Limits...
Bifidobacterium infantis 35624 protects against salmonella-induced reductions in digestive enzyme activity in mice by attenuation of the host inflammatory response.

Symonds EL, O'Mahony C, Lapthorne S, O'Mahony D, Sharry JM, O'Mahony L, Shanahan F - Clin Transl Gastroenterol (2012)

Bottom Line: Typhimurium infection significantly reduced the activity of all brush border enzymes in a dose- and time-dependent manner (P<0.05).Salmonella infection reduces the small intestinal brush border enzyme activity in mice, with the level of reduction and associated weight loss increasing with dose and duration of infection.B. longum subsp. infantis 35624 treatment attenuated the effect of Salmonella infection on brush border enzyme activity and weight loss, which may be due to modulation of the host immune response.

View Article: PubMed Central - PubMed

Affiliation: Alimentary Pharmabiotic Centre, National University Ireland, Cork, Ireland.

ABSTRACT

Objectives: Salmonella-induced damage to the small intestine may decrease the villi-associated enzyme activity, causing malabsorption of nutrients and diarrhea, and thus contribute to the symptoms of infection. The objective of this study was to determine the mechanism by which different doses and durations of Salmonella infection and lipopolysaccharide (LPS) affect brush border enzyme activity in the mouse, and to determine if the probiotic Bifidobacterium longum subspecies infantis 35624 could attenuate the intestinal damage.

Methods: BALB/c mice were challenged with Salmonella enterica serovar Typhimurium UK1 at various doses (10(2)-10(8) colony-forming unit (CFU)) and durations (10(6) CFU for 1-6 days). Mice were also treated with B. longum subsp. infantis 35624 for 2 weeks before and during a 6-day S. Typhimurium challenge (10(6) CFU), or before injection of LPS. The small intestine was assessed for morphological changes, mRNA expression of cytokines, and activity of the brush border enzymes sucrase-isomaltase, maltase, and alkaline phosphatase.

Results: S. Typhimurium infection significantly reduced the activity of all brush border enzymes in a dose- and time-dependent manner (P<0.05). This also occurred following injection of LPS. Pre-treatment with B. longum subsp. infantis 35624 prevented weight loss, protected brush border enzyme activity, reduced the small intestinal damage, and inhibited the increase in interleukin (IL)-10 and IL-8 expression due to Salmonella challenge.

Conclusions: Salmonella infection reduces the small intestinal brush border enzyme activity in mice, with the level of reduction and associated weight loss increasing with dose and duration of infection. B. longum subsp. infantis 35624 treatment attenuated the effect of Salmonella infection on brush border enzyme activity and weight loss, which may be due to modulation of the host immune response.

No MeSH data available.


Related in: MedlinePlus

Weight change in non-infected mice (diamonds), S. Typhimurium UK1-infected mice with B. longum subsp. infantis 35624 pre-treatment (squares), and S. Typhimurium UK1-infected mice (broken line). The values are mean percentage weight change compared with the baseline weight±s.e. *P<0.05 compared with non-infected mice. N=6 per group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3367613&req=5

fig5: Weight change in non-infected mice (diamonds), S. Typhimurium UK1-infected mice with B. longum subsp. infantis 35624 pre-treatment (squares), and S. Typhimurium UK1-infected mice (broken line). The values are mean percentage weight change compared with the baseline weight±s.e. *P<0.05 compared with non-infected mice. N=6 per group.

Mentions: The jejunum is an area of high nutrient uptake, and as greater physiological alterations were occurring here following infection, we only assessed this site for the subsequent probiotic treatment studies. Salmonella infection caused weight loss, which was evident from day 3 post-infection (P<0.05; Figure 5). Pre-treatment with B. longum subsp. infantis 35624 prevented the Salmonella-associated weight loss, with no differences between weight of this group and non-infected mice. B. longum subsp. infantis 35624 also attenuated the reduction in enzyme activity, with sucrase–isomaltase activity significantly greater than the non-treated Salmonella-infected mice (P<0.005; Figure 6). B. longum subsp. infantis 35624 reduced the Salmonella-induced decrease in the villi length (villi length as the percentage of control: Salmonella=71.3±5.3% B. longum subsp. infantis 35624=87.7±6.6%), but did not alter the levels of Salmonella in the spleen, liver, or small intestine (Table 4; P>0.05).


Bifidobacterium infantis 35624 protects against salmonella-induced reductions in digestive enzyme activity in mice by attenuation of the host inflammatory response.

Symonds EL, O'Mahony C, Lapthorne S, O'Mahony D, Sharry JM, O'Mahony L, Shanahan F - Clin Transl Gastroenterol (2012)

Weight change in non-infected mice (diamonds), S. Typhimurium UK1-infected mice with B. longum subsp. infantis 35624 pre-treatment (squares), and S. Typhimurium UK1-infected mice (broken line). The values are mean percentage weight change compared with the baseline weight±s.e. *P<0.05 compared with non-infected mice. N=6 per group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3367613&req=5

fig5: Weight change in non-infected mice (diamonds), S. Typhimurium UK1-infected mice with B. longum subsp. infantis 35624 pre-treatment (squares), and S. Typhimurium UK1-infected mice (broken line). The values are mean percentage weight change compared with the baseline weight±s.e. *P<0.05 compared with non-infected mice. N=6 per group.
Mentions: The jejunum is an area of high nutrient uptake, and as greater physiological alterations were occurring here following infection, we only assessed this site for the subsequent probiotic treatment studies. Salmonella infection caused weight loss, which was evident from day 3 post-infection (P<0.05; Figure 5). Pre-treatment with B. longum subsp. infantis 35624 prevented the Salmonella-associated weight loss, with no differences between weight of this group and non-infected mice. B. longum subsp. infantis 35624 also attenuated the reduction in enzyme activity, with sucrase–isomaltase activity significantly greater than the non-treated Salmonella-infected mice (P<0.005; Figure 6). B. longum subsp. infantis 35624 reduced the Salmonella-induced decrease in the villi length (villi length as the percentage of control: Salmonella=71.3±5.3% B. longum subsp. infantis 35624=87.7±6.6%), but did not alter the levels of Salmonella in the spleen, liver, or small intestine (Table 4; P>0.05).

Bottom Line: Typhimurium infection significantly reduced the activity of all brush border enzymes in a dose- and time-dependent manner (P<0.05).Salmonella infection reduces the small intestinal brush border enzyme activity in mice, with the level of reduction and associated weight loss increasing with dose and duration of infection.B. longum subsp. infantis 35624 treatment attenuated the effect of Salmonella infection on brush border enzyme activity and weight loss, which may be due to modulation of the host immune response.

View Article: PubMed Central - PubMed

Affiliation: Alimentary Pharmabiotic Centre, National University Ireland, Cork, Ireland.

ABSTRACT

Objectives: Salmonella-induced damage to the small intestine may decrease the villi-associated enzyme activity, causing malabsorption of nutrients and diarrhea, and thus contribute to the symptoms of infection. The objective of this study was to determine the mechanism by which different doses and durations of Salmonella infection and lipopolysaccharide (LPS) affect brush border enzyme activity in the mouse, and to determine if the probiotic Bifidobacterium longum subspecies infantis 35624 could attenuate the intestinal damage.

Methods: BALB/c mice were challenged with Salmonella enterica serovar Typhimurium UK1 at various doses (10(2)-10(8) colony-forming unit (CFU)) and durations (10(6) CFU for 1-6 days). Mice were also treated with B. longum subsp. infantis 35624 for 2 weeks before and during a 6-day S. Typhimurium challenge (10(6) CFU), or before injection of LPS. The small intestine was assessed for morphological changes, mRNA expression of cytokines, and activity of the brush border enzymes sucrase-isomaltase, maltase, and alkaline phosphatase.

Results: S. Typhimurium infection significantly reduced the activity of all brush border enzymes in a dose- and time-dependent manner (P<0.05). This also occurred following injection of LPS. Pre-treatment with B. longum subsp. infantis 35624 prevented weight loss, protected brush border enzyme activity, reduced the small intestinal damage, and inhibited the increase in interleukin (IL)-10 and IL-8 expression due to Salmonella challenge.

Conclusions: Salmonella infection reduces the small intestinal brush border enzyme activity in mice, with the level of reduction and associated weight loss increasing with dose and duration of infection. B. longum subsp. infantis 35624 treatment attenuated the effect of Salmonella infection on brush border enzyme activity and weight loss, which may be due to modulation of the host immune response.

No MeSH data available.


Related in: MedlinePlus