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Effect of S-PRG Eluate on Biofilm Formation and Enzyme Activity of Oral Bacteria.

Yoneda M, Suzuki N, Masuo Y, Fujimoto A, Iha K, Yamada K, Iwamoto T, Hirofuji T - Int J Dent (2012)

Bottom Line: We examined the effect of an S-PRG eluate on various biologic activities of Streptococcus mutans and Porphyromonas gingivalis.S-PRG eluate was found to suppress streptococcal adherence.These results indicate that S-PRG eluate suppresses streptococcal adherence and inhibits the protease and coaggregation activities of P. gingivalis.

View Article: PubMed Central - PubMed

Affiliation: Center for Oral Diseases, Fukuoka Dental College, 3-2-1 Hakataekimae, Hakata-ku 812-0011, Japan.

ABSTRACT
Recently, the antibacterial activity of a composite resin containing prereacted glass ionomer (S-PRG) filler was revealed. We examined the effect of an S-PRG eluate on various biologic activities of Streptococcus mutans and Porphyromonas gingivalis. Adherence ability of S. mutans was evaluated by microtiter plate assay; protease and gelatinase activities of P. gingivalis were examined by synthetic substrate hydrolysis and gelatin film spot assay, respectively. Coaggregation of P. gingivalis with Fusobacterium nucleatum was also examined. S-PRG eluate was found to suppress streptococcal adherence. S-PRG eluate inhibited the protease and gelatinase activities of P. gingivalis and the coaggregation between P. gingivalis and F. nucleatum. These results indicate that S-PRG eluate suppresses streptococcal adherence and inhibits the protease and coaggregation activities of P. gingivalis. These findings may prompt research into novel strategies for preventing caries and periodontitis.

No MeSH data available.


Related in: MedlinePlus

BAPNA hydrolysis activity (as an indicator of protease activity) in the presence and absence of S-PRG eluate. P.-gingivalis-sonicated extract was added to the reaction mixture. To analyze the effect of S-PRG, distilled water in control mixture (triangle) was replaced with S-PRG solution (circle). Colored metabolites were quantified using a colorimetric absorbance assay at 405 nm. Data are representative of three independent experiments and the bars indicate the standard deviation.
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fig2: BAPNA hydrolysis activity (as an indicator of protease activity) in the presence and absence of S-PRG eluate. P.-gingivalis-sonicated extract was added to the reaction mixture. To analyze the effect of S-PRG, distilled water in control mixture (triangle) was replaced with S-PRG solution (circle). Colored metabolites were quantified using a colorimetric absorbance assay at 405 nm. Data are representative of three independent experiments and the bars indicate the standard deviation.

Mentions: A sonicated extract sample, prepared from 50 μg/mL P. gingivalis cells, was used. S-PRG showed a 20% inhibitory effect on protease activity (Figure 2). However, no effect of S-PRG was observed when the amount of P.-gingivalis-sonicated extract was increased or decreased.


Effect of S-PRG Eluate on Biofilm Formation and Enzyme Activity of Oral Bacteria.

Yoneda M, Suzuki N, Masuo Y, Fujimoto A, Iha K, Yamada K, Iwamoto T, Hirofuji T - Int J Dent (2012)

BAPNA hydrolysis activity (as an indicator of protease activity) in the presence and absence of S-PRG eluate. P.-gingivalis-sonicated extract was added to the reaction mixture. To analyze the effect of S-PRG, distilled water in control mixture (triangle) was replaced with S-PRG solution (circle). Colored metabolites were quantified using a colorimetric absorbance assay at 405 nm. Data are representative of three independent experiments and the bars indicate the standard deviation.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3368200&req=5

fig2: BAPNA hydrolysis activity (as an indicator of protease activity) in the presence and absence of S-PRG eluate. P.-gingivalis-sonicated extract was added to the reaction mixture. To analyze the effect of S-PRG, distilled water in control mixture (triangle) was replaced with S-PRG solution (circle). Colored metabolites were quantified using a colorimetric absorbance assay at 405 nm. Data are representative of three independent experiments and the bars indicate the standard deviation.
Mentions: A sonicated extract sample, prepared from 50 μg/mL P. gingivalis cells, was used. S-PRG showed a 20% inhibitory effect on protease activity (Figure 2). However, no effect of S-PRG was observed when the amount of P.-gingivalis-sonicated extract was increased or decreased.

Bottom Line: We examined the effect of an S-PRG eluate on various biologic activities of Streptococcus mutans and Porphyromonas gingivalis.S-PRG eluate was found to suppress streptococcal adherence.These results indicate that S-PRG eluate suppresses streptococcal adherence and inhibits the protease and coaggregation activities of P. gingivalis.

View Article: PubMed Central - PubMed

Affiliation: Center for Oral Diseases, Fukuoka Dental College, 3-2-1 Hakataekimae, Hakata-ku 812-0011, Japan.

ABSTRACT
Recently, the antibacterial activity of a composite resin containing prereacted glass ionomer (S-PRG) filler was revealed. We examined the effect of an S-PRG eluate on various biologic activities of Streptococcus mutans and Porphyromonas gingivalis. Adherence ability of S. mutans was evaluated by microtiter plate assay; protease and gelatinase activities of P. gingivalis were examined by synthetic substrate hydrolysis and gelatin film spot assay, respectively. Coaggregation of P. gingivalis with Fusobacterium nucleatum was also examined. S-PRG eluate was found to suppress streptococcal adherence. S-PRG eluate inhibited the protease and gelatinase activities of P. gingivalis and the coaggregation between P. gingivalis and F. nucleatum. These results indicate that S-PRG eluate suppresses streptococcal adherence and inhibits the protease and coaggregation activities of P. gingivalis. These findings may prompt research into novel strategies for preventing caries and periodontitis.

No MeSH data available.


Related in: MedlinePlus