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Specific expression of Kcna10, Pxn and Odf2 in the organ of Corti.

Carlisle FA, Steel KP, Lewis MA - Gene Expr. Patterns (2012)

Bottom Line: The development of the organ of Corti and the highly specialized cells required for hearing involves a multitude of genes, many of which remain unknown.Kcna10, a tetrameric Shaker-like potassium channel, is expressed strongly in the hair cells themselves.The roles of these genes are yet to be elucidated, but their specific expression patterns imply potential functional significance in the inner ear.

View Article: PubMed Central - PubMed

Affiliation: Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK.

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Related in: MedlinePlus

Immunohistochemistry for Paxillin expression in the mouse inner ear. Brown indicates positive staining. (A) Cochlear duct at E14.5, showing discrete staining in non-sensory patches. (B) Cochlear duct at E16.5, showing staining where the scala tympani is opening up and in supporting cells, especially the pillar and Deiters’ cells. (C) Cochlear duct at E18.5, showing staining in the pillar cells, interdental cells, Kölliker’s organ, cells of Claudius, Deiters’ cells, and faintly in the stria vascularis. (D) Cochlear duct at P0, showing staining in the pillar cells, Deiters’ cells, interdental cells, Kölliker’s organ, spiral prominence, Hensen cells, cells of Claudius, a single layer of the Reissner’s membrane, root cells and hair cells. (E) Detail of the hair cells at P3, showing heavy staining in the pillar cells, Deiters’ cells, Hensen cells and cells of Claudius. (F) Detail of the stria vascularis at P3, showing heavy staining in the marginal cells. (G) Detail of the crista at P5, showing heavy staining of supporting and hair cells. (H) Detail of the stria vascularis at P5, showing moderate staining in the marginal cells. (I) Detail of the vestibular duct lining at P5, showing moderate staining. (J) One crista and the common crus at P5, showing staining in supporting cells of the crista and cells lining the vestibular duct. Discrete cells in the otic capsule also show heavy staining. (K) Cochlear duct at 9 weeks old, showing staining in root cell processes, stria vascularis and spiral ganglion. (L) Crista at 9 weeks old showing staining in hair and supporting cells, and also the dark cells. There is faint expression in the neural dendrites. (M) Macula at 9 weeks old showing staining in hair and supporting cells, and faint expression in neural dendrites. For images A–D and K, bar indicates 50 μm. For images E–I, L and M, bar indicates 20 μm. For image J, bar indicates 200 μm. Abbreviations: dc, Deiters’ cells; ic, interdental cells; ko, Kölliker’s organ; sp, spiral prominence; hc, Hensen cells; cc, cells of Claudius; rc, root cells; rm, Reissner’s membrane; st, scala tympani; oc, otic capsule; sg, spiral ganglion; dk, dark cells. Stria vascularis is indicated by an arrow, hair cells by arrowheads, pillar cells by double arrowheads, neural dendrites by asterisks and root cell processes by double asterisks.
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f0005: Immunohistochemistry for Paxillin expression in the mouse inner ear. Brown indicates positive staining. (A) Cochlear duct at E14.5, showing discrete staining in non-sensory patches. (B) Cochlear duct at E16.5, showing staining where the scala tympani is opening up and in supporting cells, especially the pillar and Deiters’ cells. (C) Cochlear duct at E18.5, showing staining in the pillar cells, interdental cells, Kölliker’s organ, cells of Claudius, Deiters’ cells, and faintly in the stria vascularis. (D) Cochlear duct at P0, showing staining in the pillar cells, Deiters’ cells, interdental cells, Kölliker’s organ, spiral prominence, Hensen cells, cells of Claudius, a single layer of the Reissner’s membrane, root cells and hair cells. (E) Detail of the hair cells at P3, showing heavy staining in the pillar cells, Deiters’ cells, Hensen cells and cells of Claudius. (F) Detail of the stria vascularis at P3, showing heavy staining in the marginal cells. (G) Detail of the crista at P5, showing heavy staining of supporting and hair cells. (H) Detail of the stria vascularis at P5, showing moderate staining in the marginal cells. (I) Detail of the vestibular duct lining at P5, showing moderate staining. (J) One crista and the common crus at P5, showing staining in supporting cells of the crista and cells lining the vestibular duct. Discrete cells in the otic capsule also show heavy staining. (K) Cochlear duct at 9 weeks old, showing staining in root cell processes, stria vascularis and spiral ganglion. (L) Crista at 9 weeks old showing staining in hair and supporting cells, and also the dark cells. There is faint expression in the neural dendrites. (M) Macula at 9 weeks old showing staining in hair and supporting cells, and faint expression in neural dendrites. For images A–D and K, bar indicates 50 μm. For images E–I, L and M, bar indicates 20 μm. For image J, bar indicates 200 μm. Abbreviations: dc, Deiters’ cells; ic, interdental cells; ko, Kölliker’s organ; sp, spiral prominence; hc, Hensen cells; cc, cells of Claudius; rc, root cells; rm, Reissner’s membrane; st, scala tympani; oc, otic capsule; sg, spiral ganglion; dk, dark cells. Stria vascularis is indicated by an arrow, hair cells by arrowheads, pillar cells by double arrowheads, neural dendrites by asterisks and root cell processes by double asterisks.

Mentions: Pxn expression was cytoplasmic at all stages, and was never seen in the nuclei. Staining was visible in the organ of Corti from E14.5, in patches of tissue at the modiolar side of the cochlear duct (Fig. 1a). By E16.5, some expression of Pxn could be seen in most supporting tissues, particularly the Deiters’ cells and pillar cells, as well as below the basilar membrane where the scala tympani was opening up (Fig. 1b), but expression in the organ of Corti became much clearer at E18.5 when some expression also could be seen in all epithelial cells lining the cochlear duct (Fig. 1c). By far the strongest expression at E18.5 was, however, in the pillar cells (Fig. 1c). In all of these locations, a gradient of intensity was seen from the basal turn of the cochlea to the apex, with strongest expression at the base. This gradient remained in place through P0, although expression in all locations intensified (Fig. 1d). Expression was also noted in the inner layer of Reissner’s membrane, and to have intensified significantly in the marginal cells at P0. At P3, the general expression pattern was identical to that seen at P0, although expression in the pillar, Deiters’ and marginal cells had increased (Fig. 1e and f), and the root cell processes were showing moderate expression of Pxn (data not shown). However, at P5 expression levels in the marginal cells had decreased (Fig. 1h). All other expression levels and patterns remained the same as that seen at P3 (data not shown). In addition, at P3 and P5 discreet patches of Pxn expression could be seen in the otic capsule, perhaps marking developing osteocytes (Fig. 1j; Vatsa et al., 2008). In the vestibular system, Pxn expression was much simpler: heavy expression was seen in supporting cells, with expression also in the hair cells; expression levels generally increased from E16.5 through P5 (Fig. 1g and j). From E18.5 expression was also noted in cells lining the vestibular ducts, particularly the common crus (Fig. 1i and j). In adult mice, Pxn expression was strongest in the stria vascularis, root cell processes and the spiral ganglion. It was also present in the hair cells of the maculae and cristae, with marked expression in the dark cells adjacent to the crista (Fig. 1k–m).


Specific expression of Kcna10, Pxn and Odf2 in the organ of Corti.

Carlisle FA, Steel KP, Lewis MA - Gene Expr. Patterns (2012)

Immunohistochemistry for Paxillin expression in the mouse inner ear. Brown indicates positive staining. (A) Cochlear duct at E14.5, showing discrete staining in non-sensory patches. (B) Cochlear duct at E16.5, showing staining where the scala tympani is opening up and in supporting cells, especially the pillar and Deiters’ cells. (C) Cochlear duct at E18.5, showing staining in the pillar cells, interdental cells, Kölliker’s organ, cells of Claudius, Deiters’ cells, and faintly in the stria vascularis. (D) Cochlear duct at P0, showing staining in the pillar cells, Deiters’ cells, interdental cells, Kölliker’s organ, spiral prominence, Hensen cells, cells of Claudius, a single layer of the Reissner’s membrane, root cells and hair cells. (E) Detail of the hair cells at P3, showing heavy staining in the pillar cells, Deiters’ cells, Hensen cells and cells of Claudius. (F) Detail of the stria vascularis at P3, showing heavy staining in the marginal cells. (G) Detail of the crista at P5, showing heavy staining of supporting and hair cells. (H) Detail of the stria vascularis at P5, showing moderate staining in the marginal cells. (I) Detail of the vestibular duct lining at P5, showing moderate staining. (J) One crista and the common crus at P5, showing staining in supporting cells of the crista and cells lining the vestibular duct. Discrete cells in the otic capsule also show heavy staining. (K) Cochlear duct at 9 weeks old, showing staining in root cell processes, stria vascularis and spiral ganglion. (L) Crista at 9 weeks old showing staining in hair and supporting cells, and also the dark cells. There is faint expression in the neural dendrites. (M) Macula at 9 weeks old showing staining in hair and supporting cells, and faint expression in neural dendrites. For images A–D and K, bar indicates 50 μm. For images E–I, L and M, bar indicates 20 μm. For image J, bar indicates 200 μm. Abbreviations: dc, Deiters’ cells; ic, interdental cells; ko, Kölliker’s organ; sp, spiral prominence; hc, Hensen cells; cc, cells of Claudius; rc, root cells; rm, Reissner’s membrane; st, scala tympani; oc, otic capsule; sg, spiral ganglion; dk, dark cells. Stria vascularis is indicated by an arrow, hair cells by arrowheads, pillar cells by double arrowheads, neural dendrites by asterisks and root cell processes by double asterisks.
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f0005: Immunohistochemistry for Paxillin expression in the mouse inner ear. Brown indicates positive staining. (A) Cochlear duct at E14.5, showing discrete staining in non-sensory patches. (B) Cochlear duct at E16.5, showing staining where the scala tympani is opening up and in supporting cells, especially the pillar and Deiters’ cells. (C) Cochlear duct at E18.5, showing staining in the pillar cells, interdental cells, Kölliker’s organ, cells of Claudius, Deiters’ cells, and faintly in the stria vascularis. (D) Cochlear duct at P0, showing staining in the pillar cells, Deiters’ cells, interdental cells, Kölliker’s organ, spiral prominence, Hensen cells, cells of Claudius, a single layer of the Reissner’s membrane, root cells and hair cells. (E) Detail of the hair cells at P3, showing heavy staining in the pillar cells, Deiters’ cells, Hensen cells and cells of Claudius. (F) Detail of the stria vascularis at P3, showing heavy staining in the marginal cells. (G) Detail of the crista at P5, showing heavy staining of supporting and hair cells. (H) Detail of the stria vascularis at P5, showing moderate staining in the marginal cells. (I) Detail of the vestibular duct lining at P5, showing moderate staining. (J) One crista and the common crus at P5, showing staining in supporting cells of the crista and cells lining the vestibular duct. Discrete cells in the otic capsule also show heavy staining. (K) Cochlear duct at 9 weeks old, showing staining in root cell processes, stria vascularis and spiral ganglion. (L) Crista at 9 weeks old showing staining in hair and supporting cells, and also the dark cells. There is faint expression in the neural dendrites. (M) Macula at 9 weeks old showing staining in hair and supporting cells, and faint expression in neural dendrites. For images A–D and K, bar indicates 50 μm. For images E–I, L and M, bar indicates 20 μm. For image J, bar indicates 200 μm. Abbreviations: dc, Deiters’ cells; ic, interdental cells; ko, Kölliker’s organ; sp, spiral prominence; hc, Hensen cells; cc, cells of Claudius; rc, root cells; rm, Reissner’s membrane; st, scala tympani; oc, otic capsule; sg, spiral ganglion; dk, dark cells. Stria vascularis is indicated by an arrow, hair cells by arrowheads, pillar cells by double arrowheads, neural dendrites by asterisks and root cell processes by double asterisks.
Mentions: Pxn expression was cytoplasmic at all stages, and was never seen in the nuclei. Staining was visible in the organ of Corti from E14.5, in patches of tissue at the modiolar side of the cochlear duct (Fig. 1a). By E16.5, some expression of Pxn could be seen in most supporting tissues, particularly the Deiters’ cells and pillar cells, as well as below the basilar membrane where the scala tympani was opening up (Fig. 1b), but expression in the organ of Corti became much clearer at E18.5 when some expression also could be seen in all epithelial cells lining the cochlear duct (Fig. 1c). By far the strongest expression at E18.5 was, however, in the pillar cells (Fig. 1c). In all of these locations, a gradient of intensity was seen from the basal turn of the cochlea to the apex, with strongest expression at the base. This gradient remained in place through P0, although expression in all locations intensified (Fig. 1d). Expression was also noted in the inner layer of Reissner’s membrane, and to have intensified significantly in the marginal cells at P0. At P3, the general expression pattern was identical to that seen at P0, although expression in the pillar, Deiters’ and marginal cells had increased (Fig. 1e and f), and the root cell processes were showing moderate expression of Pxn (data not shown). However, at P5 expression levels in the marginal cells had decreased (Fig. 1h). All other expression levels and patterns remained the same as that seen at P3 (data not shown). In addition, at P3 and P5 discreet patches of Pxn expression could be seen in the otic capsule, perhaps marking developing osteocytes (Fig. 1j; Vatsa et al., 2008). In the vestibular system, Pxn expression was much simpler: heavy expression was seen in supporting cells, with expression also in the hair cells; expression levels generally increased from E16.5 through P5 (Fig. 1g and j). From E18.5 expression was also noted in cells lining the vestibular ducts, particularly the common crus (Fig. 1i and j). In adult mice, Pxn expression was strongest in the stria vascularis, root cell processes and the spiral ganglion. It was also present in the hair cells of the maculae and cristae, with marked expression in the dark cells adjacent to the crista (Fig. 1k–m).

Bottom Line: The development of the organ of Corti and the highly specialized cells required for hearing involves a multitude of genes, many of which remain unknown.Kcna10, a tetrameric Shaker-like potassium channel, is expressed strongly in the hair cells themselves.The roles of these genes are yet to be elucidated, but their specific expression patterns imply potential functional significance in the inner ear.

View Article: PubMed Central - PubMed

Affiliation: Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK.

Show MeSH
Related in: MedlinePlus