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Effect of S-PRG Eluate on Biofilm Formation and Enzyme Activity of Oral Bacteria.

Yoneda M, Suzuki N, Masuo Y, Fujimoto A, Iha K, Yamada K, Iwamoto T, Hirofuji T - Int J Dent (2012)

Bottom Line: We examined the effect of an S-PRG eluate on various biologic activities of Streptococcus mutans and Porphyromonas gingivalis.S-PRG eluate was found to suppress streptococcal adherence.These results indicate that S-PRG eluate suppresses streptococcal adherence and inhibits the protease and coaggregation activities of P. gingivalis.

View Article: PubMed Central - PubMed

Affiliation: Center for Oral Diseases, Fukuoka Dental College, 3-2-1 Hakataekimae, Hakata-ku 812-0011, Japan.

ABSTRACT
Recently, the antibacterial activity of a composite resin containing prereacted glass ionomer (S-PRG) filler was revealed. We examined the effect of an S-PRG eluate on various biologic activities of Streptococcus mutans and Porphyromonas gingivalis. Adherence ability of S. mutans was evaluated by microtiter plate assay; protease and gelatinase activities of P. gingivalis were examined by synthetic substrate hydrolysis and gelatin film spot assay, respectively. Coaggregation of P. gingivalis with Fusobacterium nucleatum was also examined. S-PRG eluate was found to suppress streptococcal adherence. S-PRG eluate inhibited the protease and gelatinase activities of P. gingivalis and the coaggregation between P. gingivalis and F. nucleatum. These results indicate that S-PRG eluate suppresses streptococcal adherence and inhibits the protease and coaggregation activities of P. gingivalis. These findings may prompt research into novel strategies for preventing caries and periodontitis.

No MeSH data available.


Related in: MedlinePlus

Adherence of Streptococcus mutans JCM 5705 in the presence and absence of PBS or S-PRG eluate. To evaluate the effect of S-PRG eluate on adherence, different percentages of S-PRG were substituted for culture medium (circle). For the control, phosphate-buffered saline was used instead of S-PRG (triangle). The plates were incubated at 37°C for 16 h under anaerobic conditions, stained with 0.25% safranin, and the amount of stain in an ethanol-solubilized sample quantified by measuring absorbance at 492 nm. Data are representative of three independent experiments and the bars indicate the standard deviation.
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fig1: Adherence of Streptococcus mutans JCM 5705 in the presence and absence of PBS or S-PRG eluate. To evaluate the effect of S-PRG eluate on adherence, different percentages of S-PRG were substituted for culture medium (circle). For the control, phosphate-buffered saline was used instead of S-PRG (triangle). The plates were incubated at 37°C for 16 h under anaerobic conditions, stained with 0.25% safranin, and the amount of stain in an ethanol-solubilized sample quantified by measuring absorbance at 492 nm. Data are representative of three independent experiments and the bars indicate the standard deviation.

Mentions: Substitution of culture medium with either PBS or S-PRG reduced adherence by S. mutans. S-PRG showed the greatest suppressive effect (Figure 1). When more than 20% of culture medium was substituted for S-PRG eluate, approximately 20% less bacteria were adhered.


Effect of S-PRG Eluate on Biofilm Formation and Enzyme Activity of Oral Bacteria.

Yoneda M, Suzuki N, Masuo Y, Fujimoto A, Iha K, Yamada K, Iwamoto T, Hirofuji T - Int J Dent (2012)

Adherence of Streptococcus mutans JCM 5705 in the presence and absence of PBS or S-PRG eluate. To evaluate the effect of S-PRG eluate on adherence, different percentages of S-PRG were substituted for culture medium (circle). For the control, phosphate-buffered saline was used instead of S-PRG (triangle). The plates were incubated at 37°C for 16 h under anaerobic conditions, stained with 0.25% safranin, and the amount of stain in an ethanol-solubilized sample quantified by measuring absorbance at 492 nm. Data are representative of three independent experiments and the bars indicate the standard deviation.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3368200&req=5

fig1: Adherence of Streptococcus mutans JCM 5705 in the presence and absence of PBS or S-PRG eluate. To evaluate the effect of S-PRG eluate on adherence, different percentages of S-PRG were substituted for culture medium (circle). For the control, phosphate-buffered saline was used instead of S-PRG (triangle). The plates were incubated at 37°C for 16 h under anaerobic conditions, stained with 0.25% safranin, and the amount of stain in an ethanol-solubilized sample quantified by measuring absorbance at 492 nm. Data are representative of three independent experiments and the bars indicate the standard deviation.
Mentions: Substitution of culture medium with either PBS or S-PRG reduced adherence by S. mutans. S-PRG showed the greatest suppressive effect (Figure 1). When more than 20% of culture medium was substituted for S-PRG eluate, approximately 20% less bacteria were adhered.

Bottom Line: We examined the effect of an S-PRG eluate on various biologic activities of Streptococcus mutans and Porphyromonas gingivalis.S-PRG eluate was found to suppress streptococcal adherence.These results indicate that S-PRG eluate suppresses streptococcal adherence and inhibits the protease and coaggregation activities of P. gingivalis.

View Article: PubMed Central - PubMed

Affiliation: Center for Oral Diseases, Fukuoka Dental College, 3-2-1 Hakataekimae, Hakata-ku 812-0011, Japan.

ABSTRACT
Recently, the antibacterial activity of a composite resin containing prereacted glass ionomer (S-PRG) filler was revealed. We examined the effect of an S-PRG eluate on various biologic activities of Streptococcus mutans and Porphyromonas gingivalis. Adherence ability of S. mutans was evaluated by microtiter plate assay; protease and gelatinase activities of P. gingivalis were examined by synthetic substrate hydrolysis and gelatin film spot assay, respectively. Coaggregation of P. gingivalis with Fusobacterium nucleatum was also examined. S-PRG eluate was found to suppress streptococcal adherence. S-PRG eluate inhibited the protease and gelatinase activities of P. gingivalis and the coaggregation between P. gingivalis and F. nucleatum. These results indicate that S-PRG eluate suppresses streptococcal adherence and inhibits the protease and coaggregation activities of P. gingivalis. These findings may prompt research into novel strategies for preventing caries and periodontitis.

No MeSH data available.


Related in: MedlinePlus