Limits...
The crude extract from puerariae flower exerts antiobesity and antifatty liver effects in high-fat diet-induced obese mice.

Kamiya T, Sameshima-Kamiya M, Nagamine R, Tsubata M, Ikeguchi M, Takagaki K, Shimada T, Aburada M - Evid Based Complement Alternat Med (2012)

Bottom Line: PFE supplementation significantly reduced body weight and white adipose tissue (WAT) weight.Moreover, in the histological analysis, PFE supplementation improved fatty liver.For adipose tissue, the expressions of hormone-sensitive lipase in WAT and uncoupling protein 1 in brown adipose tissue (BAT) were significantly upregulated.

View Article: PubMed Central - PubMed

Affiliation: Research and Development Division, Toyo Shinyaku Co. Ltd., 7-28 Yayoigaoka, Tosu-shi, Saga 841-0005, Japan.

ABSTRACT
Kudzu, a leguminous plant, has long been used in folk medicine. In particular, its flowers are used in Japanese and Chinese folk medicine for treating hangovers. We focused on the flower of Kudzu (Puerariae thomsonii), and we previously reported the antiobesity effect of Puerariae thomsonii flower extract (PFE) in humans. In this study, we conducted an animal study to investigate the effect of PFE on visceral fat and hepatic lipid levels in mice with diet-induced obesity. In addition, we focused on gene expression profiles to investigate the antiobesity mechanism of PFE. Male C57BL/6J mice were fed a high-fat diet (HFD) or an HFD supplemented with 5% PFE for 14 days. PFE supplementation significantly reduced body weight and white adipose tissue (WAT) weight. Moreover, in the histological analysis, PFE supplementation improved fatty liver. Hepatic reverse transcription-polymerase chain reaction revealed that PFE supplementation downregulated acetyl-CoA carboxylase expression. For adipose tissue, the expressions of hormone-sensitive lipase in WAT and uncoupling protein 1 in brown adipose tissue (BAT) were significantly upregulated. These results suggest that PFE exerts antiobesity and antifatty liver effects in high-fat diet-induced obese mice through suppressing lipogenesis in the liver, stimulating lipolysis in WAT, and promoting thermogenesis in BAT.

No MeSH data available.


Related in: MedlinePlus

Histological analysis of the liver (Oil Red-O staining). The liver was extracted 14 days after commencing the treatment. Oil Red-O staining was performed in frozen liver sections to detect the presence of fat. Lipid droplets are stained red with Oil Red-O. The arrowhead shows the lumen of a blood vessel. These images are representative of observations made on 8 mice per group. (Scale bar: 50 μm).
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3368216&req=5

fig1: Histological analysis of the liver (Oil Red-O staining). The liver was extracted 14 days after commencing the treatment. Oil Red-O staining was performed in frozen liver sections to detect the presence of fat. Lipid droplets are stained red with Oil Red-O. The arrowhead shows the lumen of a blood vessel. These images are representative of observations made on 8 mice per group. (Scale bar: 50 μm).

Mentions: Table 4 and Figure 1 show the hepatic histological analysis data on day 14 after the start of the experiment. In the HFD + PFE group, the development of fatty liver was apparently suppressed.


The crude extract from puerariae flower exerts antiobesity and antifatty liver effects in high-fat diet-induced obese mice.

Kamiya T, Sameshima-Kamiya M, Nagamine R, Tsubata M, Ikeguchi M, Takagaki K, Shimada T, Aburada M - Evid Based Complement Alternat Med (2012)

Histological analysis of the liver (Oil Red-O staining). The liver was extracted 14 days after commencing the treatment. Oil Red-O staining was performed in frozen liver sections to detect the presence of fat. Lipid droplets are stained red with Oil Red-O. The arrowhead shows the lumen of a blood vessel. These images are representative of observations made on 8 mice per group. (Scale bar: 50 μm).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3368216&req=5

fig1: Histological analysis of the liver (Oil Red-O staining). The liver was extracted 14 days after commencing the treatment. Oil Red-O staining was performed in frozen liver sections to detect the presence of fat. Lipid droplets are stained red with Oil Red-O. The arrowhead shows the lumen of a blood vessel. These images are representative of observations made on 8 mice per group. (Scale bar: 50 μm).
Mentions: Table 4 and Figure 1 show the hepatic histological analysis data on day 14 after the start of the experiment. In the HFD + PFE group, the development of fatty liver was apparently suppressed.

Bottom Line: PFE supplementation significantly reduced body weight and white adipose tissue (WAT) weight.Moreover, in the histological analysis, PFE supplementation improved fatty liver.For adipose tissue, the expressions of hormone-sensitive lipase in WAT and uncoupling protein 1 in brown adipose tissue (BAT) were significantly upregulated.

View Article: PubMed Central - PubMed

Affiliation: Research and Development Division, Toyo Shinyaku Co. Ltd., 7-28 Yayoigaoka, Tosu-shi, Saga 841-0005, Japan.

ABSTRACT
Kudzu, a leguminous plant, has long been used in folk medicine. In particular, its flowers are used in Japanese and Chinese folk medicine for treating hangovers. We focused on the flower of Kudzu (Puerariae thomsonii), and we previously reported the antiobesity effect of Puerariae thomsonii flower extract (PFE) in humans. In this study, we conducted an animal study to investigate the effect of PFE on visceral fat and hepatic lipid levels in mice with diet-induced obesity. In addition, we focused on gene expression profiles to investigate the antiobesity mechanism of PFE. Male C57BL/6J mice were fed a high-fat diet (HFD) or an HFD supplemented with 5% PFE for 14 days. PFE supplementation significantly reduced body weight and white adipose tissue (WAT) weight. Moreover, in the histological analysis, PFE supplementation improved fatty liver. Hepatic reverse transcription-polymerase chain reaction revealed that PFE supplementation downregulated acetyl-CoA carboxylase expression. For adipose tissue, the expressions of hormone-sensitive lipase in WAT and uncoupling protein 1 in brown adipose tissue (BAT) were significantly upregulated. These results suggest that PFE exerts antiobesity and antifatty liver effects in high-fat diet-induced obese mice through suppressing lipogenesis in the liver, stimulating lipolysis in WAT, and promoting thermogenesis in BAT.

No MeSH data available.


Related in: MedlinePlus