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Antimicrobial-drug susceptibility of human and animal Salmonella typhimurium, Minnesota, 1997-2003.

Wedel SD, Bender JB, Leano FT, Boxrud DJ, Hedberg C, Smith KE - Emerging Infect. Dis. (2005)

Bottom Line: Resistance to ceftriaxone and nalidixic acid increased, primarily among clonal group A/ACSSuT isolates.These data support the hypothesis that food animals are the primary reservoir of multidrug-resistant S.Typhimurium.

View Article: PubMed Central - PubMed

Affiliation: Acute Disease Investigation and Control Section, Minnesota Department of Health, Minneapolis, Minnesota 55164-0975, USA. stephanie.wedel@health.state.mn.us

ABSTRACT
We compared antimicrobial resistance phenotypes and pulsed-field gel electrophoresis (PFGE) subtypes of 1,028 human and 716 animal Salmonella enterica serotype Typhimurium isolates from Minnesota from 1997 to 2003. Overall, 29% of human isolates were multidrug resistant. Predominant phenotypes included resistance to ampicillin, chloramphenicol or kanamycin, streptomycin, sulfisoxazole, and tetracycline (ACSSuT or AKSSuT). Most human multidrug-resistant isolates belonged to PFGE clonal group A, characterized by ACSSuT resistance (64%), or clonal group B, characterized by AKSSuT resistance (19%). Most animal isolates were from cattle (n = 358) or swine (n = 251). Eighty-one percent were multidrug resistant; of these, 54% were at least resistance phenotype ACSSuT, and 43% were at least AKSSuT. More than 80% of multidrug-resistant isolates had a clonal group A or B subtype. Resistance to ceftriaxone and nalidixic acid increased, primarily among clonal group A/ACSSuT isolates. Clonal group B/AKSSuT isolates decreased over time. These data support the hypothesis that food animals are the primary reservoir of multidrug-resistant S. Typhimurium.

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Distribution of Salmonella enterica serovar Typhimurium clonal group A pulsed-field gel electrophoresis (PFGE) subtypes and clonal group B PFGE subtypes among clinical isolates from humans and animals by species, Minnesota, 1997–2003. Clonal group A subtypes were <3 bands different from subtype TM5b by PFGE and were associated with resistance to ampicillin, chloramphenicol, streptomycin, sulfisoxazole, and tetracycline. Clonal group B PFGE subtypes were <3 bands different from subtype TM54 and were associated with resistance to ampicillin, kanamycin, streptomycin, sulfisoxazole, and tetracycline. H, C, and S indicate human, cattle, and swine isolates, respectively.
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Figure 2: Distribution of Salmonella enterica serovar Typhimurium clonal group A pulsed-field gel electrophoresis (PFGE) subtypes and clonal group B PFGE subtypes among clinical isolates from humans and animals by species, Minnesota, 1997–2003. Clonal group A subtypes were <3 bands different from subtype TM5b by PFGE and were associated with resistance to ampicillin, chloramphenicol, streptomycin, sulfisoxazole, and tetracycline. Clonal group B PFGE subtypes were <3 bands different from subtype TM54 and were associated with resistance to ampicillin, kanamycin, streptomycin, sulfisoxazole, and tetracycline. H, C, and S indicate human, cattle, and swine isolates, respectively.

Mentions: We identified 271 unique PFGE subtypes among the 1,028 human S. Typhimurium isolates (median 63 subtypes/year, range 52–72). The 10 most common subtypes accounted for 509 (50%) isolates. CGA was composed of 31 PFGE subtypes. These subtypes accounted for 217 (21%) of all 1,028 human isolates, 188 (64%) of 296 MDR isolates, and 181 (83%) of 217 isolates that were at least R-type ACSSuT, including 12 isolates that were at least R-type ACKSSuT (Table 2, Figures 2 and 3).


Antimicrobial-drug susceptibility of human and animal Salmonella typhimurium, Minnesota, 1997-2003.

Wedel SD, Bender JB, Leano FT, Boxrud DJ, Hedberg C, Smith KE - Emerging Infect. Dis. (2005)

Distribution of Salmonella enterica serovar Typhimurium clonal group A pulsed-field gel electrophoresis (PFGE) subtypes and clonal group B PFGE subtypes among clinical isolates from humans and animals by species, Minnesota, 1997–2003. Clonal group A subtypes were <3 bands different from subtype TM5b by PFGE and were associated with resistance to ampicillin, chloramphenicol, streptomycin, sulfisoxazole, and tetracycline. Clonal group B PFGE subtypes were <3 bands different from subtype TM54 and were associated with resistance to ampicillin, kanamycin, streptomycin, sulfisoxazole, and tetracycline. H, C, and S indicate human, cattle, and swine isolates, respectively.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3367623&req=5

Figure 2: Distribution of Salmonella enterica serovar Typhimurium clonal group A pulsed-field gel electrophoresis (PFGE) subtypes and clonal group B PFGE subtypes among clinical isolates from humans and animals by species, Minnesota, 1997–2003. Clonal group A subtypes were <3 bands different from subtype TM5b by PFGE and were associated with resistance to ampicillin, chloramphenicol, streptomycin, sulfisoxazole, and tetracycline. Clonal group B PFGE subtypes were <3 bands different from subtype TM54 and were associated with resistance to ampicillin, kanamycin, streptomycin, sulfisoxazole, and tetracycline. H, C, and S indicate human, cattle, and swine isolates, respectively.
Mentions: We identified 271 unique PFGE subtypes among the 1,028 human S. Typhimurium isolates (median 63 subtypes/year, range 52–72). The 10 most common subtypes accounted for 509 (50%) isolates. CGA was composed of 31 PFGE subtypes. These subtypes accounted for 217 (21%) of all 1,028 human isolates, 188 (64%) of 296 MDR isolates, and 181 (83%) of 217 isolates that were at least R-type ACSSuT, including 12 isolates that were at least R-type ACKSSuT (Table 2, Figures 2 and 3).

Bottom Line: Resistance to ceftriaxone and nalidixic acid increased, primarily among clonal group A/ACSSuT isolates.These data support the hypothesis that food animals are the primary reservoir of multidrug-resistant S.Typhimurium.

View Article: PubMed Central - PubMed

Affiliation: Acute Disease Investigation and Control Section, Minnesota Department of Health, Minneapolis, Minnesota 55164-0975, USA. stephanie.wedel@health.state.mn.us

ABSTRACT
We compared antimicrobial resistance phenotypes and pulsed-field gel electrophoresis (PFGE) subtypes of 1,028 human and 716 animal Salmonella enterica serotype Typhimurium isolates from Minnesota from 1997 to 2003. Overall, 29% of human isolates were multidrug resistant. Predominant phenotypes included resistance to ampicillin, chloramphenicol or kanamycin, streptomycin, sulfisoxazole, and tetracycline (ACSSuT or AKSSuT). Most human multidrug-resistant isolates belonged to PFGE clonal group A, characterized by ACSSuT resistance (64%), or clonal group B, characterized by AKSSuT resistance (19%). Most animal isolates were from cattle (n = 358) or swine (n = 251). Eighty-one percent were multidrug resistant; of these, 54% were at least resistance phenotype ACSSuT, and 43% were at least AKSSuT. More than 80% of multidrug-resistant isolates had a clonal group A or B subtype. Resistance to ceftriaxone and nalidixic acid increased, primarily among clonal group A/ACSSuT isolates. Clonal group B/AKSSuT isolates decreased over time. These data support the hypothesis that food animals are the primary reservoir of multidrug-resistant S. Typhimurium.

Show MeSH
Related in: MedlinePlus