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Passatempo virus, a vaccinia virus strain, Brazil.

Leite JA, Drumond BP, Trindade GS, Lobato ZI, da Fonseca FG, dos SJ, Madureira MC, Guedes MI, Ferreira JM, Bonjardim CA, Ferreira PC, Kroon EG - Emerging Infect. Dis. (2005)

Bottom Line: Passatempo virus was isolated during a zoonotic outbreak.Biologic features and molecular characterization of hemagglutinin, thymidine kinase, and vaccinia growth factor genes suggested a vaccinia virus infection, which strengthens the idea of the reemergence and circulation of vaccinia virus in Brazil.Molecular polymorphisms indicated that Passatempo virus is a different isolate.

View Article: PubMed Central - PubMed

Affiliation: Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.

ABSTRACT
Passatempo virus was isolated during a zoonotic outbreak. Biologic features and molecular characterization of hemagglutinin, thymidine kinase, and vaccinia growth factor genes suggested a vaccinia virus infection, which strengthens the idea of the reemergence and circulation of vaccinia virus in Brazil. Molecular polymorphisms indicated that Passatempo virus is a different isolate.

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Related in: MedlinePlus

Pocks morphology in chorioallantoic membranes. 1 and 2, whitish and opaque pocks could be observed after an incubation period of 72 hours with the Passatempo and vaccinia virus Western Reserve (WR), respectively, on chorioallantoic membrane of 10-day-old embryonated pathogen-free eggs; 3, cowpox virus Brighton Red (BR) formed red hemorrhagic lesions.
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FA.1: Pocks morphology in chorioallantoic membranes. 1 and 2, whitish and opaque pocks could be observed after an incubation period of 72 hours with the Passatempo and vaccinia virus Western Reserve (WR), respectively, on chorioallantoic membrane of 10-day-old embryonated pathogen-free eggs; 3, cowpox virus Brighton Red (BR) formed red hemorrhagic lesions.

Mentions: For virus isolation, crusts were collected from 5 cows and 1 calf, macerated, and added to the chorioallantoic membrane of embryonated eggs (2). The whitish pockmarks produced on chorioallantoic membranes resembled VACV pocks, differing from the red hemorrhagic ones produced by cowpox virus (CPXV) (Figure A1). Blood from affected animals was collected for neutralization assays (5). Serologic cross-reactivity of antibodies to VACV–Western Reserve (WR) strain was detected in all samples, and titers of these serum samples were >640 U/mL (data not shown).


Passatempo virus, a vaccinia virus strain, Brazil.

Leite JA, Drumond BP, Trindade GS, Lobato ZI, da Fonseca FG, dos SJ, Madureira MC, Guedes MI, Ferreira JM, Bonjardim CA, Ferreira PC, Kroon EG - Emerging Infect. Dis. (2005)

Pocks morphology in chorioallantoic membranes. 1 and 2, whitish and opaque pocks could be observed after an incubation period of 72 hours with the Passatempo and vaccinia virus Western Reserve (WR), respectively, on chorioallantoic membrane of 10-day-old embryonated pathogen-free eggs; 3, cowpox virus Brighton Red (BR) formed red hemorrhagic lesions.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3367646&req=5

FA.1: Pocks morphology in chorioallantoic membranes. 1 and 2, whitish and opaque pocks could be observed after an incubation period of 72 hours with the Passatempo and vaccinia virus Western Reserve (WR), respectively, on chorioallantoic membrane of 10-day-old embryonated pathogen-free eggs; 3, cowpox virus Brighton Red (BR) formed red hemorrhagic lesions.
Mentions: For virus isolation, crusts were collected from 5 cows and 1 calf, macerated, and added to the chorioallantoic membrane of embryonated eggs (2). The whitish pockmarks produced on chorioallantoic membranes resembled VACV pocks, differing from the red hemorrhagic ones produced by cowpox virus (CPXV) (Figure A1). Blood from affected animals was collected for neutralization assays (5). Serologic cross-reactivity of antibodies to VACV–Western Reserve (WR) strain was detected in all samples, and titers of these serum samples were >640 U/mL (data not shown).

Bottom Line: Passatempo virus was isolated during a zoonotic outbreak.Biologic features and molecular characterization of hemagglutinin, thymidine kinase, and vaccinia growth factor genes suggested a vaccinia virus infection, which strengthens the idea of the reemergence and circulation of vaccinia virus in Brazil.Molecular polymorphisms indicated that Passatempo virus is a different isolate.

View Article: PubMed Central - PubMed

Affiliation: Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.

ABSTRACT
Passatempo virus was isolated during a zoonotic outbreak. Biologic features and molecular characterization of hemagglutinin, thymidine kinase, and vaccinia growth factor genes suggested a vaccinia virus infection, which strengthens the idea of the reemergence and circulation of vaccinia virus in Brazil. Molecular polymorphisms indicated that Passatempo virus is a different isolate.

Show MeSH
Related in: MedlinePlus