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Intergenogroup recombination in sapoviruses.

Hansman GS, Takeda N, Oka T, Oseto M, Hedlund KO, Katayama K - Emerging Infect. Dis. (2005)

Bottom Line: Analyses of the complete genome sequences led us to identify the first sapovirus intergenogroup recombinant strain.We found that a recombination event occurred at the polymerase and capsid junction.This is the first report of intergenogroup recombination for any calicivirus and highlights a possible route of zoonoses because sapovirus strains that infect pig species belong to genogroup III.

View Article: PubMed Central - PubMed

Affiliation: National Institute of Infectious Diseases, Tokyo, Japan.

ABSTRACT
Sapovirus, a member of the family Caliciviridae, is an etiologic agent of gastroenteritis in humans and pigs. Analyses of the complete genome sequences led us to identify the first sapovirus intergenogroup recombinant strain. Phylogenetic analysis of the nonstructural region (i.e., genome start to capsid start) grouped this strain into genogroup II, whereas the structural region (i.e., capsid start to genome end) grouped this strain into genogroup IV. We found that a recombination event occurred at the polymerase and capsid junction. This is the first report of intergenogroup recombination for any calicivirus and highlights a possible route of zoonoses because sapovirus strains that infect pig species belong to genogroup III.

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Related in: MedlinePlus

Phylogenetic analysis of (A) the nonstructural region (i.e., genome start to capsid start) and (B) the structural region (i.e., capsid start to genome end), showing the different genogroups. The numbers on each branch indicate the bootstrap values for the genotype. Bootstrap values >950 were considered significant for the grouping (10). The scale represents nucleotide substitutions per site. GenBank accession numbers are as follows: Mc10, AY237420; Manchester, X86560; Dresden, AY694184; SW278, DQ125333; Ehime1107, DQ058829; NK24, AY646856; C12, AY603425; Bristol, AJ249939; Mc2, AY237419; PEC, AF182760; and SK15, AY646855.
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Figure 1: Phylogenetic analysis of (A) the nonstructural region (i.e., genome start to capsid start) and (B) the structural region (i.e., capsid start to genome end), showing the different genogroups. The numbers on each branch indicate the bootstrap values for the genotype. Bootstrap values >950 were considered significant for the grouping (10). The scale represents nucleotide substitutions per site. GenBank accession numbers are as follows: Mc10, AY237420; Manchester, X86560; Dresden, AY694184; SW278, DQ125333; Ehime1107, DQ058829; NK24, AY646856; C12, AY603425; Bristol, AJ249939; Mc2, AY237419; PEC, AF182760; and SK15, AY646855.

Mentions: Based on the classification scheme of either the partial or complete capsid sequences in our previous studies, we grouped Manchester into GI; Bristol, Mc2, Mc10, C12, and SK15 into GII; PEC into GIII; and NK24 into GV (6,8,9). For this study and on the basis of the structural region (i.e., capsid start to genome end), we grouped Manchester into GI; Mc2, Bristol, Mc10, C12; and SK15 into GII; PEC into GIII; SW278 and Ehime1107 into GIV, and NK24 into GV (Figure 1). These genogroups were not maintained when we analyzed the nonstructural region (i.e., genome start to capsid start). We found that SW278 and Ehime1107 clustered into GII for the nonstructural region–based grouping but clustered into GIV for the structural region–based grouping. All genogroups were supported by bootstrap values (10), except for the structural region–based grouping of GI, which had a slightly lower value of 897. Nevertheless, these results indicate that the nonstructural region of SW278 and Ehime1107, i.e., a GII sequence, did not belong to a distinct genogroup, unlike their structural region, which belonged to a distinct genogroup (proposed as GIV). Comparisons of the complete genome sequences showed that SW278 and Ehime1107 shared >97% nucleotide identity and likely represented the same strain, although it was isolated from different countries; however, the lengths were different. Either SW278 or Ehime1107 had a 10-nucleotide insertion or deletion in the nontranslated region at the 3´ terminus. A number of closely matching partial sequences to SW278 and Ehime1107, which included both the polymerase and capsid gene, were available on the database, which indicates the circulation of similar strains in other countries.


Intergenogroup recombination in sapoviruses.

Hansman GS, Takeda N, Oka T, Oseto M, Hedlund KO, Katayama K - Emerging Infect. Dis. (2005)

Phylogenetic analysis of (A) the nonstructural region (i.e., genome start to capsid start) and (B) the structural region (i.e., capsid start to genome end), showing the different genogroups. The numbers on each branch indicate the bootstrap values for the genotype. Bootstrap values >950 were considered significant for the grouping (10). The scale represents nucleotide substitutions per site. GenBank accession numbers are as follows: Mc10, AY237420; Manchester, X86560; Dresden, AY694184; SW278, DQ125333; Ehime1107, DQ058829; NK24, AY646856; C12, AY603425; Bristol, AJ249939; Mc2, AY237419; PEC, AF182760; and SK15, AY646855.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3367643&req=5

Figure 1: Phylogenetic analysis of (A) the nonstructural region (i.e., genome start to capsid start) and (B) the structural region (i.e., capsid start to genome end), showing the different genogroups. The numbers on each branch indicate the bootstrap values for the genotype. Bootstrap values >950 were considered significant for the grouping (10). The scale represents nucleotide substitutions per site. GenBank accession numbers are as follows: Mc10, AY237420; Manchester, X86560; Dresden, AY694184; SW278, DQ125333; Ehime1107, DQ058829; NK24, AY646856; C12, AY603425; Bristol, AJ249939; Mc2, AY237419; PEC, AF182760; and SK15, AY646855.
Mentions: Based on the classification scheme of either the partial or complete capsid sequences in our previous studies, we grouped Manchester into GI; Bristol, Mc2, Mc10, C12, and SK15 into GII; PEC into GIII; and NK24 into GV (6,8,9). For this study and on the basis of the structural region (i.e., capsid start to genome end), we grouped Manchester into GI; Mc2, Bristol, Mc10, C12; and SK15 into GII; PEC into GIII; SW278 and Ehime1107 into GIV, and NK24 into GV (Figure 1). These genogroups were not maintained when we analyzed the nonstructural region (i.e., genome start to capsid start). We found that SW278 and Ehime1107 clustered into GII for the nonstructural region–based grouping but clustered into GIV for the structural region–based grouping. All genogroups were supported by bootstrap values (10), except for the structural region–based grouping of GI, which had a slightly lower value of 897. Nevertheless, these results indicate that the nonstructural region of SW278 and Ehime1107, i.e., a GII sequence, did not belong to a distinct genogroup, unlike their structural region, which belonged to a distinct genogroup (proposed as GIV). Comparisons of the complete genome sequences showed that SW278 and Ehime1107 shared >97% nucleotide identity and likely represented the same strain, although it was isolated from different countries; however, the lengths were different. Either SW278 or Ehime1107 had a 10-nucleotide insertion or deletion in the nontranslated region at the 3´ terminus. A number of closely matching partial sequences to SW278 and Ehime1107, which included both the polymerase and capsid gene, were available on the database, which indicates the circulation of similar strains in other countries.

Bottom Line: Analyses of the complete genome sequences led us to identify the first sapovirus intergenogroup recombinant strain.We found that a recombination event occurred at the polymerase and capsid junction.This is the first report of intergenogroup recombination for any calicivirus and highlights a possible route of zoonoses because sapovirus strains that infect pig species belong to genogroup III.

View Article: PubMed Central - PubMed

Affiliation: National Institute of Infectious Diseases, Tokyo, Japan.

ABSTRACT
Sapovirus, a member of the family Caliciviridae, is an etiologic agent of gastroenteritis in humans and pigs. Analyses of the complete genome sequences led us to identify the first sapovirus intergenogroup recombinant strain. Phylogenetic analysis of the nonstructural region (i.e., genome start to capsid start) grouped this strain into genogroup II, whereas the structural region (i.e., capsid start to genome end) grouped this strain into genogroup IV. We found that a recombination event occurred at the polymerase and capsid junction. This is the first report of intergenogroup recombination for any calicivirus and highlights a possible route of zoonoses because sapovirus strains that infect pig species belong to genogroup III.

Show MeSH
Related in: MedlinePlus