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Specific expression of Kcna10, Pxn and Odf2 in the organ of Corti.

Carlisle FA, Steel KP, Lewis MA - Gene Expr. Patterns (2012)

Bottom Line: The development of the organ of Corti and the highly specialized cells required for hearing involves a multitude of genes, many of which remain unknown.Kcna10, a tetrameric Shaker-like potassium channel, is expressed strongly in the hair cells themselves.The roles of these genes are yet to be elucidated, but their specific expression patterns imply potential functional significance in the inner ear.

View Article: PubMed Central - PubMed

Affiliation: Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK.

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Immunohistochemistry for Odf2 expression in the mouse inner ear. Brown indicates positive staining. (A) Cochlear duct at E14.5, showing clear staining of neurons. (B) Cochlear duct at E16.5, showing faint staining of the hair cells and stronger staining of neural dendrites. (C) Cochlear duct at E18.5, showing faint staining of the hair cells and cells of Claudius, plus marked staining of neural dendrites. (D) Cochlear duct at P5, showing strong staining of interdental cells, root cells and processes, the apical region of Kölliker’s organ, and neural dendrites. (E) Detail of hair cells at P5, showing strong staining of dendrites innervating the hair cells. (F) Macula at P5, showing faint staining of hair cells plus staining of dendrites. (G) Crista at P5, showing strong staining of hair cells and dendrites. (H) Cochlea at 9 weeks old, showing staining in interdental cells, Boettcher cells, root cells and root cell processes. (I) and (J) Crista (I) and macula (J) at 9 weeks old showing staining in vestibular hair cells. For images A–D and H, bar indicates 50 μm. For images E–G, I and J, bar indicates 20 μm. Abbreviations: cc, cells of Claudius; bc, Boettcher cells; ko, Kölliker’s organ; ic, interdental cells. Neurons are indicated by asterisks, hair cells by arrowheads, root cells by an arrow, and root cell processes by double asterisks.
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f0010: Immunohistochemistry for Odf2 expression in the mouse inner ear. Brown indicates positive staining. (A) Cochlear duct at E14.5, showing clear staining of neurons. (B) Cochlear duct at E16.5, showing faint staining of the hair cells and stronger staining of neural dendrites. (C) Cochlear duct at E18.5, showing faint staining of the hair cells and cells of Claudius, plus marked staining of neural dendrites. (D) Cochlear duct at P5, showing strong staining of interdental cells, root cells and processes, the apical region of Kölliker’s organ, and neural dendrites. (E) Detail of hair cells at P5, showing strong staining of dendrites innervating the hair cells. (F) Macula at P5, showing faint staining of hair cells plus staining of dendrites. (G) Crista at P5, showing strong staining of hair cells and dendrites. (H) Cochlea at 9 weeks old, showing staining in interdental cells, Boettcher cells, root cells and root cell processes. (I) and (J) Crista (I) and macula (J) at 9 weeks old showing staining in vestibular hair cells. For images A–D and H, bar indicates 50 μm. For images E–G, I and J, bar indicates 20 μm. Abbreviations: cc, cells of Claudius; bc, Boettcher cells; ko, Kölliker’s organ; ic, interdental cells. Neurons are indicated by asterisks, hair cells by arrowheads, root cells by an arrow, and root cell processes by double asterisks.

Mentions: Expression of Odf2 showed a marked difference between sensory regions in the organ of Corti and in the vestibular system. In the organ of Corti, neural tissue showed clear Odf2 expression right from E14.5 (Fig. 2a). From E14.5 to E18.5, Odf2 expression marked the neural dendrites as they extended to innervate first the inner and then the outer hair cells, as can be followed in Fig. 2a–c. At E18.5 and subsequent ages, the strongest Odf2 expression was in the portions of dendrites directly underneath hair cells, presumably at synapses. Hair cells were faintly stained from E16.5 (Fig. 2b), and the cells of Claudius from E18.5 (Fig. 2c). At P0, root cells showed faint expression as well (data not shown). The root cells and the root cell processes showed strong expression at P3 (data not shown) and P5 (Fig. 2d). At P5, strong expression could also be seen in the apical region of Kölliker’s organ, and moderate expression in the interdental cells (Fig. 2d). The strongest expression was still in the dendrites, though (Fig. 2e). However, at all ages hair cells in the organ of Corti showed only very weak expression of Odf2 (Fig. 2). In contrast, vestibular hair cells in the cristae showed stronger Odf2 expression. This began at E16.5, but was strongest at P5 (Fig. 2g). Hair cells in the maculae did not show anywhere near the same strength of expression, as Fig. 2f demonstrates. As in the cochlea, neurons in the vestibular system showed strong expression of Odf2 (Fig. 2f and g). Like Pxn, Odf2 expression was cytoplasmic. In adult mice, Odf2 expression was marked in the interdental cells, Boettcher cells, the root cells and root cell processes. Fainter expression was also visible in the hair cells of the maculae and cristae (Fig. 2h–j).


Specific expression of Kcna10, Pxn and Odf2 in the organ of Corti.

Carlisle FA, Steel KP, Lewis MA - Gene Expr. Patterns (2012)

Immunohistochemistry for Odf2 expression in the mouse inner ear. Brown indicates positive staining. (A) Cochlear duct at E14.5, showing clear staining of neurons. (B) Cochlear duct at E16.5, showing faint staining of the hair cells and stronger staining of neural dendrites. (C) Cochlear duct at E18.5, showing faint staining of the hair cells and cells of Claudius, plus marked staining of neural dendrites. (D) Cochlear duct at P5, showing strong staining of interdental cells, root cells and processes, the apical region of Kölliker’s organ, and neural dendrites. (E) Detail of hair cells at P5, showing strong staining of dendrites innervating the hair cells. (F) Macula at P5, showing faint staining of hair cells plus staining of dendrites. (G) Crista at P5, showing strong staining of hair cells and dendrites. (H) Cochlea at 9 weeks old, showing staining in interdental cells, Boettcher cells, root cells and root cell processes. (I) and (J) Crista (I) and macula (J) at 9 weeks old showing staining in vestibular hair cells. For images A–D and H, bar indicates 50 μm. For images E–G, I and J, bar indicates 20 μm. Abbreviations: cc, cells of Claudius; bc, Boettcher cells; ko, Kölliker’s organ; ic, interdental cells. Neurons are indicated by asterisks, hair cells by arrowheads, root cells by an arrow, and root cell processes by double asterisks.
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f0010: Immunohistochemistry for Odf2 expression in the mouse inner ear. Brown indicates positive staining. (A) Cochlear duct at E14.5, showing clear staining of neurons. (B) Cochlear duct at E16.5, showing faint staining of the hair cells and stronger staining of neural dendrites. (C) Cochlear duct at E18.5, showing faint staining of the hair cells and cells of Claudius, plus marked staining of neural dendrites. (D) Cochlear duct at P5, showing strong staining of interdental cells, root cells and processes, the apical region of Kölliker’s organ, and neural dendrites. (E) Detail of hair cells at P5, showing strong staining of dendrites innervating the hair cells. (F) Macula at P5, showing faint staining of hair cells plus staining of dendrites. (G) Crista at P5, showing strong staining of hair cells and dendrites. (H) Cochlea at 9 weeks old, showing staining in interdental cells, Boettcher cells, root cells and root cell processes. (I) and (J) Crista (I) and macula (J) at 9 weeks old showing staining in vestibular hair cells. For images A–D and H, bar indicates 50 μm. For images E–G, I and J, bar indicates 20 μm. Abbreviations: cc, cells of Claudius; bc, Boettcher cells; ko, Kölliker’s organ; ic, interdental cells. Neurons are indicated by asterisks, hair cells by arrowheads, root cells by an arrow, and root cell processes by double asterisks.
Mentions: Expression of Odf2 showed a marked difference between sensory regions in the organ of Corti and in the vestibular system. In the organ of Corti, neural tissue showed clear Odf2 expression right from E14.5 (Fig. 2a). From E14.5 to E18.5, Odf2 expression marked the neural dendrites as they extended to innervate first the inner and then the outer hair cells, as can be followed in Fig. 2a–c. At E18.5 and subsequent ages, the strongest Odf2 expression was in the portions of dendrites directly underneath hair cells, presumably at synapses. Hair cells were faintly stained from E16.5 (Fig. 2b), and the cells of Claudius from E18.5 (Fig. 2c). At P0, root cells showed faint expression as well (data not shown). The root cells and the root cell processes showed strong expression at P3 (data not shown) and P5 (Fig. 2d). At P5, strong expression could also be seen in the apical region of Kölliker’s organ, and moderate expression in the interdental cells (Fig. 2d). The strongest expression was still in the dendrites, though (Fig. 2e). However, at all ages hair cells in the organ of Corti showed only very weak expression of Odf2 (Fig. 2). In contrast, vestibular hair cells in the cristae showed stronger Odf2 expression. This began at E16.5, but was strongest at P5 (Fig. 2g). Hair cells in the maculae did not show anywhere near the same strength of expression, as Fig. 2f demonstrates. As in the cochlea, neurons in the vestibular system showed strong expression of Odf2 (Fig. 2f and g). Like Pxn, Odf2 expression was cytoplasmic. In adult mice, Odf2 expression was marked in the interdental cells, Boettcher cells, the root cells and root cell processes. Fainter expression was also visible in the hair cells of the maculae and cristae (Fig. 2h–j).

Bottom Line: The development of the organ of Corti and the highly specialized cells required for hearing involves a multitude of genes, many of which remain unknown.Kcna10, a tetrameric Shaker-like potassium channel, is expressed strongly in the hair cells themselves.The roles of these genes are yet to be elucidated, but their specific expression patterns imply potential functional significance in the inner ear.

View Article: PubMed Central - PubMed

Affiliation: Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK.

Show MeSH
Related in: MedlinePlus