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Passatempo virus, a vaccinia virus strain, Brazil.

Leite JA, Drumond BP, Trindade GS, Lobato ZI, da Fonseca FG, dos SJ, Madureira MC, Guedes MI, Ferreira JM, Bonjardim CA, Ferreira PC, Kroon EG - Emerging Infect. Dis. (2005)

Bottom Line: Passatempo virus was isolated during a zoonotic outbreak.Biologic features and molecular characterization of hemagglutinin, thymidine kinase, and vaccinia growth factor genes suggested a vaccinia virus infection, which strengthens the idea of the reemergence and circulation of vaccinia virus in Brazil.Molecular polymorphisms indicated that Passatempo virus is a different isolate.

View Article: PubMed Central - PubMed

Affiliation: Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.

ABSTRACT
Passatempo virus was isolated during a zoonotic outbreak. Biologic features and molecular characterization of hemagglutinin, thymidine kinase, and vaccinia growth factor genes suggested a vaccinia virus infection, which strengthens the idea of the reemergence and circulation of vaccinia virus in Brazil. Molecular polymorphisms indicated that Passatempo virus is a different isolate.

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Related in: MedlinePlus

Alignment of Passatempo virus (PSTV) hemagluttinin antigen (HA) and vaccinia grown factor (VGF) amino acid inferred sequences with other orthopoxvirus sequences. 1, HA sequence of PSTV (DQ070848) was compared to the region corresponding to amino acids 6 to 309 of vaccinia virus (VACV-WR, AY243312) HA sequence and to HA sequences of Araçatuba virus (ARAV, AY523994); Cantagalo virus (CTGV, AF229247); vaccinia virus IOC (VACV-IOC, AF229248); vaccinia virus Lister (VAVC-LST, AF375124) and cowpox virus Brighton Red (BR) (CPXV-BR, AF482758). 2– The VGF sequence of PSTV (DQ085462) was compared to the region corresponding to amino acids 19 to 135 of vaccinia virus WR (VACV-WR, AY243312) and to the same region of Araçatuba virus (ARAV, AF503170), vaccinia virus Lister (VACV-LST, AY678276); and cowpox virus BR (CPXV-BR, AF482758). The sequences were obtained from GenBank and aligned by using the default parameters of CLUSTAL W and hand-edited to enhance biologically significant regions. The amino acid positions are shown according to the VACV-WR HA and VGF protein sequences. (.) indicates identity of amino acids, (-) indicates deletions, and (*) indicates the PSTV amino acid substitutions. The box shows the conserved deletion observed in the HA sequence of the Brazilian VACVs. Amino acids substitution found amongst PSTV and other Brazilian VACVs are shown in gray boxes.
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FA.4: Alignment of Passatempo virus (PSTV) hemagluttinin antigen (HA) and vaccinia grown factor (VGF) amino acid inferred sequences with other orthopoxvirus sequences. 1, HA sequence of PSTV (DQ070848) was compared to the region corresponding to amino acids 6 to 309 of vaccinia virus (VACV-WR, AY243312) HA sequence and to HA sequences of Araçatuba virus (ARAV, AY523994); Cantagalo virus (CTGV, AF229247); vaccinia virus IOC (VACV-IOC, AF229248); vaccinia virus Lister (VAVC-LST, AF375124) and cowpox virus Brighton Red (BR) (CPXV-BR, AF482758). 2– The VGF sequence of PSTV (DQ085462) was compared to the region corresponding to amino acids 19 to 135 of vaccinia virus WR (VACV-WR, AY243312) and to the same region of Araçatuba virus (ARAV, AF503170), vaccinia virus Lister (VACV-LST, AY678276); and cowpox virus BR (CPXV-BR, AF482758). The sequences were obtained from GenBank and aligned by using the default parameters of CLUSTAL W and hand-edited to enhance biologically significant regions. The amino acid positions are shown according to the VACV-WR HA and VGF protein sequences. (.) indicates identity of amino acids, (-) indicates deletions, and (*) indicates the PSTV amino acid substitutions. The box shows the conserved deletion observed in the HA sequence of the Brazilian VACVs. Amino acids substitution found amongst PSTV and other Brazilian VACVs are shown in gray boxes.

Mentions: PSTV ha gene sequence was compared to those of ARAV, Cantagalo virus (CTGV) (1,2), VACV-WR, CPXV-BR, VACV Instituto Oswaldo Cruz (VACV-IOC), and VACV Lister (VACV-LST). VACV-IOC and VACV-LST are vaccine strains used in the Brazilian smallpox eradication program (2,6). The PSTV ha gene sequences presented the same 18-nt deletion found in ARAV, CTGV, and VACV-IOC and shared more similarities to ARAV and CTGV homologous sequences. Additionally, 8 amino acid substitutions were unique to PSTV, ARAV, and CTGV. Since this characteristic was not observed in the vaccine strains, an independent origin is suggested. Moreover, PSTV HA differs from that of ARAV and CTGV by 1 and 2 amino acid substitutions, respectively (Figure A4). The percentage of identity between ha, tk, and vgf nucleotide sequences and inferred amino acid sequences of PSTV with CPXV-BR and other VACV strains are presented in the Table. For the tk gene that is highly conserved among VACV, the PSTV nucleotide sequence had 100% identity to ARAV, VACV-LST, and VACV-WR homologous sequences. Additionally, PSTV vgf gene had a 3-nt deletion, corresponding to nt 7,669–7,671 of VACV-WR, causing the loss of 1 isoleucine in a stretch of 4 found in the ARAV and VACV-WR VGF sequences (Figure A4). PSTV VGF also exhibited 2 amino acid substitutions when compared to ARAV VGF sequences.


Passatempo virus, a vaccinia virus strain, Brazil.

Leite JA, Drumond BP, Trindade GS, Lobato ZI, da Fonseca FG, dos SJ, Madureira MC, Guedes MI, Ferreira JM, Bonjardim CA, Ferreira PC, Kroon EG - Emerging Infect. Dis. (2005)

Alignment of Passatempo virus (PSTV) hemagluttinin antigen (HA) and vaccinia grown factor (VGF) amino acid inferred sequences with other orthopoxvirus sequences. 1, HA sequence of PSTV (DQ070848) was compared to the region corresponding to amino acids 6 to 309 of vaccinia virus (VACV-WR, AY243312) HA sequence and to HA sequences of Araçatuba virus (ARAV, AY523994); Cantagalo virus (CTGV, AF229247); vaccinia virus IOC (VACV-IOC, AF229248); vaccinia virus Lister (VAVC-LST, AF375124) and cowpox virus Brighton Red (BR) (CPXV-BR, AF482758). 2– The VGF sequence of PSTV (DQ085462) was compared to the region corresponding to amino acids 19 to 135 of vaccinia virus WR (VACV-WR, AY243312) and to the same region of Araçatuba virus (ARAV, AF503170), vaccinia virus Lister (VACV-LST, AY678276); and cowpox virus BR (CPXV-BR, AF482758). The sequences were obtained from GenBank and aligned by using the default parameters of CLUSTAL W and hand-edited to enhance biologically significant regions. The amino acid positions are shown according to the VACV-WR HA and VGF protein sequences. (.) indicates identity of amino acids, (-) indicates deletions, and (*) indicates the PSTV amino acid substitutions. The box shows the conserved deletion observed in the HA sequence of the Brazilian VACVs. Amino acids substitution found amongst PSTV and other Brazilian VACVs are shown in gray boxes.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3367646&req=5

FA.4: Alignment of Passatempo virus (PSTV) hemagluttinin antigen (HA) and vaccinia grown factor (VGF) amino acid inferred sequences with other orthopoxvirus sequences. 1, HA sequence of PSTV (DQ070848) was compared to the region corresponding to amino acids 6 to 309 of vaccinia virus (VACV-WR, AY243312) HA sequence and to HA sequences of Araçatuba virus (ARAV, AY523994); Cantagalo virus (CTGV, AF229247); vaccinia virus IOC (VACV-IOC, AF229248); vaccinia virus Lister (VAVC-LST, AF375124) and cowpox virus Brighton Red (BR) (CPXV-BR, AF482758). 2– The VGF sequence of PSTV (DQ085462) was compared to the region corresponding to amino acids 19 to 135 of vaccinia virus WR (VACV-WR, AY243312) and to the same region of Araçatuba virus (ARAV, AF503170), vaccinia virus Lister (VACV-LST, AY678276); and cowpox virus BR (CPXV-BR, AF482758). The sequences were obtained from GenBank and aligned by using the default parameters of CLUSTAL W and hand-edited to enhance biologically significant regions. The amino acid positions are shown according to the VACV-WR HA and VGF protein sequences. (.) indicates identity of amino acids, (-) indicates deletions, and (*) indicates the PSTV amino acid substitutions. The box shows the conserved deletion observed in the HA sequence of the Brazilian VACVs. Amino acids substitution found amongst PSTV and other Brazilian VACVs are shown in gray boxes.
Mentions: PSTV ha gene sequence was compared to those of ARAV, Cantagalo virus (CTGV) (1,2), VACV-WR, CPXV-BR, VACV Instituto Oswaldo Cruz (VACV-IOC), and VACV Lister (VACV-LST). VACV-IOC and VACV-LST are vaccine strains used in the Brazilian smallpox eradication program (2,6). The PSTV ha gene sequences presented the same 18-nt deletion found in ARAV, CTGV, and VACV-IOC and shared more similarities to ARAV and CTGV homologous sequences. Additionally, 8 amino acid substitutions were unique to PSTV, ARAV, and CTGV. Since this characteristic was not observed in the vaccine strains, an independent origin is suggested. Moreover, PSTV HA differs from that of ARAV and CTGV by 1 and 2 amino acid substitutions, respectively (Figure A4). The percentage of identity between ha, tk, and vgf nucleotide sequences and inferred amino acid sequences of PSTV with CPXV-BR and other VACV strains are presented in the Table. For the tk gene that is highly conserved among VACV, the PSTV nucleotide sequence had 100% identity to ARAV, VACV-LST, and VACV-WR homologous sequences. Additionally, PSTV vgf gene had a 3-nt deletion, corresponding to nt 7,669–7,671 of VACV-WR, causing the loss of 1 isoleucine in a stretch of 4 found in the ARAV and VACV-WR VGF sequences (Figure A4). PSTV VGF also exhibited 2 amino acid substitutions when compared to ARAV VGF sequences.

Bottom Line: Passatempo virus was isolated during a zoonotic outbreak.Biologic features and molecular characterization of hemagglutinin, thymidine kinase, and vaccinia growth factor genes suggested a vaccinia virus infection, which strengthens the idea of the reemergence and circulation of vaccinia virus in Brazil.Molecular polymorphisms indicated that Passatempo virus is a different isolate.

View Article: PubMed Central - PubMed

Affiliation: Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.

ABSTRACT
Passatempo virus was isolated during a zoonotic outbreak. Biologic features and molecular characterization of hemagglutinin, thymidine kinase, and vaccinia growth factor genes suggested a vaccinia virus infection, which strengthens the idea of the reemergence and circulation of vaccinia virus in Brazil. Molecular polymorphisms indicated that Passatempo virus is a different isolate.

Show MeSH
Related in: MedlinePlus