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Effect of S-PRG Eluate on Biofilm Formation and Enzyme Activity of Oral Bacteria.

Yoneda M, Suzuki N, Masuo Y, Fujimoto A, Iha K, Yamada K, Iwamoto T, Hirofuji T - Int J Dent (2012)

Bottom Line: We examined the effect of an S-PRG eluate on various biologic activities of Streptococcus mutans and Porphyromonas gingivalis.S-PRG eluate was found to suppress streptococcal adherence.These results indicate that S-PRG eluate suppresses streptococcal adherence and inhibits the protease and coaggregation activities of P. gingivalis.

View Article: PubMed Central - PubMed

Affiliation: Center for Oral Diseases, Fukuoka Dental College, 3-2-1 Hakataekimae, Hakata-ku 812-0011, Japan.

ABSTRACT
Recently, the antibacterial activity of a composite resin containing prereacted glass ionomer (S-PRG) filler was revealed. We examined the effect of an S-PRG eluate on various biologic activities of Streptococcus mutans and Porphyromonas gingivalis. Adherence ability of S. mutans was evaluated by microtiter plate assay; protease and gelatinase activities of P. gingivalis were examined by synthetic substrate hydrolysis and gelatin film spot assay, respectively. Coaggregation of P. gingivalis with Fusobacterium nucleatum was also examined. S-PRG eluate was found to suppress streptococcal adherence. S-PRG eluate inhibited the protease and gelatinase activities of P. gingivalis and the coaggregation between P. gingivalis and F. nucleatum. These results indicate that S-PRG eluate suppresses streptococcal adherence and inhibits the protease and coaggregation activities of P. gingivalis. These findings may prompt research into novel strategies for preventing caries and periodontitis.

No MeSH data available.


Related in: MedlinePlus

Coaggregation between P. gingivalis and F. nucleatum in the presence and absence of S-PRG eluate. (a): photographs of coaggregation. (b): results of coaggregation assay.
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fig4: Coaggregation between P. gingivalis and F. nucleatum in the presence and absence of S-PRG eluate. (a): photographs of coaggregation. (b): results of coaggregation assay.

Mentions: F. nucleatum ATCC25585 exhibited coaggregation with P. gingivalis ATCC33277. Conversely, F. nucleatum ATCC10953 did not coaggregate with P. gingivalis ATCC33277. S-PRG inhibited coaggregation, as shown in Figure 4. When 10% of CB was substituted for S-PRG eluate, only weak coaggregation was observed between P. gingivalis and F. nucleatum ATCC25585. When 50% of CB was substituted for S-PRG eluate, no coaggregation was observed between these strains.


Effect of S-PRG Eluate on Biofilm Formation and Enzyme Activity of Oral Bacteria.

Yoneda M, Suzuki N, Masuo Y, Fujimoto A, Iha K, Yamada K, Iwamoto T, Hirofuji T - Int J Dent (2012)

Coaggregation between P. gingivalis and F. nucleatum in the presence and absence of S-PRG eluate. (a): photographs of coaggregation. (b): results of coaggregation assay.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3368200&req=5

fig4: Coaggregation between P. gingivalis and F. nucleatum in the presence and absence of S-PRG eluate. (a): photographs of coaggregation. (b): results of coaggregation assay.
Mentions: F. nucleatum ATCC25585 exhibited coaggregation with P. gingivalis ATCC33277. Conversely, F. nucleatum ATCC10953 did not coaggregate with P. gingivalis ATCC33277. S-PRG inhibited coaggregation, as shown in Figure 4. When 10% of CB was substituted for S-PRG eluate, only weak coaggregation was observed between P. gingivalis and F. nucleatum ATCC25585. When 50% of CB was substituted for S-PRG eluate, no coaggregation was observed between these strains.

Bottom Line: We examined the effect of an S-PRG eluate on various biologic activities of Streptococcus mutans and Porphyromonas gingivalis.S-PRG eluate was found to suppress streptococcal adherence.These results indicate that S-PRG eluate suppresses streptococcal adherence and inhibits the protease and coaggregation activities of P. gingivalis.

View Article: PubMed Central - PubMed

Affiliation: Center for Oral Diseases, Fukuoka Dental College, 3-2-1 Hakataekimae, Hakata-ku 812-0011, Japan.

ABSTRACT
Recently, the antibacterial activity of a composite resin containing prereacted glass ionomer (S-PRG) filler was revealed. We examined the effect of an S-PRG eluate on various biologic activities of Streptococcus mutans and Porphyromonas gingivalis. Adherence ability of S. mutans was evaluated by microtiter plate assay; protease and gelatinase activities of P. gingivalis were examined by synthetic substrate hydrolysis and gelatin film spot assay, respectively. Coaggregation of P. gingivalis with Fusobacterium nucleatum was also examined. S-PRG eluate was found to suppress streptococcal adherence. S-PRG eluate inhibited the protease and gelatinase activities of P. gingivalis and the coaggregation between P. gingivalis and F. nucleatum. These results indicate that S-PRG eluate suppresses streptococcal adherence and inhibits the protease and coaggregation activities of P. gingivalis. These findings may prompt research into novel strategies for preventing caries and periodontitis.

No MeSH data available.


Related in: MedlinePlus