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Passatempo virus, a vaccinia virus strain, Brazil.

Leite JA, Drumond BP, Trindade GS, Lobato ZI, da Fonseca FG, dos SJ, Madureira MC, Guedes MI, Ferreira JM, Bonjardim CA, Ferreira PC, Kroon EG - Emerging Infect. Dis. (2005)

Bottom Line: Passatempo virus was isolated during a zoonotic outbreak.Biologic features and molecular characterization of hemagglutinin, thymidine kinase, and vaccinia growth factor genes suggested a vaccinia virus infection, which strengthens the idea of the reemergence and circulation of vaccinia virus in Brazil.Molecular polymorphisms indicated that Passatempo virus is a different isolate.

View Article: PubMed Central - PubMed

Affiliation: Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.

ABSTRACT
Passatempo virus was isolated during a zoonotic outbreak. Biologic features and molecular characterization of hemagglutinin, thymidine kinase, and vaccinia growth factor genes suggested a vaccinia virus infection, which strengthens the idea of the reemergence and circulation of vaccinia virus in Brazil. Molecular polymorphisms indicated that Passatempo virus is a different isolate.

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Related in: MedlinePlus

Restriction fragment length polymorphism analysis of the ati gene of viral isolates. ati fragments were amplified by polymerase chain reaction by using primers based on the cowpox virus ati gene nucleotide sequence, followed by XbaI digestion. The amplified DNA was fractionated by electrophoresis on a 1% agarose gel and stained with ethidium bromide. Lanes: 1, vaccinia virus WR; 2, isolate-cow1; 3, isolate-cow2; 4, isolate-cow3; 5, isolate-cow4; 6, isolate-cow5; 7, isolate-calf; 8, cowpox virus BR.
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FA.3: Restriction fragment length polymorphism analysis of the ati gene of viral isolates. ati fragments were amplified by polymerase chain reaction by using primers based on the cowpox virus ati gene nucleotide sequence, followed by XbaI digestion. The amplified DNA was fractionated by electrophoresis on a 1% agarose gel and stained with ethidium bromide. Lanes: 1, vaccinia virus WR; 2, isolate-cow1; 3, isolate-cow2; 4, isolate-cow3; 5, isolate-cow4; 6, isolate-cow5; 7, isolate-calf; 8, cowpox virus BR.

Mentions: Transmission electron microscopy of isolates (6) showed a morphologic pattern typical of orthopoxviruses (Figure A2). No A-type inclusion body (ATI) was seen, reinforcing the conclusion that this virus was likely not a CPXV, but a VACV. Viral DNAs were extracted (6) and used as template for ati gene restriction fragment length polymorphism (RFLP) analysis (7). The ati RFLP patterns of all isolates were identical to those of Ara├žatuba virus (ARAV) (1) and other VACV strains previously isolated in our laboratory (unpub. data); they were similar to those of VACV-WR and completely different from those of CPXV-Brighton Red (BR) (Figure A3). Since all isolates showed the same ati RFLP pattern, one was cloned, purified, titrated (1,6), and named Passatempo virus (PSTV).


Passatempo virus, a vaccinia virus strain, Brazil.

Leite JA, Drumond BP, Trindade GS, Lobato ZI, da Fonseca FG, dos SJ, Madureira MC, Guedes MI, Ferreira JM, Bonjardim CA, Ferreira PC, Kroon EG - Emerging Infect. Dis. (2005)

Restriction fragment length polymorphism analysis of the ati gene of viral isolates. ati fragments were amplified by polymerase chain reaction by using primers based on the cowpox virus ati gene nucleotide sequence, followed by XbaI digestion. The amplified DNA was fractionated by electrophoresis on a 1% agarose gel and stained with ethidium bromide. Lanes: 1, vaccinia virus WR; 2, isolate-cow1; 3, isolate-cow2; 4, isolate-cow3; 5, isolate-cow4; 6, isolate-cow5; 7, isolate-calf; 8, cowpox virus BR.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3367646&req=5

FA.3: Restriction fragment length polymorphism analysis of the ati gene of viral isolates. ati fragments were amplified by polymerase chain reaction by using primers based on the cowpox virus ati gene nucleotide sequence, followed by XbaI digestion. The amplified DNA was fractionated by electrophoresis on a 1% agarose gel and stained with ethidium bromide. Lanes: 1, vaccinia virus WR; 2, isolate-cow1; 3, isolate-cow2; 4, isolate-cow3; 5, isolate-cow4; 6, isolate-cow5; 7, isolate-calf; 8, cowpox virus BR.
Mentions: Transmission electron microscopy of isolates (6) showed a morphologic pattern typical of orthopoxviruses (Figure A2). No A-type inclusion body (ATI) was seen, reinforcing the conclusion that this virus was likely not a CPXV, but a VACV. Viral DNAs were extracted (6) and used as template for ati gene restriction fragment length polymorphism (RFLP) analysis (7). The ati RFLP patterns of all isolates were identical to those of Ara├žatuba virus (ARAV) (1) and other VACV strains previously isolated in our laboratory (unpub. data); they were similar to those of VACV-WR and completely different from those of CPXV-Brighton Red (BR) (Figure A3). Since all isolates showed the same ati RFLP pattern, one was cloned, purified, titrated (1,6), and named Passatempo virus (PSTV).

Bottom Line: Passatempo virus was isolated during a zoonotic outbreak.Biologic features and molecular characterization of hemagglutinin, thymidine kinase, and vaccinia growth factor genes suggested a vaccinia virus infection, which strengthens the idea of the reemergence and circulation of vaccinia virus in Brazil.Molecular polymorphisms indicated that Passatempo virus is a different isolate.

View Article: PubMed Central - PubMed

Affiliation: Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.

ABSTRACT
Passatempo virus was isolated during a zoonotic outbreak. Biologic features and molecular characterization of hemagglutinin, thymidine kinase, and vaccinia growth factor genes suggested a vaccinia virus infection, which strengthens the idea of the reemergence and circulation of vaccinia virus in Brazil. Molecular polymorphisms indicated that Passatempo virus is a different isolate.

Show MeSH
Related in: MedlinePlus