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Rapid quantification of bacteria in infected root canals using fluorescence reagents and a membrane filter: a pilot study on its clinical application to the evaluation of the outcomes of endodontic treatment.

Sato T, Yamaki K, Ishida N, Shoji M, Sato E, Abiko Y, Hashimoto K, Takeuchi Y, Matsuyama J, Shimauchi H, Takahashi N - Int J Dent (2012)

Bottom Line: Then, the bacterial count (CFU) was measured using fluorescence reagents (4',6'-diamidino-2-phenylindole and propidium iodide) and the polycarbonate membrane filter by Bioplorer.Results.In the present pilot study, rapid detection and quantification of bacteria in infected root canals were found to be successfully performed using fluorescence reagents and a membrane filter (Bioplorer analysis).

View Article: PubMed Central - PubMed

Affiliation: Division of Oral Ecology and Biochemistry, Tohoku University Graduate School of Dentistry, Sendai 980-8575, Japan.

ABSTRACT
Objective. The bacterial examination has been performed during the course of the root canal treatment. In the present pilot study, the new developed method, using fluorescence reagents and a membrane filter, was applied to the detection and quantification of bacteria in infected root canals, in order to evaluate the outcomes of the treatment. Methods. Six infected root canals with periapical lesions from 5 subjects were included. Informed consent was obtained from all subjects (age ranges, 23-79 years). Samples from infected root canals were collected at the beginning of the treatment (termed #25 First), the end of the first day of treatment (termed #55 First), and the next appointment day (termed #55 Second). Then, the bacterial count (CFU) was measured using fluorescence reagents (4',6'-diamidino-2-phenylindole and propidium iodide) and the polycarbonate membrane filter by Bioplorer. Results. The mean ± SD of CFU in the sample of "#25 First" was (1.0 ± 1.4) × 10(5). As the root canal treatment progressed, the CFU decreased as 7.9 × 10(3) (#55 First) and 4.3 × 10(2) (#55 Second). Conclusion. In the present pilot study, rapid detection and quantification of bacteria in infected root canals were found to be successfully performed using fluorescence reagents and a membrane filter (Bioplorer analysis).

No MeSH data available.


Related in: MedlinePlus

The mean bacterial counts (CFUs) in the samples (cases 1 to 6) at #25 First (at the beginning of the treatment), #55 First (at the end of the first day of treatment), and #55 Second (at the next appointment day) by Bioplorer and culture.
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Related In: Results  -  Collection


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fig1: The mean bacterial counts (CFUs) in the samples (cases 1 to 6) at #25 First (at the beginning of the treatment), #55 First (at the end of the first day of treatment), and #55 Second (at the next appointment day) by Bioplorer and culture.

Mentions: The mean ± SD bacterial counts (CFU) in the sample of “#25 First” were (1.0 ± 1.4) × 105 and (0.83 ± 1.6) × 104 by Bioplorer and culture, respectively (Figure 1). As the root canal treatment progressed, the CFU decreased as 7.9 × 103 (#55 First) and 4.3 × 102 (#55 Second) by Bioplorer analysis. Similarly, the CFU in the sample of “#55 First” and “#55 Second” analyzed by culture decreased compared with “#25 First,” and in particular, no bacteria were virtually detected in the sample of “#55 Second” by culture (Figure 1).


Rapid quantification of bacteria in infected root canals using fluorescence reagents and a membrane filter: a pilot study on its clinical application to the evaluation of the outcomes of endodontic treatment.

Sato T, Yamaki K, Ishida N, Shoji M, Sato E, Abiko Y, Hashimoto K, Takeuchi Y, Matsuyama J, Shimauchi H, Takahashi N - Int J Dent (2012)

The mean bacterial counts (CFUs) in the samples (cases 1 to 6) at #25 First (at the beginning of the treatment), #55 First (at the end of the first day of treatment), and #55 Second (at the next appointment day) by Bioplorer and culture.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3368189&req=5

fig1: The mean bacterial counts (CFUs) in the samples (cases 1 to 6) at #25 First (at the beginning of the treatment), #55 First (at the end of the first day of treatment), and #55 Second (at the next appointment day) by Bioplorer and culture.
Mentions: The mean ± SD bacterial counts (CFU) in the sample of “#25 First” were (1.0 ± 1.4) × 105 and (0.83 ± 1.6) × 104 by Bioplorer and culture, respectively (Figure 1). As the root canal treatment progressed, the CFU decreased as 7.9 × 103 (#55 First) and 4.3 × 102 (#55 Second) by Bioplorer analysis. Similarly, the CFU in the sample of “#55 First” and “#55 Second” analyzed by culture decreased compared with “#25 First,” and in particular, no bacteria were virtually detected in the sample of “#55 Second” by culture (Figure 1).

Bottom Line: Then, the bacterial count (CFU) was measured using fluorescence reagents (4',6'-diamidino-2-phenylindole and propidium iodide) and the polycarbonate membrane filter by Bioplorer.Results.In the present pilot study, rapid detection and quantification of bacteria in infected root canals were found to be successfully performed using fluorescence reagents and a membrane filter (Bioplorer analysis).

View Article: PubMed Central - PubMed

Affiliation: Division of Oral Ecology and Biochemistry, Tohoku University Graduate School of Dentistry, Sendai 980-8575, Japan.

ABSTRACT
Objective. The bacterial examination has been performed during the course of the root canal treatment. In the present pilot study, the new developed method, using fluorescence reagents and a membrane filter, was applied to the detection and quantification of bacteria in infected root canals, in order to evaluate the outcomes of the treatment. Methods. Six infected root canals with periapical lesions from 5 subjects were included. Informed consent was obtained from all subjects (age ranges, 23-79 years). Samples from infected root canals were collected at the beginning of the treatment (termed #25 First), the end of the first day of treatment (termed #55 First), and the next appointment day (termed #55 Second). Then, the bacterial count (CFU) was measured using fluorescence reagents (4',6'-diamidino-2-phenylindole and propidium iodide) and the polycarbonate membrane filter by Bioplorer. Results. The mean ± SD of CFU in the sample of "#25 First" was (1.0 ± 1.4) × 10(5). As the root canal treatment progressed, the CFU decreased as 7.9 × 10(3) (#55 First) and 4.3 × 10(2) (#55 Second). Conclusion. In the present pilot study, rapid detection and quantification of bacteria in infected root canals were found to be successfully performed using fluorescence reagents and a membrane filter (Bioplorer analysis).

No MeSH data available.


Related in: MedlinePlus