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Porcine noroviruses related to human noroviruses.

Wang QH, Han MG, Cheetham S, Souza M, Funk JA, Saif LJ - Emerging Infect. Dis. (2005)

Bottom Line: Six samples were positive for NoV.Based on sequence analysis of 3 kb on the 3' end of 5 porcine NoVs, 3 genotypes in GII and a potential recombinant were identified.These results raise concerns of whether subclinically infected adult swine may be reservoirs of new human NoVs or if porcine/human GII recombinants could emerge.

View Article: PubMed Central - PubMed

Affiliation: Food Animal Health Research Program, The Ohio State University, Wooster, Ohio 44691, USA.

ABSTRACT
Detection of genogroup II (GII) norovirus (NoV) RNA from adult pigs in Japan and Europe and GII NoV antibodies in US swine raises public health concerns about zoonotic transmission of porcine NoVs to humans, although no NoVs have been detected in US swine. To detect porcine NoVs and to investigate their genetic diversity and relatedness to human NoVs, 275 fecal samples from normal US adult swine were screened by reverse transcription-polymerase chain reaction with calicivirus universal primers. Six samples were positive for NoV. Based on sequence analysis of 3 kb on the 3' end of 5 porcine NoVs, 3 genotypes in GII and a potential recombinant were identified. One genotype of porcine NoVs was genetically and antigenically related to human NoVs and replicated in gnotobiotic pigs. These results raise concerns of whether subclinically infected adult swine may be reservoirs of new human NoVs or if porcine/human GII recombinants could emerge.

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Related in: MedlinePlus

Neighbor-joining phylogenetic tree of genogroup II noroviruses (NoVs) based on the complete capsid region. The 5 newly identified porcine NoV strains are in boldface. Genogroups (G) and genotypes (numbers after G) are indicated. The human NoV GI-1/Norwalk and GIV/Alphatron strains were used as outgroup controls.
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Figure 1: Neighbor-joining phylogenetic tree of genogroup II noroviruses (NoVs) based on the complete capsid region. The 5 newly identified porcine NoV strains are in boldface. Genogroups (G) and genotypes (numbers after G) are indicated. The human NoV GI-1/Norwalk and GIV/Alphatron strains were used as outgroup controls.

Mentions: The amino acid identity of the predicted complete and S and P domains of the capsid protein of the 5 porcine NoVs, the previously reported porcine NoVs (Sw43 and Sw918), and representative human, bovine, and murine NoV strains is summarized in Table 3. In the complete capsid, the QW48 strain was most closely related to the porcine NoV prototype Sw43 strain (98% amino acid identity); the QW170 and QW218 strains shared the highest amino acid identities (81%) to porcine Sw43 and Sw918 strains; the QW101 and QW125 strains showed the highest amino acid identity to human GII-3/Mexico (71.4%), then to human GII-6/Baltimore (71.0%), porcine QW218 (71.0%), and porcine Sw43 (70.6%) strains. The S and P domains of these NoVs showed similar relationships. A neighbor-joining phylogenetic tree based on the amino acid sequences of the complete capsids (Figure 1) showed that QW48 grouped with Sw43 and Sw918 strains into GII-11 and that QW170 and QW218 formed a new genotype (GII-19), which was closer to porcine than to human strains. However, QW101 and 125 formed a new genotype (GII-18) between human and porcine GII NoVs.


Porcine noroviruses related to human noroviruses.

Wang QH, Han MG, Cheetham S, Souza M, Funk JA, Saif LJ - Emerging Infect. Dis. (2005)

Neighbor-joining phylogenetic tree of genogroup II noroviruses (NoVs) based on the complete capsid region. The 5 newly identified porcine NoV strains are in boldface. Genogroups (G) and genotypes (numbers after G) are indicated. The human NoV GI-1/Norwalk and GIV/Alphatron strains were used as outgroup controls.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3367634&req=5

Figure 1: Neighbor-joining phylogenetic tree of genogroup II noroviruses (NoVs) based on the complete capsid region. The 5 newly identified porcine NoV strains are in boldface. Genogroups (G) and genotypes (numbers after G) are indicated. The human NoV GI-1/Norwalk and GIV/Alphatron strains were used as outgroup controls.
Mentions: The amino acid identity of the predicted complete and S and P domains of the capsid protein of the 5 porcine NoVs, the previously reported porcine NoVs (Sw43 and Sw918), and representative human, bovine, and murine NoV strains is summarized in Table 3. In the complete capsid, the QW48 strain was most closely related to the porcine NoV prototype Sw43 strain (98% amino acid identity); the QW170 and QW218 strains shared the highest amino acid identities (81%) to porcine Sw43 and Sw918 strains; the QW101 and QW125 strains showed the highest amino acid identity to human GII-3/Mexico (71.4%), then to human GII-6/Baltimore (71.0%), porcine QW218 (71.0%), and porcine Sw43 (70.6%) strains. The S and P domains of these NoVs showed similar relationships. A neighbor-joining phylogenetic tree based on the amino acid sequences of the complete capsids (Figure 1) showed that QW48 grouped with Sw43 and Sw918 strains into GII-11 and that QW170 and QW218 formed a new genotype (GII-19), which was closer to porcine than to human strains. However, QW101 and 125 formed a new genotype (GII-18) between human and porcine GII NoVs.

Bottom Line: Six samples were positive for NoV.Based on sequence analysis of 3 kb on the 3' end of 5 porcine NoVs, 3 genotypes in GII and a potential recombinant were identified.These results raise concerns of whether subclinically infected adult swine may be reservoirs of new human NoVs or if porcine/human GII recombinants could emerge.

View Article: PubMed Central - PubMed

Affiliation: Food Animal Health Research Program, The Ohio State University, Wooster, Ohio 44691, USA.

ABSTRACT
Detection of genogroup II (GII) norovirus (NoV) RNA from adult pigs in Japan and Europe and GII NoV antibodies in US swine raises public health concerns about zoonotic transmission of porcine NoVs to humans, although no NoVs have been detected in US swine. To detect porcine NoVs and to investigate their genetic diversity and relatedness to human NoVs, 275 fecal samples from normal US adult swine were screened by reverse transcription-polymerase chain reaction with calicivirus universal primers. Six samples were positive for NoV. Based on sequence analysis of 3 kb on the 3' end of 5 porcine NoVs, 3 genotypes in GII and a potential recombinant were identified. One genotype of porcine NoVs was genetically and antigenically related to human NoVs and replicated in gnotobiotic pigs. These results raise concerns of whether subclinically infected adult swine may be reservoirs of new human NoVs or if porcine/human GII recombinants could emerge.

Show MeSH
Related in: MedlinePlus