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Human Rickettsia felis infection, Canary Islands, Spain.

Pérez-Arellano JL, Fenollar F, Angel-Moreno A, Bolaños M, Hernández M, Santana E, Hemmersbach-Miller M, Martín AM, Raoult D - Emerging Infect. Dis. (2005)

Bottom Line: We report the first cases of human infection by Rickettsia felis in the Canary Islands.Antibodies against R. felis were found in 5 adsorbed serum samples from 44 patients with clinically suspected rickettsiosis by Western blot serology.Fleas from 1 patient's dog were positive for R. felis by polymerase chain reaction.

View Article: PubMed Central - PubMed

Affiliation: Hospital Universitario Insular de Las Palmas, Canary Islands, Spain.

ABSTRACT
We report the first cases of human infection by Rickettsia felis in the Canary Islands. Antibodies against R. felis were found in 5 adsorbed serum samples from 44 patients with clinically suspected rickettsiosis by Western blot serology. Fleas from 1 patient's dog were positive for R. felis by polymerase chain reaction.

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Results of Western blot performed with serum samples from patient 5 with Rickettsia felis infection and patient 10 with R. typhi infection. Molecular masses (in kilodaltons) are given to the left of panels. A) Patient with R. felis infection; a, untreated serum analyzed by using R. conorii (lane 1), R. typhi (lane 2), and R. felis (lane 3); b, R. felis–adsorbed serum analyzed by using R. conorii (lane 1), R. typhi (lane 2), R. felis (lane 3); all antibodies were removed; c, R. typhi–adsorbed serum analyzed by using R. typhi (lane 1) and R. felis (lane 2); antibodies to R. felis remained; d, R. conorii–adsorbed serum analyzed by using R. conorii (lane 1), R. typhi (lane 2), R. felis (lane 3); antibodies to R. felis remained. B) Patient with murine typhus; e, untreated serum analyzed by using R. typhi (lane 1) and R. felis (lane 2); f, R. felis–adsorbed serum analyzed by using R. typhi (lane 1) and R. felis (lane 2); antibodies to R. typhi remained; g, R. typhi–adsorbed serum analyzed by using R. typhi (lane 1) and R. felis (lane 2); all antibodies were removed.
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Figure 1: Results of Western blot performed with serum samples from patient 5 with Rickettsia felis infection and patient 10 with R. typhi infection. Molecular masses (in kilodaltons) are given to the left of panels. A) Patient with R. felis infection; a, untreated serum analyzed by using R. conorii (lane 1), R. typhi (lane 2), and R. felis (lane 3); b, R. felis–adsorbed serum analyzed by using R. conorii (lane 1), R. typhi (lane 2), R. felis (lane 3); all antibodies were removed; c, R. typhi–adsorbed serum analyzed by using R. typhi (lane 1) and R. felis (lane 2); antibodies to R. felis remained; d, R. conorii–adsorbed serum analyzed by using R. conorii (lane 1), R. typhi (lane 2), R. felis (lane 3); antibodies to R. felis remained. B) Patient with murine typhus; e, untreated serum analyzed by using R. typhi (lane 1) and R. felis (lane 2); f, R. felis–adsorbed serum analyzed by using R. typhi (lane 1) and R. felis (lane 2); antibodies to R. typhi remained; g, R. typhi–adsorbed serum analyzed by using R. typhi (lane 1) and R. felis (lane 2); all antibodies were removed.

Mentions: Western blots performed with unadsorbed and adsorbed sera are represented in the Figure. Features of patients are indicated in the Appendix. Some differences were found between groups. The interval between the beginning of clinical signs and symptoms and evaluation was significantly more prolonged in the R. felis group than others. In the R. typhi group, odynophagia, cough, and rash were more frequent. When we compared biologic data, no difference was observed between R. typhi and R. felis groups, except for milder hypertransaminasemia in the latter group. Finally, 2 PCR products were obtained and sequenced from 2 fleas. Both sequences were 100% similar to R. felis citrate synthase gene in GenBank accession no. AF210692. No fleas were positive for R. typhi. Amplification was unsuccessful in all negative controls.


Human Rickettsia felis infection, Canary Islands, Spain.

Pérez-Arellano JL, Fenollar F, Angel-Moreno A, Bolaños M, Hernández M, Santana E, Hemmersbach-Miller M, Martín AM, Raoult D - Emerging Infect. Dis. (2005)

Results of Western blot performed with serum samples from patient 5 with Rickettsia felis infection and patient 10 with R. typhi infection. Molecular masses (in kilodaltons) are given to the left of panels. A) Patient with R. felis infection; a, untreated serum analyzed by using R. conorii (lane 1), R. typhi (lane 2), and R. felis (lane 3); b, R. felis–adsorbed serum analyzed by using R. conorii (lane 1), R. typhi (lane 2), R. felis (lane 3); all antibodies were removed; c, R. typhi–adsorbed serum analyzed by using R. typhi (lane 1) and R. felis (lane 2); antibodies to R. felis remained; d, R. conorii–adsorbed serum analyzed by using R. conorii (lane 1), R. typhi (lane 2), R. felis (lane 3); antibodies to R. felis remained. B) Patient with murine typhus; e, untreated serum analyzed by using R. typhi (lane 1) and R. felis (lane 2); f, R. felis–adsorbed serum analyzed by using R. typhi (lane 1) and R. felis (lane 2); antibodies to R. typhi remained; g, R. typhi–adsorbed serum analyzed by using R. typhi (lane 1) and R. felis (lane 2); all antibodies were removed.
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Related In: Results  -  Collection

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Figure 1: Results of Western blot performed with serum samples from patient 5 with Rickettsia felis infection and patient 10 with R. typhi infection. Molecular masses (in kilodaltons) are given to the left of panels. A) Patient with R. felis infection; a, untreated serum analyzed by using R. conorii (lane 1), R. typhi (lane 2), and R. felis (lane 3); b, R. felis–adsorbed serum analyzed by using R. conorii (lane 1), R. typhi (lane 2), R. felis (lane 3); all antibodies were removed; c, R. typhi–adsorbed serum analyzed by using R. typhi (lane 1) and R. felis (lane 2); antibodies to R. felis remained; d, R. conorii–adsorbed serum analyzed by using R. conorii (lane 1), R. typhi (lane 2), R. felis (lane 3); antibodies to R. felis remained. B) Patient with murine typhus; e, untreated serum analyzed by using R. typhi (lane 1) and R. felis (lane 2); f, R. felis–adsorbed serum analyzed by using R. typhi (lane 1) and R. felis (lane 2); antibodies to R. typhi remained; g, R. typhi–adsorbed serum analyzed by using R. typhi (lane 1) and R. felis (lane 2); all antibodies were removed.
Mentions: Western blots performed with unadsorbed and adsorbed sera are represented in the Figure. Features of patients are indicated in the Appendix. Some differences were found between groups. The interval between the beginning of clinical signs and symptoms and evaluation was significantly more prolonged in the R. felis group than others. In the R. typhi group, odynophagia, cough, and rash were more frequent. When we compared biologic data, no difference was observed between R. typhi and R. felis groups, except for milder hypertransaminasemia in the latter group. Finally, 2 PCR products were obtained and sequenced from 2 fleas. Both sequences were 100% similar to R. felis citrate synthase gene in GenBank accession no. AF210692. No fleas were positive for R. typhi. Amplification was unsuccessful in all negative controls.

Bottom Line: We report the first cases of human infection by Rickettsia felis in the Canary Islands.Antibodies against R. felis were found in 5 adsorbed serum samples from 44 patients with clinically suspected rickettsiosis by Western blot serology.Fleas from 1 patient's dog were positive for R. felis by polymerase chain reaction.

View Article: PubMed Central - PubMed

Affiliation: Hospital Universitario Insular de Las Palmas, Canary Islands, Spain.

ABSTRACT
We report the first cases of human infection by Rickettsia felis in the Canary Islands. Antibodies against R. felis were found in 5 adsorbed serum samples from 44 patients with clinically suspected rickettsiosis by Western blot serology. Fleas from 1 patient's dog were positive for R. felis by polymerase chain reaction.

Show MeSH
Related in: MedlinePlus