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Effect of S-PRG Eluate on Biofilm Formation and Enzyme Activity of Oral Bacteria.

Yoneda M, Suzuki N, Masuo Y, Fujimoto A, Iha K, Yamada K, Iwamoto T, Hirofuji T - Int J Dent (2012)

Bottom Line: We examined the effect of an S-PRG eluate on various biologic activities of Streptococcus mutans and Porphyromonas gingivalis.S-PRG eluate was found to suppress streptococcal adherence.These results indicate that S-PRG eluate suppresses streptococcal adherence and inhibits the protease and coaggregation activities of P. gingivalis.

View Article: PubMed Central - PubMed

Affiliation: Center for Oral Diseases, Fukuoka Dental College, 3-2-1 Hakataekimae, Hakata-ku 812-0011, Japan.

ABSTRACT
Recently, the antibacterial activity of a composite resin containing prereacted glass ionomer (S-PRG) filler was revealed. We examined the effect of an S-PRG eluate on various biologic activities of Streptococcus mutans and Porphyromonas gingivalis. Adherence ability of S. mutans was evaluated by microtiter plate assay; protease and gelatinase activities of P. gingivalis were examined by synthetic substrate hydrolysis and gelatin film spot assay, respectively. Coaggregation of P. gingivalis with Fusobacterium nucleatum was also examined. S-PRG eluate was found to suppress streptococcal adherence. S-PRG eluate inhibited the protease and gelatinase activities of P. gingivalis and the coaggregation between P. gingivalis and F. nucleatum. These results indicate that S-PRG eluate suppresses streptococcal adherence and inhibits the protease and coaggregation activities of P. gingivalis. These findings may prompt research into novel strategies for preventing caries and periodontitis.

No MeSH data available.


Related in: MedlinePlus

Gelatinase spot assay. Different amounts of S-PRG eluate were added to the reaction mixture. P.-gingivalis-sonicated extract was then added and the mixture was serially diluted with distilled water. A 15 μL spot of each mixture was placed onto gelatin-coated X-ray film and incubated at 37°C for 2 h in a humidified atmosphere before being washed with tap water. Removal of the gelatin surface beneath the spotted area revealed the base color of the film and indicated the presence of gelatinase activity. (a) Photograph of X-ray film after assay. (b) Results of gelatinase spot assay.
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fig3: Gelatinase spot assay. Different amounts of S-PRG eluate were added to the reaction mixture. P.-gingivalis-sonicated extract was then added and the mixture was serially diluted with distilled water. A 15 μL spot of each mixture was placed onto gelatin-coated X-ray film and incubated at 37°C for 2 h in a humidified atmosphere before being washed with tap water. Removal of the gelatin surface beneath the spotted area revealed the base color of the film and indicated the presence of gelatinase activity. (a) Photograph of X-ray film after assay. (b) Results of gelatinase spot assay.

Mentions: Figure 3 shows the result of the gelatinase spot assay. S-PRG inhibited the gelatinase activity of P. gingivalis. This inhibitory effect was observed at a 100-fold dilution of sonicated extract. S-PRG eluate and the buffer did not show any effect on the gelatin film.


Effect of S-PRG Eluate on Biofilm Formation and Enzyme Activity of Oral Bacteria.

Yoneda M, Suzuki N, Masuo Y, Fujimoto A, Iha K, Yamada K, Iwamoto T, Hirofuji T - Int J Dent (2012)

Gelatinase spot assay. Different amounts of S-PRG eluate were added to the reaction mixture. P.-gingivalis-sonicated extract was then added and the mixture was serially diluted with distilled water. A 15 μL spot of each mixture was placed onto gelatin-coated X-ray film and incubated at 37°C for 2 h in a humidified atmosphere before being washed with tap water. Removal of the gelatin surface beneath the spotted area revealed the base color of the film and indicated the presence of gelatinase activity. (a) Photograph of X-ray film after assay. (b) Results of gelatinase spot assay.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3368200&req=5

fig3: Gelatinase spot assay. Different amounts of S-PRG eluate were added to the reaction mixture. P.-gingivalis-sonicated extract was then added and the mixture was serially diluted with distilled water. A 15 μL spot of each mixture was placed onto gelatin-coated X-ray film and incubated at 37°C for 2 h in a humidified atmosphere before being washed with tap water. Removal of the gelatin surface beneath the spotted area revealed the base color of the film and indicated the presence of gelatinase activity. (a) Photograph of X-ray film after assay. (b) Results of gelatinase spot assay.
Mentions: Figure 3 shows the result of the gelatinase spot assay. S-PRG inhibited the gelatinase activity of P. gingivalis. This inhibitory effect was observed at a 100-fold dilution of sonicated extract. S-PRG eluate and the buffer did not show any effect on the gelatin film.

Bottom Line: We examined the effect of an S-PRG eluate on various biologic activities of Streptococcus mutans and Porphyromonas gingivalis.S-PRG eluate was found to suppress streptococcal adherence.These results indicate that S-PRG eluate suppresses streptococcal adherence and inhibits the protease and coaggregation activities of P. gingivalis.

View Article: PubMed Central - PubMed

Affiliation: Center for Oral Diseases, Fukuoka Dental College, 3-2-1 Hakataekimae, Hakata-ku 812-0011, Japan.

ABSTRACT
Recently, the antibacterial activity of a composite resin containing prereacted glass ionomer (S-PRG) filler was revealed. We examined the effect of an S-PRG eluate on various biologic activities of Streptococcus mutans and Porphyromonas gingivalis. Adherence ability of S. mutans was evaluated by microtiter plate assay; protease and gelatinase activities of P. gingivalis were examined by synthetic substrate hydrolysis and gelatin film spot assay, respectively. Coaggregation of P. gingivalis with Fusobacterium nucleatum was also examined. S-PRG eluate was found to suppress streptococcal adherence. S-PRG eluate inhibited the protease and gelatinase activities of P. gingivalis and the coaggregation between P. gingivalis and F. nucleatum. These results indicate that S-PRG eluate suppresses streptococcal adherence and inhibits the protease and coaggregation activities of P. gingivalis. These findings may prompt research into novel strategies for preventing caries and periodontitis.

No MeSH data available.


Related in: MedlinePlus