Limits...
Antimicrobial Activity of Essential Oils against Streptococcus mutans and their Antiproliferative Effects.

Galvão LC, Furletti VF, Bersan SM, da Cunha MG, Ruiz AL, de Carvalho JE, Sartoratto A, Rehder VL, Figueira GM, Teixeira Duarte MC, Ikegaki M, de Alencar SM, Rosalen PL - Evid Based Complement Alternat Med (2012)

Bottom Line: EOs were selected according to predetermined criteria and fractionated using dry column; the resulting fractions were assessed by MIC and MBC, selected as active fractions, and evaluated against S. mutans biofilm.Chemical analyses mainly showed the presence of terpenes.The selected active fractions inhibited S. mutans biofilm formation (P < 0.05) did not affect glycolytic pH drop and were inactive against keratinocytes, normal cell line.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Anesthesiology and Therapeutics, Piracicaba Dental School, University of Campinas (UNICAMP), 13414-903 Piracicaba, SP, Brazil.

ABSTRACT
This study aimed to evaluate the activity of essential oils (EOs) against Streptococcus mutans biofilm by chemically characterizing their fractions responsible for biological and antiproliferative activity. Twenty EO were obtained by hydrodistillation and submitted to the antimicrobial assay (minimum inhibitory (MIC) and bactericidal (MBC) concentrations) against S. mutans UA159. Thin-layer chromatography and gas chromatography/mass spectrometry were used for phytochemical analyses. EOs were selected according to predetermined criteria and fractionated using dry column; the resulting fractions were assessed by MIC and MBC, selected as active fractions, and evaluated against S. mutans biofilm. Biofilms formed were examined using scanning electron microscopy. Selected EOs and their selected active fractions were evaluated for their antiproliferative activity against keratinocytes and seven human tumor cell lines. MIC and MBC values obtained for EO and their active fractions showed strong antimicrobial activity. Chemical analyses mainly showed the presence of terpenes. The selected active fractions inhibited S. mutans biofilm formation (P < 0.05) did not affect glycolytic pH drop and were inactive against keratinocytes, normal cell line. In conclusion, EO showed activity at low concentrations, and their selected active fractions were also effective against biofilm formed by S. mutans and human tumor cell lines.

No MeSH data available.


Related in: MedlinePlus

Influence of selected active fractions Ag4, Bd2, Cs4, and Ls3 from selected essential oils at different concentrations against Streptococcus mutans biofilm formation. All fractions tested were significantly different from the vehicle at all concentrations tested. Kruskal-Wallis test (P < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3368214&req=5

fig1: Influence of selected active fractions Ag4, Bd2, Cs4, and Ls3 from selected essential oils at different concentrations against Streptococcus mutans biofilm formation. All fractions tested were significantly different from the vehicle at all concentrations tested. Kruskal-Wallis test (P < 0.05).

Mentions: Figure 1 shows the development of S. mutans biofilm inhibiton after treatment with selected active fractions. Their growth was measured by optic density at 575 nm. The result showed that the selected active fractions tested at different concentrations were significantly different (P < 0.05) from the vehicle. Moreover, Cs4 and Bd2 fractions presented a better performance since they inhibited more than 90% of biofilm formation at lower concentrations (31.2 μg/mL).


Antimicrobial Activity of Essential Oils against Streptococcus mutans and their Antiproliferative Effects.

Galvão LC, Furletti VF, Bersan SM, da Cunha MG, Ruiz AL, de Carvalho JE, Sartoratto A, Rehder VL, Figueira GM, Teixeira Duarte MC, Ikegaki M, de Alencar SM, Rosalen PL - Evid Based Complement Alternat Med (2012)

Influence of selected active fractions Ag4, Bd2, Cs4, and Ls3 from selected essential oils at different concentrations against Streptococcus mutans biofilm formation. All fractions tested were significantly different from the vehicle at all concentrations tested. Kruskal-Wallis test (P < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3368214&req=5

fig1: Influence of selected active fractions Ag4, Bd2, Cs4, and Ls3 from selected essential oils at different concentrations against Streptococcus mutans biofilm formation. All fractions tested were significantly different from the vehicle at all concentrations tested. Kruskal-Wallis test (P < 0.05).
Mentions: Figure 1 shows the development of S. mutans biofilm inhibiton after treatment with selected active fractions. Their growth was measured by optic density at 575 nm. The result showed that the selected active fractions tested at different concentrations were significantly different (P < 0.05) from the vehicle. Moreover, Cs4 and Bd2 fractions presented a better performance since they inhibited more than 90% of biofilm formation at lower concentrations (31.2 μg/mL).

Bottom Line: EOs were selected according to predetermined criteria and fractionated using dry column; the resulting fractions were assessed by MIC and MBC, selected as active fractions, and evaluated against S. mutans biofilm.Chemical analyses mainly showed the presence of terpenes.The selected active fractions inhibited S. mutans biofilm formation (P < 0.05) did not affect glycolytic pH drop and were inactive against keratinocytes, normal cell line.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Anesthesiology and Therapeutics, Piracicaba Dental School, University of Campinas (UNICAMP), 13414-903 Piracicaba, SP, Brazil.

ABSTRACT
This study aimed to evaluate the activity of essential oils (EOs) against Streptococcus mutans biofilm by chemically characterizing their fractions responsible for biological and antiproliferative activity. Twenty EO were obtained by hydrodistillation and submitted to the antimicrobial assay (minimum inhibitory (MIC) and bactericidal (MBC) concentrations) against S. mutans UA159. Thin-layer chromatography and gas chromatography/mass spectrometry were used for phytochemical analyses. EOs were selected according to predetermined criteria and fractionated using dry column; the resulting fractions were assessed by MIC and MBC, selected as active fractions, and evaluated against S. mutans biofilm. Biofilms formed were examined using scanning electron microscopy. Selected EOs and their selected active fractions were evaluated for their antiproliferative activity against keratinocytes and seven human tumor cell lines. MIC and MBC values obtained for EO and their active fractions showed strong antimicrobial activity. Chemical analyses mainly showed the presence of terpenes. The selected active fractions inhibited S. mutans biofilm formation (P < 0.05) did not affect glycolytic pH drop and were inactive against keratinocytes, normal cell line. In conclusion, EO showed activity at low concentrations, and their selected active fractions were also effective against biofilm formed by S. mutans and human tumor cell lines.

No MeSH data available.


Related in: MedlinePlus