.5, showing clear staining lining the interior and exterior of the cochlear duct. (B) Cochlear duct at E18.5, showing moderate staining of the hair cells and faint staining in Kölliker’s organ and stria vascularis. (C) Cochlear duct at P5, showing strong staining of the hair cells, moderate staining in the stria vascularis, spiral prominence and root cells and faint staining in Kölliker’s organ, interdental cells and Reissner’s membrane. (D) Detail of stria vascularis at P5, showing moderate staining. (E) Detail of hair cells at P5, showing strong staining in all hair cells. (F) Crista at P5, showing staining of the hair cells. (G) Organ of Corti at 9 weeks old, showing staining around the cochlear duct, most notably in stria vascularis and hair cells, and in the spiral ganglion. (H) and (I) Crista (H) and macula (I) at 9 weeks old showing strong staining in hair cells, supporting cells and fainter staining in neurons (asterisks). For images A–C, bar indicates 50 μm. For images D–G, bar indicates 20 μm. For images H and I, bar indicates 10 μm. Abbreviations: ko, Kölliker’s organ; sp, spiral prominence; rc, root cells; ic, interdental cells; rm, Reissner’s membrane; sg, spiral ganglion. Neurons are indicated by asterisks, hair cells by arrowheads. Arrows in panel A indicate staining, arrows in B and G indicate the stria vascularis.

Mentions: At E14.5, moderate expression of Kcna10 lined the lumen of the cochlear duct and the basal lining of the duct, as seen in Fig. 3a. Some expression could also be seen in neurons (data not shown); all Kcna10 expression was cytoplasmic. Hair cells in the organ of Corti and the vestibular system began expressing Kcna10 at E16.5 (data not shown). By E18.5, expression was largely limited to the hair cells, stria vascularis, and Kölliker’s organ, although some expression could be seen in other supporting cell types (Fig. 3b). Hair cells showed the highest expression levels. This pattern was continued through P3 (data not shown). At P5, hair cells still showed by far the strongest expression of Kcna10, particularly the apical domains of outer hair cells in the organ of Corti (Fig. 3e). The marginal and intermediate cells in the stria vascularis also showed moderate expression (Fig. 3d), as did the spiral prominence and root cells (Fig. 3c). Furthermore, very faint expression could sometimes be detected in the Reissner’s membrane, interdental cells, and Kölliker’s organ; at this stage all cells lining the cochlear duct appear to express Kcna10 at varying levels. In the vestibular system, hair cells seemed to show heavy expression of Kcna10 at P5, and supporting cells showed mild expression (Fig. 3f). This was consistent with expression in the vestibular system from E16.5 (data not shown). In the adult organ of Corti, Kcna10 is expressed in almost all cells lining the cochlear duct, and also in the spiral ganglion (Fig. 3g), while in the vestibular system hair and supporting cells express Kcna10 and expression is also seen in neurons (Fig. 3h and i).


Specific expression of Kcna10, Pxn and Odf2 in the organ of Corti.

Carlisle FA, Steel KP, Lewis MA - Gene Expr. Patterns (2012)

Immunohistochemistry for Kcna10 expression in the mouse inner ear. Brown indicates positive staining. (A) Cochlear duct at E14.5, showing clear staining lining the interior and exterior of the cochlear duct. (B) Cochlear duct at E18.5, showing moderate staining of the hair cells and faint staining in Kölliker’s organ and stria vascularis. (C) Cochlear duct at P5, showing strong staining of the hair cells, moderate staining in the stria vascularis, spiral prominence and root cells and faint staining in Kölliker’s organ, interdental cells and Reissner’s membrane. (D) Detail of stria vascularis at P5, showing moderate staining. (E) Detail of hair cells at P5, showing strong staining in all hair cells. (F) Crista at P5, showing staining of the hair cells. (G) Organ of Corti at 9 weeks old, showing staining around the cochlear duct, most notably in stria vascularis and hair cells, and in the spiral ganglion. (H) and (I) Crista (H) and macula (I) at 9 weeks old showing strong staining in hair cells, supporting cells and fainter staining in neurons (asterisks). For images A–C, bar indicates 50 μm. For images D–G, bar indicates 20 μm. For images H and I, bar indicates 10 μm. Abbreviations: ko, Kölliker’s organ; sp, spiral prominence; rc, root cells; ic, interdental cells; rm, Reissner’s membrane; sg, spiral ganglion. Neurons are indicated by asterisks, hair cells by arrowheads. Arrows in panel A indicate staining, arrows in B and G indicate the stria vascularis.
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f0015: Immunohistochemistry for Kcna10 expression in the mouse inner ear. Brown indicates positive staining. (A) Cochlear duct at E14.5, showing clear staining lining the interior and exterior of the cochlear duct. (B) Cochlear duct at E18.5, showing moderate staining of the hair cells and faint staining in Kölliker’s organ and stria vascularis. (C) Cochlear duct at P5, showing strong staining of the hair cells, moderate staining in the stria vascularis, spiral prominence and root cells and faint staining in Kölliker’s organ, interdental cells and Reissner’s membrane. (D) Detail of stria vascularis at P5, showing moderate staining. (E) Detail of hair cells at P5, showing strong staining in all hair cells. (F) Crista at P5, showing staining of the hair cells. (G) Organ of Corti at 9 weeks old, showing staining around the cochlear duct, most notably in stria vascularis and hair cells, and in the spiral ganglion. (H) and (I) Crista (H) and macula (I) at 9 weeks old showing strong staining in hair cells, supporting cells and fainter staining in neurons (asterisks). For images A–C, bar indicates 50 μm. For images D–G, bar indicates 20 μm. For images H and I, bar indicates 10 μm. Abbreviations: ko, Kölliker’s organ; sp, spiral prominence; rc, root cells; ic, interdental cells; rm, Reissner’s membrane; sg, spiral ganglion. Neurons are indicated by asterisks, hair cells by arrowheads. Arrows in panel A indicate staining, arrows in B and G indicate the stria vascularis.
Mentions: At E14.5, moderate expression of Kcna10 lined the lumen of the cochlear duct and the basal lining of the duct, as seen in Fig. 3a. Some expression could also be seen in neurons (data not shown); all Kcna10 expression was cytoplasmic. Hair cells in the organ of Corti and the vestibular system began expressing Kcna10 at E16.5 (data not shown). By E18.5, expression was largely limited to the hair cells, stria vascularis, and Kölliker’s organ, although some expression could be seen in other supporting cell types (Fig. 3b). Hair cells showed the highest expression levels. This pattern was continued through P3 (data not shown). At P5, hair cells still showed by far the strongest expression of Kcna10, particularly the apical domains of outer hair cells in the organ of Corti (Fig. 3e). The marginal and intermediate cells in the stria vascularis also showed moderate expression (Fig. 3d), as did the spiral prominence and root cells (Fig. 3c). Furthermore, very faint expression could sometimes be detected in the Reissner’s membrane, interdental cells, and Kölliker’s organ; at this stage all cells lining the cochlear duct appear to express Kcna10 at varying levels. In the vestibular system, hair cells seemed to show heavy expression of Kcna10 at P5, and supporting cells showed mild expression (Fig. 3f). This was consistent with expression in the vestibular system from E16.5 (data not shown). In the adult organ of Corti, Kcna10 is expressed in almost all cells lining the cochlear duct, and also in the spiral ganglion (Fig. 3g), while in the vestibular system hair and supporting cells express Kcna10 and expression is also seen in neurons (Fig. 3h and i).

Bottom Line: The development of the organ of Corti and the highly specialized cells required for hearing involves a multitude of genes, many of which remain unknown.Kcna10, a tetrameric Shaker-like potassium channel, is expressed strongly in the hair cells themselves.The roles of these genes are yet to be elucidated, but their specific expression patterns imply potential functional significance in the inner ear.

View Article: PubMed Central - PubMed

Affiliation: Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK.

Show MeSH
Related in: MedlinePlus